LOCUS       sequence-29841-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     Michael Rout Plasmids
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHO4NLS-GFP-PRA (109)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(123..836)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVHGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ccctaaacgt tagcactttg gcttggatca tctttaaggc tttggataaa accattgcgt
       61 tgatcagcat ttaagttagg catatttaag acttgataaa aagcattttg ttgagcaagc
      121 tttttgtaca attcatccat accatgggta ataccagcag cagtaacaaa ttctaacaag
      181 accatgtggt ctctcttttc gtttggatct ttggataagg cagattgagt ggataagtaa
      241 tggttgtctg gtaacaagac tggaccatca ccaattggag tattttgttg ataatggtca
      301 gctaattgaa cagaaccatc ttcaatgttg tgtctaattt tgaagttaac tttgatacca
      361 ttcttttgtt tgtcagccat gatgtaaaca ttgtgagagt tatagttgta ttccaatttg
      421 tgacctaaaa tgttaccatc ttctttaaaa tcaatacctt ttaattcgat tctattaact
      481 aaggtatcac cttcaaactt gacttcagct ctggtcttgt agttaccgtc atctttgaaa
      541 aaaatagttc tttcttgaac ataaccttct ggcatggcag acttgaaaaa gtcatgttgt
      601 ttcatatgat ctgggtatct cgcaaaacat tgaacaccat aaccgaaagt agtgactaag
      661 gttggccatg gaactggcaa tttaccagta gtacaaataa attttaaggt caatttaccg
      721 taagtagcat caccttcacc ttcaccggag acagaaaatt tgtgaccatg aacatcacca
      781 tccaattcaa ccaaaattgg gacaacacca gtgaataatt cttcaccttt agacatggct
      841 cccggcagaa taactttcgg atattggctt tggtttcggg ttccggtttc ggaagttggg
      901 ataa
//
