LOCUS       sequence-298429-        8712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, 
            Saarma M, Varjosalo M
  TITLE     PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and 
            Inhibits the RET Mutant MEN2A Oncogenic Potential.
  JOURNAL   iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871.
            Epub 2020 Jan 31.
  PUBMED    32062451
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-02-21"; volume: "23(2):100871. doi"; pages: " 
            10.1016/j.isci.2020.100871. Epub 2020 Jan 31"
COMMENT     Sequence Label: PTPRA-MAC
FEATURES             Location/Qualifiers
     source          1..8712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(88..107)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        279..658
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        659..862
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     813..833
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    864..882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    885..903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     913..937
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1080..1104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3493..3517)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3564..3587
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3624..3647
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3678..3704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3678..3704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3705..4052,4053..4055,4056..4664)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4708..4725)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4714..4938
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5071..5090)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5222..5269
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5277..6299
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5277..6299
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6429..6550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6466..6485)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6520..6539
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6599..6615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6599..6615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6612..6634)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6623..6639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6647..6653,6654..6671,6672..6677))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6692..6713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6830..6847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7001..7589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7081..7100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7760..8551,8552..8620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8383..8402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8621..8712,1..13))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
       61 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      121 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
      181 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
      241 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      301 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      361 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      421 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      481 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      541 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      601 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      661 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      721 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      781 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      841 ggaggtctat ataagcagag ctctccctat cagtgataga gatctcccta tcagtgatag
      901 agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
      961 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag cgtttaaact
     1021 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcaa
     1081 caagtttgta caaaaaagca ggctccacca tggattcctg gttcattctt gttctgctcg
     1141 gcagtggtct gatatgtgtc agtgccaaca atgctaccac agttgcacct tctgtaggaa
     1201 ttacaagatt aattaactca tcaacggcag aaccagttaa agaagaggcc aaaacttcaa
     1261 atccaacttc ttcactaact tctctttctg tggcaccaac attcagccca aatataactc
     1321 tgggacccac ctatttaacc actgtcaatt cttcagactc tgacaatggg accacaagaa
     1381 cagcaagcac caattctata ggcattacaa tttcaccaaa tggaacgtgg cttccagata
     1441 accagttcac ggatgccaga acagaaccct gggaggggaa ttccagcacc gcagcaacca
     1501 ctccagaaac tttccctcct tcagatgaga caccaattat tgcggtgatg gtggccctgt
     1561 cctctctgct agtgatcgtg tttattatca tagttttgta catgttaagg tttaagaaat
     1621 acaagcaagc tgggagccat tccaattctt tccgcttatc caacggccgc actgaggatg
     1681 tggagcccca gagtgtgcca cttctggcca gatccccaag caccaacagg aaatacccac
     1741 ccctgcccgt ggacaagctg gaagaggaaa ttaaccggag aatggcagac gacaataagc
     1801 tcttcaggga ggaattcaac gctctccctg catgtcctat ccaggccacc tgtgaggctg
     1861 cttccaagga ggaaaacaag gaaaaaaatc gatatgtaaa catcttgcct tatgaccact
     1921 ctagagtcca cctgacaccg gttgaagggg ttccagattc tgattacatc aatgcttcat
     1981 tcatcaacgg ttaccaagaa aagaacaaat tcattgctgc acaaggacca aaagaagaaa
     2041 cggtgaatga tttctggcgg atgatctggg aacaaaacac agccaccatc gtcatggtta
     2101 ccaacctgaa ggagagaaag gagtgcaagt gcgcccagta ctggccagac caaggctgct
     2161 ggacctatgg gaatattcgg gtgtctgtag aggatgtgac tgtcctggtg gactacacag
     2221 tacggaagtt ctgcatccag caggtgggcg acatgaccaa cagaaagcca cagcgcctca
     2281 tcactcagtt ccactttacc agctggccag actttggggt gccttttacc ccgatcggca
     2341 tgctcaagtt cctcaagaag gtgaaggcct gtaaccctca gtatgcaggg gccatcgtgg
     2401 tccactgcag tgcaggtgta gggcgtacag gtacctttgt cgtcattgat gccatgctgg
     2461 acatgatgca tacagaacgg aaggtggacg tgtatggctt tgtgagccgg atccgggcac
     2521 agcgctgcca gatggtgcaa accgatatgc agtatgtctt catataccaa gcccttctgg
     2581 agcattatct ctatggagat acagaactgg aagtgacctc tctagaaacc cacctgcaga
     2641 aaatttacaa caaaatccca gggaccagca acaatggatt agaggaggag tttaagaagt
     2701 taacatcaat caaaatccag aatgacaaga tgcggactgg aaaccttcca gccaacatga
     2761 agaagaaccg tgttttacag atcattccat atgaattcaa cagagtgatc attccagtta
     2821 agcggggcga agagaataca gactatgtga acgcatcctt tattgatggc taccggcaga
     2881 aggactccta tatcgccagc cagggccctc ttctccacac aattgaggac ttctggcgaa
     2941 tgatctggga gtggaaatcc tgctctatcg tgatgctaac agaactggag gagagaggcc
     3001 aggagaagtg tgcccagtac tggccatctg atggactggt gtcctatgga gatattacag
     3061 tggaactgaa gaaggaggag gaatgtgaga gctacaccgt ccgagacctc ctggtcacca
     3121 acaccaggga gaataagagc cggcagatcc ggcagttcca cttccatggc tggcctgaag
     3181 tgggcatccc cagtgacgga aagggcatga tcagcatcat cgccgccgtg cagaagcagc
     3241 agcagcagtc agggaaccac cccatcaccg tgcactgcag cgccggggca ggaaggacgg
     3301 ggaccttctg tgccctgagc accgtcctgg agcgtgtgaa agcagagggg attttggatg
     3361 tcttccagac tgtcaagagc ctgcggctac agaggccaca catggtccag acactggaac
     3421 agtatgagtt ctgctacaag gtggtgcagg agtatattga tgcattctca gattatgcca
     3481 acttcaagtt ggacccagct ttcttgtaca aagtggttga tatcaaggcc gccgacatca
     3541 ccagcctgta caagaaagtg gggtggagcc atccccagtt cgagaagggc ggaggatctg
     3601 gcggcggaag cggcggagga agctggtccc accctcagtt tgagaaaggc accgagctgg
     3661 gcagcaccat ggccagctac ccctacgacg tgcccgacta cgccaaggac aacaccgtgc
     3721 ccctgaagct gatcgccctg ctggccaacg gcgagttcca ctctggcgag cagctgggcg
     3781 agacactggg catgagcaga gccgccatca acaagcacat ccagaccctg cgggactggg
     3841 gcgtggacgt gttcacagtg cccggcaagg gctacagcct gcccgagccc atccagctgc
     3901 tgaacgccaa gcagatcctg ggccagctgg atggcggatc tgtggccgtg ctgcccgtga
     3961 tcgacagcac caaccagtat ctgctcgacc ggatcggcga gctgaagtcc ggcgacgcct
     4021 gtatcgccga gtaccagcag gccggcagag gcggcagggg cagaaagtgg ttcagcccct
     4081 tcggcgccaa cctgtacctg agcatgttct ggagactgga acagggccca gccgccgcta
     4141 ttggcctgag cctggtcatc ggcatcgtga tggccgaggt gctgcggaag ctgggcgccg
     4201 acaaagtgcg cgtgaagtgg cccaacgacc tgtatctgca ggaccggaag ctggccggca
     4261 tcctggtgga actgaccggc aaaacaggcg acgccgccca gatcgtgatc ggcgctggca
     4321 tcaacatggc catgcggcgg gtggaagaga gcgtggtcaa ccagggctgg atcaccctgc
     4381 aggaagccgg catcaacctg gaccggaaca ccctggccgc catgctgatc agagagctga
     4441 gagccgccct ggaactgttc gagcaggaag gcctggcccc ctacctgagc agatgggaga
     4501 agctggacaa cttcatcaac cggcccgtga aactgatcat cggcgacaaa gagatcttcg
     4561 gcatcagccg gggcatcgac aagcaggggg ccctgctcct ggaacaggac ggcatcatca
     4621 agccctggat gggcggcgag atcagcctgc ggagcgccga gaagtgatga ctcgagtcta
     4681 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4741 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4801 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4861 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4921 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     4981 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5041 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5101 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5161 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct
     5221 agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa
     5281 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     5341 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     5401 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     5461 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     5521 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     5581 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     5641 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     5701 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     5761 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     5821 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     5881 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     5941 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     6001 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     6061 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     6121 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     6181 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     6241 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc
     6301 acgtactacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6361 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6421 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6481 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6541 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6601 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6661 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6721 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6781 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6841 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6901 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6961 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7021 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7081 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7141 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7201 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7261 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7321 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7381 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7441 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7501 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7561 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7621 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7681 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7741 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7801 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7861 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7921 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7981 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8041 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8101 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8161 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8221 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8281 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8341 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8401 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8461 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8521 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8581 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8641 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8701 aaaataaaca aa
//
