LOCUS       sequence-298360-        1400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 nsp8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1400)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046711
FEATURES             Location/Qualifiers
     source          1..1400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(834..852)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 cgtttctttt tcttgaaaat tttttttttt gatttttttc tctttcgatg acctcccatt
       61 gatatttaag ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc
      121 tattacaact ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagt
      181 tttaattaca aaatggctat cgccagtgaa ttttcctcat tacctagcta tgcagctttc
      241 gctacggctc aggaggccta cgaacaagct gtagccaacg gcgactctga agttgttctt
      301 aagaaattga agaagtcttt gaatgtcgcg aaatctgaat ttgatagaga tgctgctatg
      361 caacgtaagc tagaaaagat ggctgaccaa gcaatgaccc aaatgtacaa acaagccaga
      421 agtgaggaca agcgagctaa ggtgacttcc gctatgcaaa caatgctgtt cactatgttg
      481 agaaagttgg ataacgatgc tctgaacaat attataaaca atgccagaga tggttgtgtc
      541 cccctcaaca ttatcccatt aaccactgcc gctaaattga tggttgttat tccggattat
      601 aatacataca aaaacacttg cgacggtacc accttcacat acgcctctgc tctttgggaa
      661 atccaacagg tagtcgacgc tgactccaag atagttcaat tgagcgaaat ttctatggat
      721 aactcgccaa acctagcttg gccattaatc gtcactgcgt tgagagctaa tagtgcggtt
      781 aagttgcaat aaacaggccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc
      841 ttacattcac gccctcctcc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
      901 gaagtctagg tccctattta ttttttttaa tagttatgtt agtattaaga acgttattta
      961 tatttcaaat ttttcttttt tttctgtaca aagcggccgc cgaaccacta gtgcactgca
     1021 gtacacggtt tccttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa
     1081 ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa
     1141 attgcccagt attcttaacc caactgcaca gaacaaaaac gtgcaggaaa cgaagataaa
     1201 tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca
     1261 agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta
     1321 ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa
     1381 cacatgtgga tatcttgact
//
