LOCUS       sequence-298523-        2979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  direct fusion of biosynthetic enzymes 4CL and STS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala
            R, Gradisar H
  TITLE     Metabolic enzyme clustering by coiled coils improves the 
            biosynthesis of resveratrol and mevalonate.
  JOURNAL   AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5.
  PUBMED    32448937
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AMB 
            Express."; date: "2020-05-24"; volume: "10(1):97. doi"; pages: " 
            10.1186/s13568-020-01031-5"
COMMENT     Sequence Label: pET19b_4CL:STS (DF)
FEATURES             Location/Qualifiers
     source          1..2979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..36
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             52..66
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 atgggccatc atcatcatca tcatcatcat catcacagca gcggccatat cgacgacgac
       61 gacaagcata tgctcgaggc accgcaagaa caggcagtta gccaggttat ggaaaaacag
      121 agcaacaata acaacagcga cgtgattttt cgtagcaaac tgccggatat ctatattccg
      181 aatcatctga gcctgcacga ttatatcttt cagaacatta gcgagtttgc caccaaaccg
      241 tgtctgatta atggtccgac cggtcatgtt tatacctata gtgatgttca tgtgatcagc
      301 cgtcagattg cagccaattt tcataaactg ggtgtgaatc agaatgacgt tgttatgctg
      361 ctgctgccga attgtccgga atttgttctg agctttctgg cagccagctt tcgtggtgca
      421 accgcaaccg cagcaaatcc gttttttaca ccggcagaaa ttgcaaaaca ggcaaaagca
      481 agcaacacca aactgattat taccgaagca cgttacgtgg acaaaatcaa accgctgcaa
      541 aatgatgatg gtgttgtgat tgtttgcatc gatgataatg aaagcgttcc gattccggaa
      601 ggttgtctgc gttttaccga actgacccag agcaccaccg aagcaagcga agttattgat
      661 agcgttgaaa ttagtccgga tgatgttgtt gcactgccgt atagcagcgg caccaccggt
      721 ctgccgaaag gtgttatgct gacccataaa ggtctggtta ccagcgttgc acagcaggtt
      781 gatggtgaaa atccgaatct gtattttcat agcgacgatg ttattctgtg tgtgctgccg
      841 atgtttcata tttatgcact gaatagcatt atgctgtgtg gtctgcgtgt tggtgcagca
      901 attctgatta tgccgaaatt tgaaattaat ctgctgctgg aactgatcca gcgttgtaaa
      961 gttaccgttg caccgatggt tccgcctatt gttctggcaa ttgcaaaaag cagcgaaacc
     1021 gaaaaatatg atctgagcag cattcgtgtt gttaaaagcg gtgcagctcc gctgggtaaa
     1081 gaactggaag atgcagttaa tgccaaattt ccgaatgcaa aactgggtca gggttatggt
     1141 atgaccgaag caggtccggt tctggcaatg agcctgggtt ttgcaaaaga accgtttccg
     1201 gttaaaagtg gtgcatgtgg caccgttgtt cgtaatgcag aaatgaaaat tgttgatccg
     1261 gataccggtg atagcctgag ccgtaatcag cctggtgaaa tttgtattcg tggtcaccag
     1321 attatgaaag gctatctgaa taatccggca gcaaccgcag aaaccattga taaagatggt
     1381 tggctgcata caggtgatat tggtctgatt gatgatgatg acgaactgtt tattgtggat
     1441 cgtctgaaag agctgatcaa atacaaaggt tttcaggttg caccggcaga actggaagca
     1501 ctgctgattg gtcatccgga tattaccgat gttgcagttg ttgcaatgaa agaagaagca
     1561 gcaggcgaag ttccggttgc atttgttgtg aaaagcaaag atagcgaact gagcgaagat
     1621 gatgttaaac agtttgttag caaacaggtg gtgttctaca aacgcatcaa caaagtgttt
     1681 tttaccgaga gcattccgaa agcaccgagc ggtaaaattc tgcgtaaaga tctgcgtgca
     1741 aaactggcaa atggtctggg tgggggctcc ggctccggct ccggcggctc cgcaagcgtt
     1801 gaagaatttc gtaatgcaca gcgtgcaaaa ggtccggcaa ccattctggc aattggcacc
     1861 gcaacaccgg atcattgtgt ttatcagagc gattatgccg attattactt taaagtgacc
     1921 aaaagcgaac acatgaccgc actgaaaaag aaatttaacc gcatctgcga taaaagcatg
     1981 atcaaaaaac gctatatcca cctgaccgaa gaaatgctgg aagaacatcc gaatattggt
     2041 gcatatatgg caccgagcct gaatattcgt caagaaatta tcaccgcaga agttccgaaa
     2101 ctgggtaaag aagcagcact gaaagccctg aaagaatggg gtcagccgaa aagcaaaatt
     2161 acccatctgg ttttttgtac caccagtggt gttgaaatgc ctggtgcaga ttataaactg
     2221 gcaaatctgc tgggtctgga accgagcgtt cgtcgtgtta tgctgtatca tcagggttgt
     2281 tatgccggtg gcaccgttct gcgtaccgca aaagatctgg cagaaaataa tgccggtgca
     2341 cgtgttctgg ttgtttgtag cgaaattacc gttgttacct ttcgtggtcc gagcgaagat
     2401 gcactggata gcctggttgg tcaggcactg tttggtgatg gtagcgcagc agttattgtt
     2461 ggtagcgatc cggatattag cattgaacgt ccgctgtttc agctggttag cgcagcacag
     2521 acctttattc cgaatagtgc cggtgcaatt gcaggtaatc tgcgtgaagt tggtctgacc
     2581 tttcatctgt ggccgaatgt tccgaccctg attagcgaaa atgttgaaaa atgtctgacc
     2641 caggcatttg atccgctggg tattagcgat tggaatagcc tgttttggat tgcacatccg
     2701 ggtggtccgg caattctgga tgcagttgaa gcaaaactga acctggacaa aaagaaactg
     2761 gaagcaaccc gtcatgttct gagcgaatat ggtaatatga gcagcgcatg tgttctgttt
     2821 attctggatg aaatgcgcaa aaaaagcctg aaaggtgaac gtgcaaccac cggtgaaggt
     2881 ctggattggg gtgttctgtt tggttttggt ccgggtctga ccattgaaac cgttgttctg
     2941 catagcattc cgatggttac caatggatcc ggctgctaa
//
