LOCUS       sequence-298381-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 E (envelope).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046610
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..72
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(359..377)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gtattacttc ttattcaaat gtcataaaag tatcaacaaa aaattgttaa tatacctcta
       61 tactttaacg tcaaggagaa aaaactataa tgtattcttt tgttagcgag gaaacgggaa
      121 ctcttatagt aaactcggtg ctattattcc tggctttcgt cgtttttctc ttggttacct
      181 tagcaatttt gactgccttg aggttgtgtg cgtactgctg taatatcgtc aacgtttcat
      241 tggtcaagcc ttctttctac gtttactcca gagtcaaaaa tttgaactcc tctcgcgtcc
      301 cagatttatt ggtttaaaca ggcccctttt cctttgtcga tatcatgtaa ttagttatgt
      361 cacgcttaca ttcacgccct cctcccacat ccgctctaac cgaaaaggaa ggagttagac
      421 aacctgaagt ctaggtccct atttattttt tttaatagtt atgttagtat taagaacgtt
      481 atttatattt caaatttttc ttttttttct gtacaaagcg gccgccgaac cactagtgca
      541 ctgcagtaca cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga
      601 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
      661 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacgtgca ggaaacgaag
      721 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc
      781 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt
      841 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact
      901 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa
      961 ggcattatcc gccaagtaca attttt
//
