LOCUS       sequence-298529-        1359 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of biosynthetic enzymes 4CL and STS in fusion with CC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala
            R, Gradisar H
  TITLE     Metabolic enzyme clustering by coiled coils improves the 
            biosynthesis of resveratrol and mevalonate.
  JOURNAL   AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5.
  PUBMED    32448937
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AMB 
            Express."; date: "2020-05-24"; volume: "10(1):97. doi"; pages: " 
            10.1186/s13568-020-01031-5"
COMMENT     Sequence Label: pET19b_4CL:P3S + STS:AP4S
FEATURES             Location/Qualifiers
     source          1..1359
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1294..1308)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(1324..1353)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttagccgtat ttaatactct gcaacttttg cttgatttgt tgtaatttct gttcattttg
       61 ttgcagttcc tcttcgttgg attgaagctc gtcctctggc gagctgccgc tgccattggt
      121 aaccatcgga atgctatgca gaacaacggt ttcaatggtc agacccggac caaaaccaaa
      181 cagaacaccc caatccagac cttcaccggt ggttgcacgt tcacctttca ggcttttttt
      241 gcgcatttca tccagaataa acagaacaca tgcgctgctc atattaccat attcgctcag
      301 aacatgacgg gttgcttcca gtttcttttt gtccaggttc agttttgctt caactgcatc
      361 cagaattgcc ggaccacccg gatgtgcaat ccaaaacagg ctattccaat cgctaatacc
      421 cagcggatca aatgcctggg tcagacattt ttcaacattt tcgctaatca gggtcggaac
      481 attcggccac agatgaaagg tcagaccaac ttcacgcaga ttacctgcaa ttgcaccggc
      541 actattcgga ataaaggtct gtgctgcgct aaccagctga aacagcggac gttcaatgct
      601 aatatccgga tcgctaccaa caataactgc tgcgctacca tcaccaaaca gtgcctgacc
      661 aaccaggcta tccagtgcat cttcgctcgg accacgaaag gtaacaacgg taatttcgct
      721 acaaacaacc agaacacgtg caccggcatt attttctgcc agatcttttg cggtacgcag
      781 aacggtgcca ccggcataac aaccctgatg atacagcata acacgacgaa cgctcggttc
      841 cagacccagc agatttgcca gtttataatc tgcaccaggc atttcaacac cactggtggt
      901 acaaaaaacc agatgggtaa ttttgctttt cggctgaccc cattctttca gggctttcag
      961 tgctgcttct ttacccagtt tcggaacttc tgcggtgata atttcttgac gaatattcag
     1021 gctcggtgcc atatatgcac caatattcgg atgttcttcc agcatttctt cggtcaggtg
     1081 gatatagcgt tttttgatca tgcttttatc gcagatgcgg ttaaatttct ttttcagtgc
     1141 ggtcatgtgt tcgcttttgg tcactttaaa gtaataatcg gcataatcgc tctgataaac
     1201 acaatgatcc ggtgttgcgg tgccaattgc cagaatggtt gccggacctt ttgcacgctg
     1261 tgcattacga aattcttcaa cgcttgccat atgcttgtcg tcgtcgtcga tatggccgct
     1321 gctgtgatga tgatgatgat gatgatgatg atggcccat
//
