LOCUS       sequence-298525-        1764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses biosynthetic enzyme 4CL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala
            R, Gradisar H
  TITLE     Metabolic enzyme clustering by coiled coils improves the 
            biosynthesis of resveratrol and mevalonate.
  JOURNAL   AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5.
  PUBMED    32448937
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AMB 
            Express."; date: "2020-05-24"; volume: "10(1):97. doi"; pages: " 
            10.1186/s13568-020-01031-5"
COMMENT     Sequence Label: pET19b_4CL
FEATURES             Location/Qualifiers
     source          1..1764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..36
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             52..66
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 atgggccatc atcatcatca tcatcatcat catcacagca gcggccatat cgacgacgac
       61 gacaagcata tgctcgaggc accgcaagaa caggcagtta gccaggttat ggaaaaacag
      121 agcaacaata acaacagcga cgtgattttt cgtagcaaac tgccggatat ctatattccg
      181 aatcatctga gcctgcacga ttatatcttt cagaacatta gcgagtttgc caccaaaccg
      241 tgtctgatta atggtccgac cggtcatgtt tatacctata gtgatgttca tgtgatcagc
      301 cgtcagattg cagccaattt tcataaactg ggtgtgaatc agaatgacgt tgttatgctg
      361 ctgctgccga attgtccgga atttgttctg agctttctgg cagccagctt tcgtggtgca
      421 accgcaaccg cagcaaatcc gttttttaca ccggcagaaa ttgcaaaaca ggcaaaagca
      481 agcaacacca aactgattat taccgaagca cgttacgtgg acaaaatcaa accgctgcaa
      541 aatgatgatg gtgttgtgat tgtttgcatc gatgataatg aaagcgttcc gattccggaa
      601 ggttgtctgc gttttaccga actgacccag agcaccaccg aagcaagcga agttattgat
      661 agcgttgaaa ttagtccgga tgatgttgtt gcactgccgt atagcagcgg caccaccggt
      721 ctgccgaaag gtgttatgct gacccataaa ggtctggtta ccagcgttgc acagcaggtt
      781 gatggtgaaa atccgaatct gtattttcat agcgacgatg ttattctgtg tgtgctgccg
      841 atgtttcata tttatgcact gaatagcatt atgctgtgtg gtctgcgtgt tggtgcagca
      901 attctgatta tgccgaaatt tgaaattaat ctgctgctgg aactgatcca gcgttgtaaa
      961 gttaccgttg caccgatggt tccgcctatt gttctggcaa ttgcaaaaag cagcgaaacc
     1021 gaaaaatatg atctgagcag cattcgtgtt gttaaaagcg gtgcagctcc gctgggtaaa
     1081 gaactggaag atgcagttaa tgccaaattt ccgaatgcaa aactgggtca gggttatggt
     1141 atgaccgaag caggtccggt tctggcaatg agcctgggtt ttgcaaaaga accgtttccg
     1201 gttaaaagtg gtgcatgtgg caccgttgtt cgtaatgcag aaatgaaaat tgttgatccg
     1261 gataccggtg atagcctgag ccgtaatcag cctggtgaaa tttgtattcg tggtcaccag
     1321 attatgaaag gctatctgaa taatccggca gcaaccgcag aaaccattga taaagatggt
     1381 tggctgcata caggtgatat tggtctgatt gatgatgatg acgaactgtt tattgtggat
     1441 cgtctgaaag agctgatcaa atacaaaggt tttcaggttg caccggcaga actggaagca
     1501 ctgctgattg gtcatccgga tattaccgat gttgcagttg ttgcaatgaa agaagaagca
     1561 gcaggcgaag ttccggttgc atttgttgtg aaaagcaaag atagcgaact gagcgaagat
     1621 gatgttaaac agtttgttag caaacaggtg gtgttctaca aacgcatcaa caaagtgttt
     1681 tttaccgaga gcattccgaa agcaccgagc ggtaaaattc tgcgtaaaga tctgcgtgca
     1741 aaactggcaa atggtctggg ttaa
//
