LOCUS       sequence-298326-        4047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of N gene's CDS from 
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 N_nostop
FEATURES             Location/Qualifiers
     source          1..4047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(46..73)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(165..251)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     300..322
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     314..331
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     315..331
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    347..446
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(635..646)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(1704..1803)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1820..1839)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1821..1839)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1844..1860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1844..1860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2291..3082
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3175..3763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3664..3683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3917..3934
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
       61 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
      121 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
      181 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
      241 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
      301 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt
      361 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
      421 gccaactttg tacaaaaaag ttggcatgtc cgacaacggt ccccagaacc agcgtaacgc
      481 tccaaggatc accttcggtg gacctagcga ctccaccggc agcaaccaga acggagaacg
      541 ctccggagcc agaagcaagc agcgtaggcc acagggcctg cctaacaaca ccgccagctg
      601 gttcaccgct ctgacccagc acggaaagga agacctgaag ttcccaaggg gacagggcgt
      661 gccaatcaac accaacagct ctccagacga ccagatcgga tactacaggc gtgccaccag
      721 gcgcatcaga ggaggcgacg gaaagatgaa ggacctgtct cctaggtggt acttctacta
      781 cctgggcacc ggaccagagg ctggtctgcc atacggcgcc aacaaggacg gcatcatctg
      841 ggtggctacc gagggagctc tgaacacccc aaaggaccac atcggaaccc gtaaccctgc
      901 caacaacgct gccatcgtgc tgcagctgcc acagggaacc accctgccca agggattcta
      961 cgctgagggc tccaggggtg gaagccaggc tagctctcgt tcttccagcc gttctaggaa
     1021 cagctcccgc aactctaccc caggaagctc cagaggcacc agcccagctc gtatggccgg
     1081 caacggaggt gacgccgctc tggctctgct gctgctggac cgtctgaacc agctggaaag
     1141 caagatgtcc ggaaagggtc agcagcagca gggccagacc gtgaccaaga agtctgccgc
     1201 tgaggcctct aagaagccca ggcagaagag gaccgctacc aaggcctaca acgtgaccca
     1261 ggccttcgga agacgtggac ctgaacagac ccagggaaac ttcggcgacc aggagctgat
     1321 ccgccaggga accgactaca agcactggcc acagatcgct cagttcgccc cttctgctag
     1381 cgccttcttc ggaatgtcca ggatcggaat ggaggtgacc ccttccggca cctggctgac
     1441 ctacaccgga gctatcaagc tggacgacaa ggaccccaac ttcaaggacc aggtgatcct
     1501 gctgaacaag cacatcgacg cctacaagac cttcccacct accgagccaa agaaggacaa
     1561 gaagaagaag gccgacgaaa cccaggccct gccacagagg cagaagaagc agcagaccgt
     1621 gaccctgctg ccagctgctg acctggacga cttctccaag cagctgcagc agtctatgag
     1681 ctctgctgac tccacccagg cttacccaac tttcttgtac aaagttggca ttataagaaa
     1741 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     1801 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     1861 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     1921 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     1981 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     2041 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     2101 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     2161 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     2221 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     2281 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     2341 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     2401 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     2461 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     2521 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     2581 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     2641 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     2701 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     2761 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     2821 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     2881 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     2941 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     3001 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     3061 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     3121 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3301 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3841 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3901 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     3961 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     4021 ctggaaagcg ggcagtgagc gcaacgc
//
