LOCUS       sequence-298329-        6609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of S gene's CDS from 
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 S_nostop
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(158..185)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(277..363)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     412..434
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     426..443
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     427..443
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    459..558
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4378..4477)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4494..4513)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4495..4513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4518..4534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4518..4534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4965..5756
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5849..6437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6338..6357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6591..6608
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
       61 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
      121 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
      181 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
      241 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
      301 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
      361 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
      421 gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac
      481 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
      541 tgtacaaaaa agttggcatg ttcgtgttcc tggtgctgct gccactggtg agctcccagt
      601 gcgtgaacct gaccaccaga acccagctgc cacctgccta caccaactcc ttcaccaggg
      661 gcgtgtacta cccagacaag gtgttcagat ccagcgtgct gcactctacc caggacctgt
      721 tcctgccatt cttctctaac gtgacctggt tccacgccat ccacgtgtct ggaaccaacg
      781 gcaccaagag attcgacaac ccagtgctgc cattcaacga cggcgtgtac ttcgcttcta
      841 ccgagaagtc taacatcatc aggggttgga tcttcggaac caccctggac tctaagaccc
      901 agtctctgct gatcgtgaac aacgctacca acgtggtgat caaggtgtgc gagttccagt
      961 tctgcaacga cccattcctg ggagtgtact accacaagaa caacaagtct tggatggaaa
     1021 gcgagttcag ggtgtacagc tctgctaaca actgcacctt cgagtacgtg tctcagcctt
     1081 tcctgatgga cctggaagga aagcagggaa acttcaagaa cctgagagag ttcgtgttca
     1141 agaacatcga cggttacttc aagatataca gcaagcacac cccaatcaac ctggtgaggg
     1201 acctgccaca gggtttctct gctctggagc ccctggtgga cctgccaatc ggcatcaaca
     1261 tcaccaggtt ccagaccctg ctggctctgc acaggtctta cctgacccca ggcgacagca
     1321 gctctggatg gaccgccggc gccgctgctt actacgtggg atacctgcag ccaaggacct
     1381 tcctgctgaa gtacaacgaa aacggcacca tcaccgacgc cgtggactgc gctctggacc
     1441 cactgtctga gaccaagtgc accctgaagt ctttcaccgt ggaaaaggga atctaccaga
     1501 ccagcaactt ccgcgtgcag cccaccgagt ctatcgtgag attcccaaac atcaccaacc
     1561 tgtgcccatt cggagaagtg ttcaacgcta ccaggttcgc cagcgtgtac gcttggaaca
     1621 ggaagagaat cagcaactgc gtggccgact actctgtgct gtacaactct gcctccttct
     1681 ccaccttcaa gtgctacggc gtgtccccaa ccaagctgaa cgacctgtgc ttcaccaacg
     1741 tgtacgctga ctccttcgtg atcagaggag acgaagtgag acagatcgct cccggtcaga
     1801 ccggaaagat cgccgactac aactacaagc tgccagacga cttcaccggt tgcgtgatcg
     1861 cttggaactc taacaacctg gactctaagg tgggaggcaa ctacaactac ctgtacaggc
     1921 tgttccgcaa gagcaacctg aagcctttcg aaagggacat cagcaccgaa atctaccagg
     1981 ctggatctac cccatgcaac ggagtggaag gattcaactg ctacttccct ctgcagtctt
     2041 acggattcca gccaaccaac ggagtgggat accagccata cagagtggtg gtgctgtctt
     2101 tcgagctgct gcacgctcct gctaccgtgt gcggaccaaa gaagtctacc aacctggtga
     2161 agaacaagtg cgtgaacttc aacttcaacg gactgaccgg aaccggagtg ctgaccgaat
     2221 ccaacaagaa gttcctgcct ttccagcagt tcggtagaga catcgccgac accaccgacg
     2281 ctgtgagaga cccacagacc ctggaaatcc tggacatcac cccctgctct ttcggaggag
     2341 tgagcgtgat caccccagga accaacacct ccaaccaggt ggccgtgctg taccaggacg
     2401 tgaactgcac cgaagtgcca gtggccatcc acgctgacca gctgacccca acctggagag
     2461 tgtacagcac cggatctaac gtgttccaga cccgtgccgg ctgcctgatc ggagccgagc
     2521 acgtgaacaa ctcctacgaa tgcgacatcc caatcggagc tggcatctgc gcttcttacc
     2581 agacccagac caacagccca agaagggcca gatccgtggc ctctcagagc atcatcgcct
     2641 acaccatgag cctgggcgct gaaaacagcg tggcttacag caacaactct atcgccatcc
     2701 caaccaactt caccatctct gtgaccaccg aaatcctgcc agtgagcatg accaagacca
     2761 gcgtggactg caccatgtac atctgcggag actctaccga atgctctaac ctgctgctgc
     2821 agtacggcag cttctgcacc cagctgaacc gcgctctgac cggaatcgct gtggagcagg
     2881 acaagaacac ccaggaagtg ttcgctcagg tgaagcagat atacaagacc ccacctatca
     2941 aggacttcgg aggattcaac ttctctcaga tcctgcccga cccatctaag ccctctaaga
     3001 ggtctttcat cgaagacctg ctgttcaaca aggtgaccct ggccgacgcc ggtttcatca
     3061 agcagtacgg tgactgcctg ggagacatcg ccgctagaga cctgatctgc gcccagaagt
     3121 tcaacggtct gaccgtgctg ccaccactgc tgaccgacga gatgatcgcc cagtacacct
     3181 ctgccctgct ggctggaacc atcacctctg gttggacctt cggagctggc gccgccctgc
     3241 agatcccatt cgctatgcag atggcctacc gtttcaacgg tatcggagtg acccagaacg
     3301 tgctgtacga aaaccagaag ctgatcgcta accagttcaa ctctgctatc ggaaagatcc
     3361 aggactccct gtcctctacc gcttctgccc tgggaaagct gcaggacgtg gtgaaccaga
     3421 acgcccaggc cctgaacacc ctggtgaagc agctgtcttc taacttcgga gccatctcct
     3481 ctgtgctgaa cgacatcctg tctaggctgg acaaggtgga agccgaggtg cagatcgaca
     3541 gactgatcac cggaagactg cagtctctgc agacctacgt gacccagcag ctgatcaggg
     3601 ccgctgaaat cagagcttct gccaacctgg ctgccaccaa gatgtccgaa tgcgtgctgg
     3661 gccagtctaa gagggtggac ttctgcggaa agggatacca cctgatgtcc ttcccacagt
     3721 ctgctccaca cggagtggtg ttcctgcacg tgacctacgt gcccgcccag gaaaagaact
     3781 tcaccaccgc cccagctatc tgccacgacg gaaaggctca cttccccagg gagggagtgt
     3841 tcgtgtctaa cggaacccac tggttcgtga cccagaggaa cttctacgag ccacagatca
     3901 tcaccaccga caacaccttc gtgtctggaa actgcgacgt ggtgatcgga atcgtgaaca
     3961 acaccgtgta cgacccactg cagccagaac tggactcttt caaggaggaa ctggacaagt
     4021 acttcaagaa ccacacctct ccagacgtgg acctgggaga catctccgga atcaacgcct
     4081 ctgtggtgaa catccagaag gaaatcgaca gactgaacga ggtggccaag aacctgaacg
     4141 aatctctgat cgacctgcag gaactgggaa agtacgagca gtacatcaag tggccctggt
     4201 acatctggct gggtttcatc gctggtctga tcgctatcgt gatggtgacc atcatgctgt
     4261 gctgcatgac ctcttgctgc tcttgcctga agggatgctg ctcttgcgga tcttgctgca
     4321 agttcgacga ggacgactcc gagcctgtgc tgaagggtgt gaagctgcac tacacctacc
     4381 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     4441 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     4501 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     4561 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     4621 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     4681 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     4741 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     4801 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     4861 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     4921 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     4981 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     5041 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     5101 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     5161 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     5221 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     5281 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     5341 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     5401 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     5461 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     5521 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     5581 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     5641 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     5701 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     5761 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     5821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6421 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6481 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6541 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6601 tgagcgagg
//
