LOCUS       sequence-298530-        1866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of biosynthetic enzymes 4CL and STS in fusion with CC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala
            R, Gradisar H
  TITLE     Metabolic enzyme clustering by coiled coils improves the 
            biosynthesis of resveratrol and mevalonate.
  JOURNAL   AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5.
  PUBMED    32448937
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AMB 
            Express."; date: "2020-05-24"; volume: "10(1):97. doi"; pages: " 
            10.1186/s13568-020-01031-5"
COMMENT     Sequence Label: pET19b_4CL:P7S + STS:P8S
FEATURES             Location/Qualifiers
     source          1..1866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1801..1815)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(1831..1860)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttaaccgtac tttaattgtt gattcttttc ctccaactga gagatttcct gtttaagctg
       61 actgttcttc tcttccaacg actgaatctc gtcctctggt gagccgctgc cacccagacc
      121 atttgccagt tttgcacgca gatctttacg cagaatttta ccgctcggtg ctttcggaat
      181 gctctcggta aaaaacactt tgttgatgcg tttgtagaac accacctgtt tgctaacaaa
      241 ctgtttaaca tcatcttcgc tcagttcgct atctttgctt ttcacaacaa atgcaaccgg
      301 aacttcgcct gctgcttctt ctttcattgc aacaactgca acatcggtaa tatccggatg
      361 accaatcagc agtgcttcca gttctgccgg tgcaacctga aaacctttgt atttgatcag
      421 ctctttcaga cgatccacaa taaacagttc gtcatcatca tcaatcagac caatatcacc
      481 tgtatgcagc caaccatctt tatcaatggt ttctgcggtt gctgccggat tattcagata
      541 gcctttcata atctggtgac cacgaataca aatttcacca ggctgattac ggctcaggct
      601 atcaccggta tccggatcaa caattttcat ttctgcatta cgaacaacgg tgccacatgc
      661 accactttta accggaaacg gttcttttgc aaaacccagg ctcattgcca gaaccggacc
      721 tgcttcggtc ataccataac cctgacccag ttttgcattc ggaaatttgg cattaactgc
      781 atcttccagt tctttaccca gcggagctgc accgctttta acaacacgaa tgctgctcag
      841 atcatatttt tcggtttcgc tgctttttgc aattgccaga acaataggcg gaaccatcgg
      901 tgcaacggta actttacaac gctggatcag ttccagcagc agattaattt caaatttcgg
      961 cataatcaga attgctgcac caacacgcag accacacagc ataatgctat tcagtgcata
     1021 aatatgaaac atcggcagca cacacagaat aacatcgtcg ctatgaaaat acagattcgg
     1081 attttcacca tcaacctgct gtgcaacgct ggtaaccaga cctttatggg tcagcataac
     1141 acctttcggc agaccggtgg tgccgctgct atacggcagt gcaacaacat catccggact
     1201 aatttcaacg ctatcaataa cttcgcttgc ttcggtggtg ctctgggtca gttcggtaaa
     1261 acgcagacaa ccttccggaa tcggaacgct ttcattatca tcgatgcaaa caatcacaac
     1321 accatcatca ttttgcagcg gtttgatttt gtccacgtaa cgtgcttcgg taataatcag
     1381 tttggtgttg cttgcttttg cctgttttgc aatttctgcc ggtgtaaaaa acggatttgc
     1441 tgcggttgcg gttgcaccac gaaagctggc tgccagaaag ctcagaacaa attccggaca
     1501 attcggcagc agcagcataa caacgtcatt ctgattcaca cccagtttat gaaaattggc
     1561 tgcaatctga cggctgatca catgaacatc actataggta taaacatgac cggtcggacc
     1621 attaatcaga cacggtttgg tggcaaactc gctaatgttc tgaaagatat aatcgtgcag
     1681 gctcagatga ttcggaatat agatatccgg cagtttgcta cgaaaaatca cgtcgctgtt
     1741 gttattgttg ctctgttttt ccataacctg gctaactgcc tgttcttgcg gtgccatatg
     1801 cttgtcgtcg tcgtcgatat ggccgctgct gtgatgatga tgatgatgat gatgatgatg
     1861 gcccat
//
