LOCUS       sequence-298318-        8101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral CaTCH barcoding plasmid harboring a single barcode 
            cassette (BC1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, 
            Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber 
            J, Obenauf AC
  TITLE     Isolating live cell clones from barcoded populations using 
            CRISPRa-inducible reporters.
  JOURNAL   Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0.
  PUBMED    32719478
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0."
COMMENT     Sequence Label: CaTCH BC1
FEATURES             Location/Qualifiers
     source          1..8101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             105..285
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    332..457
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    950..1183
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     1793..1817
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1896..1905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1902..1904,1905..1907,1908..2621)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1902..1904,1905..1907,1908..2618)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2663..2780
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2849..3060
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             join(3097..3099,3100..3102,3103..3816)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     misc_feature    3831..4419
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3884..3904)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4302..4313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4491..4724
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4802..4923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4839..4858)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4893..4912
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4963..5098
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5118..5137)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5119..5137)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5147..5164)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5147..5163)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5156..5178)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5305..5760
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5392..5411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5602..5623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5786..5890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5891..5959,5960..6751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6109..6128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6922..7510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7411..7430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7664..7681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7798..7819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7834..7839,7840..7857,7858..7864)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7872..7888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7877..7899
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7896..7912
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7896..7912
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7931..7951
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7933..7951
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(7979..8101,1..104)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
       61 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
      121 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      181 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      241 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      301 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      361 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      421 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      481 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      541 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      601 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      661 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
      721 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
      781 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
      841 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
      901 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
      961 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1021 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1081 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1141 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1201 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1261 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1321 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1381 aattcaagca gaagacggca tacgagatgt gactggagtt cagacgtgtg ctcttccgat
     1441 ctattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1501 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1561 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1621 cacctcccaa ccccgagggg accctatcat atgctgggta ccctgtctag agcgaacggg
     1681 cgagcgcgcg caaatggcgg cgactagtga tatccgtctc tcggcgctgt gctcgttcca
     1741 cacaccggca ggagaagctt taggcgtgta cggtgggcgc ctataaaagc agagctcgtt
     1801 tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat accaactttc
     1861 cgtaccactt cctaccctcg taaactggag gatccgccac catggtgagc aagggcgagg
     1921 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1981 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2041 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2101 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2161 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2221 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2281 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2341 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2401 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2461 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2521 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2581 ccgccgggat cactctcggc atggacgagc tgtacaagta agaattctag atcttgagac
     2641 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2701 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2761 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     2821 ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2881 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     2941 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     3001 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     3061 gtgtcgtgac gcggatcgta ccccgaattt atgatcatgg tgagcaaggg cgaggagctg
     3121 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3181 agcgtgaggg gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
     3241 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagccacggc
     3301 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3361 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg cacctacaag
     3421 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3481 gtcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc
     3541 cacaacatct atatcatggc cgtcaagcag aagaacggca tcaaggtgaa cttcaagatc
     3601 cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3661 atcggcgacg gccccgtgct gctgcccgac agccactacc tgagcaccca gtccgtgctg
     3721 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttccg caccgccgcc
     3781 gggatcactc tcggcatgga cgagctgtac aagtaacgcg ttaagtcgac aatcaacctc
     3841 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3901 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3961 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4021 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4081 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4141 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4201 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4261 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4321 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4381 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     4441 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4501 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     4561 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4621 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4681 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     4741 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     4801 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4921 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     4981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5041 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5101 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     5161 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5221 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5281 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5341 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5401 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5461 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5521 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5581 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5641 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5701 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5761 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5821 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5881 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5941 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6001 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6061 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6121 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6181 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6241 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6301 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6361 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6421 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6481 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6541 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6601 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6661 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6721 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6781 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6841 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6901 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6961 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7021 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7081 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7141 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7201 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7261 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7321 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7381 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7441 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7501 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7561 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7621 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7681 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7741 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     7801 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     7861 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     7921 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     7981 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     8041 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     8101 g
//
