LOCUS       sequence-298528-        1872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of biosynthetic enzymes 4CL and STS in fusion with CC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala
            R, Gradisar H
  TITLE     Metabolic enzyme clustering by coiled coils improves the 
            biosynthesis of resveratrol and mevalonate.
  JOURNAL   AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5.
  PUBMED    32448937
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AMB 
            Express."; date: "2020-05-24"; volume: "10(1):97. doi"; pages: " 
            10.1186/s13568-020-01031-5"
COMMENT     Sequence Label: pET19b_4CL:P3S + STS:AP4S
FEATURES             Location/Qualifiers
     source          1..1872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1807..1821)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(1837..1866)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttaatagcca tatttcagtt cctggttttt ttctttcagt tcgctgtttt tctgttccag
       61 ctggctaatt tcttcttcca gctgctgaat ttcatcttcc gggctgctgc cgctgccacc
      121 cagaccattt gccagttttg cacgcagatc tttacgcaga attttaccgc tcggtgcttt
      181 cggaatgctc tcggtaaaaa acactttgtt gatgcgtttg tagaacacca cctgtttgct
      241 aacaaactgt ttaacatcat cttcgctcag ttcgctatct ttgcttttca caacaaatgc
      301 aaccggaact tcgcctgctg cttcttcttt cattgcaaca actgcaacat cggtaatatc
      361 cggatgacca atcagcagtg cttccagttc tgccggtgca acctgaaaac ctttgtattt
      421 gatcagctct ttcagacgat ccacaataaa cagttcgtca tcatcatcaa tcagaccaat
      481 atcacctgta tgcagccaac catctttatc aatggtttct gcggttgctg ccggattatt
      541 cagatagcct ttcataatct ggtgaccacg aatacaaatt tcaccaggct gattacggct
      601 caggctatca ccggtatccg gatcaacaat tttcatttct gcattacgaa caacggtgcc
      661 acatgcacca cttttaaccg gaaacggttc ttttgcaaaa cccaggctca ttgccagaac
      721 cggacctgct tcggtcatac cataaccctg acccagtttt gcattcggaa atttggcatt
      781 aactgcatct tccagttctt tacccagcgg agctgcaccg cttttaacaa cacgaatgct
      841 gctcagatca tatttttcgg tttcgctgct ttttgcaatt gccagaacaa taggcggaac
      901 catcggtgca acggtaactt tacaacgctg gatcagttcc agcagcagat taatttcaaa
      961 tttcggcata atcagaattg ctgcaccaac acgcagacca cacagcataa tgctattcag
     1021 tgcataaata tgaaacatcg gcagcacaca cagaataaca tcgtcgctat gaaaatacag
     1081 attcggattt tcaccatcaa cctgctgtgc aacgctggta accagacctt tatgggtcag
     1141 cataacacct ttcggcagac cggtggtgcc gctgctatac ggcagtgcaa caacatcatc
     1201 cggactaatt tcaacgctat caataacttc gcttgcttcg gtggtgctct gggtcagttc
     1261 ggtaaaacgc agacaacctt ccggaatcgg aacgctttca ttatcatcga tgcaaacaat
     1321 cacaacacca tcatcatttt gcagcggttt gattttgtcc acgtaacgtg cttcggtaat
     1381 aatcagtttg gtgttgcttg cttttgcctg ttttgcaatt tctgccggtg taaaaaacgg
     1441 atttgctgcg gttgcggttg caccacgaaa gctggctgcc agaaagctca gaacaaattc
     1501 cggacaattc ggcagcagca gcataacaac gtcattctga ttcacaccca gtttatgaaa
     1561 attggctgca atctgacggc tgatcacatg aacatcacta taggtataaa catgaccggt
     1621 cggaccatta atcagacacg gtttggtggc aaactcgcta atgttctgaa agatataatc
     1681 gtgcaggctc agatgattcg gaatatagat atccggcagt ttgctacgaa aaatcacgtc
     1741 gctgttgtta ttgttgctct gtttttccat aacctggcta actgcctgtt cttgcggtgc
     1801 catatgcttg tcgtcgtcgt cgatatggcc gctgctgtga tgatgatgat gatgatgatg
     1861 atgatggccc at
//
