LOCUS       sequence-298508-        8580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  StrepIII-HA-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, 
            Saarma M, Varjosalo M
  TITLE     PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and 
            Inhibits the RET Mutant MEN2A Oncogenic Potential.
  JOURNAL   iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871.
            Epub 2020 Jan 31.
  PUBMED    32062451
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-02-21"; volume: "23(2):100871. doi"; pages: " 
            10.1016/j.isci.2020.100871. Epub 2020 Jan 31"
COMMENT     Sequence Label: MEN2B-SHC
FEATURES             Location/Qualifiers
     source          1..8580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     255..279
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    422..446
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3663..3687)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3734..3757
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3794..3817
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3848..3874
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3848..3874
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3918..3935)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3924..4148
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4281..4300)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4432..4479
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4487..5509
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4487..5509
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5639..5760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5676..5695)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5730..5749
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5809..5825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5809..5825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5822..5844)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5833..5849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5857..5863,5864..5881,5882..5887))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5902..5923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6040..6057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6211..6799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6291..6310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6970..7761,7762..7830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7593..7612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7831..7935)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8010..8029)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8201..8580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
      241 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      301 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa
      361 cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc
      421 aacaagtttg tacaaaaaag ttggcaccat ggcgaaggcg acgtccggtg ccgcggggct
      481 gcgtctgctg ttgctgctgc tgctgccgct gctaggcaaa gtggcattgg gcctctactt
      541 ctcgagggat gcttactggg agaagctgta tgtggaccag gcggccggca cgcccttgct
      601 gtacgtccat gccctgcggg acgcccctga ggaggtgccc agcttccgcc tgggccagca
      661 tctctacggc acgtaccgca cacggctgca tgagaacaac tggatctgca tccaggagga
      721 caccggcctc ctctacctta accggagcct ggaccatagc tcctgggaga agctcagtgt
      781 ccgcaaccgc ggctttcccc tgctcaccgt ctacctcaag gtcttcctgt cacccacatc
      841 ccttcgtgag ggcgagtgcc agtggccagg ctgtgcccgc gtatacttct ccttcttcaa
      901 cacctccttt ccagcctgca gctccctcaa gccccgggag ctctgcttcc cagagacaag
      961 gccctccttc cgcattcggg agaaccgacc cccaggcacc ttccaccagt tccgcctgct
     1021 gcctgtgcag ttcttgtgcc ccaacatcag cgtggcctac aggctcctgg agggtgaggg
     1081 tctgcccttc cgctgcgccc cggacagcct ggaggtgagc acgcgctggg ccctggaccg
     1141 cgagcagcgg gagaagtacg agctggtggc cgtgtgcacc gtgcacgccg gcgcgcgcga
     1201 ggaggtggtg atggtgccct tcccggtgac cgtgtacgac gaggacgact cggcgcccac
     1261 cttccccgcg ggcgtcgaca ccgccagcgc cgtggtggag ttcaagcgga aggaggacac
     1321 cgtggtggcc acgctgcgtg tcttcgatgc agacgtggta cctgcatcag gggagctggt
     1381 gaggcggtac acaagcacgc tgctccccgg ggacacctgg gcccagcaga ccttccgggt
     1441 ggaacactgg cccaacgaga cctcggtcca ggccaacggc agcttcgtgc gggcgaccgt
     1501 acatgactat aggctggttc tcaaccggaa cctctccatc tcggagaacc gcaccatgca
     1561 gctggcggtg ctggtcaatg actcagactt ccagggccca ggagcgggcg tcctcttgct
     1621 ccacttcaac gtgtcggtgc tgccggtcag cctgcacctg cccagtacct actccctctc
     1681 cgtgagcagg agggctcgcc gatttgccca gatcgggaaa gtctgtgtgg aaaactgcca
     1741 ggcattcagt ggcatcaacg tccagtacaa gctgcattcc tctggtgcca actgcagcac
     1801 gctaggggtg gtcacctcag ccgaggacac ctcggggatc ctgtttgtga atgacaccaa
     1861 ggccctgcgg cggcccaagt gtgccgaact tcactacatg gtggtggcca ccgaccagca
     1921 gacctctagg caggcccagg cccagctgct tgtaacagtg gaggggtcat atgtggccga
     1981 ggaggcgggc tgccccctgt cctgtgcagt cagcaagaga cggctggagt gtgaggagtg
     2041 tggcggcctg ggctccccaa caggcaggtg tgagtggagg caaggagatg gcaaagggat
     2101 caccaggaac ttctccacct gctctcccag caccaagacc tgccccgacg gccactgcga
     2161 tgttgtggag acccaagaca tcaacatttg ccctcaggac tgcctccggg gcagcattgt
     2221 tgggggacac gagcctgggg agccccgggg gattaaagct ggctatggca cctgcaactg
     2281 cttccctgag gaggagaagt gcttctgcga gcccgaagac atccaggatc cactgtgcga
     2341 cgagctgtgc cgcacggtga tcgcagccgc tgtcctcttc tccttcatcg tctcggtgct
     2401 gctgtctgcc ttctgcatcc actgctacca caagtttgcc cacaagccac ccatctcctc
     2461 agctgagatg accttccgga ggcccgccca ggccttcccg gtcagctact cctcttccag
     2521 tgcccgccgg ccctcgctgg actccatgga gaaccaggtc tccgtggatg ccttcaagat
     2581 cctggaggat ccaaagtggg aattccctcg gaagaacttg gttcttggaa aaactctagg
     2641 agaaggcgaa tttggaaaag tggtcaaggc aacggccttc catctgaaag gcagagcagg
     2701 gtacaccacg gtggccgtga agatgctgaa agagaacgcc tccccgagtg agcttcgaga
     2761 cctgctgtca gagttcaacg tcctgaagca ggtcaaccac ccacatgtca tcaaattgta
     2821 tggggcctgc agccaggatg gcccgctcct cctcatcgtg gagtacgcca aatacggctc
     2881 cctgcggggc ttcctccgcg agagccgcaa agtggggcct ggctacctgg gcagtggagg
     2941 cagccgcaac tccagctccc tggaccaccc ggatgagcgg gccctcacca tgggcgacct
     3001 catctcattt gcctggcaga tctcacaggg gatgcagtat ctggccgaga tgaagctcgt
     3061 tcatcgggac ttggcagcca gaaacatcct ggtagctgag gggcggaaga tgaagatttc
     3121 ggatttcggc ttgtcccgag atgtttatga agaggattcg tacgtgaaga ggagccaggg
     3181 tcggattcca gttaaatgga cggcaattga atcccttttt gatcatatct acaccacgca
     3241 aagtgatgta tggtcttttg gtgtcctgct gtgggagatc gtgaccctag ggggaaaccc
     3301 ctatcctggg attcctcctg agcggctctt caaccttctg aagaccggcc accggatgga
     3361 gaggccagac aactgcagcg aggagatgta ctgcctgatg ctgcaatgct ggaagcagga
     3421 gccggacaaa aggccggtgt ttgcggacat cagcaaagac ctggagaaga tgatggttaa
     3481 gaggagagac tacttggacc ttgcggcgtc cactccatct gactccctga tttatgacga
     3541 cggcctctca gaggaggaga caccgctggt ggactgtaat aatgcccccc tccctcgagc
     3601 cctcccttcc acatggattg aaaacaaact ctatggtaga atttcccatg catttactag
     3661 attcccaact ttcttgtaca aagtggttga tatcaaggcc gccgacatca ccagcctgta
     3721 caagaaagtg gggtggagcc atccccagtt cgagaagggc ggaggatctg gcggcggaag
     3781 cggcggagga agctggtccc accctcagtt tgagaaaggc accgagctgg gcagcaccat
     3841 ggccagctac ccctacgacg tgcccgacta cgcctgatga ctcgagtcta gagggcccgt
     3901 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3961 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4021 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4081 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4141 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4201 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4261 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4321 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4381 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct
     4441 attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact
     4501 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     4561 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     4621 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     4681 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     4741 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     4801 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     4861 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     4921 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     4981 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     5041 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     5101 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     5161 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     5221 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     5281 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     5341 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     5401 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     5461 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg
     5521 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5581 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5641 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5701 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5761 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     5821 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5881 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5941 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6001 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6061 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6901 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6961 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7021 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7081 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7141 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7201 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7261 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7321 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7381 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7441 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7501 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7561 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7621 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7681 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7741 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7801 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7861 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7921 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     7981 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8041 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     8101 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     8161 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     8221 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     8281 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     8341 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     8401 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     8461 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     8521 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
