LOCUS       sequence-298320-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CaTCH barcoding plasmid harboring a single barcode 
            cassette (BC1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, 
            Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber 
            J, Obenauf AC
  TITLE     Isolating live cell clones from barcoded populations using 
            CRISPRa-inducible reporters.
  JOURNAL   Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0.
  PUBMED    32719478
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0."
COMMENT     Sequence Label: CaTCH BC1
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(7..9,10..12,13..726)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(52..73)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atgatcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
       61 ctggacggcg acgtaaacgg ccacaagttc agcgtgaggg gcgagggcga gggcgatgcc
      121 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      181 cccaccctcg tgaccaccct gagccacggc gtgcagtgct tcgcccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      301 atcttcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc gtcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtacaa cttcaacagc cacaacatct atatcatggc cgtcaagcag
      481 aagaacggca tcaaggtgaa cttcaagatc cgccacaacg tggaggacgg cagcgtgcag
      541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      601 agccactacc tgagcaccca gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac
      661 atggtcctgc tggagttccg caccgccgcc gggatcactc tcggcatgga cgagctgtac
      721 aagtaa
//
