LOCUS       sequence-298531-        1356 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of biosynthetic enzymes 4CL and STS in fusion with CC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala
            R, Gradisar H
  TITLE     Metabolic enzyme clustering by coiled coils improves the 
            biosynthesis of resveratrol and mevalonate.
  JOURNAL   AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5.
  PUBMED    32448937
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AMB 
            Express."; date: "2020-05-24"; volume: "10(1):97. doi"; pages: " 
            10.1186/s13568-020-01031-5"
COMMENT     Sequence Label: pET19b_4CL:P7S + STS:P8S
FEATURES             Location/Qualifiers
     source          1..1356
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1291..1305)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(1321..1350)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttacccgtac tccagttgtg agttttcttc ctttaactgt tggattttct gctctaactg
       61 ttgattttcc tctttaagct gtgaaatctt gtcctctggt gagccgctgc cattggtaac
      121 catcggaatg ctatgcagaa caacggtttc aatggtcaga cccggaccaa aaccaaacag
      181 aacaccccaa tccagacctt caccggtggt tgcacgttca cctttcaggc tttttttgcg
      241 catttcatcc agaataaaca gaacacatgc gctgctcata ttaccatatt cgctcagaac
      301 atgacgggtt gcttccagtt tctttttgtc caggttcagt tttgcttcaa ctgcatccag
      361 aattgccgga ccacccggat gtgcaatcca aaacaggcta ttccaatcgc taatacccag
      421 cggatcaaat gcctgggtca gacatttttc aacattttcg ctaatcaggg tcggaacatt
      481 cggccacaga tgaaaggtca gaccaacttc acgcagatta cctgcaattg caccggcact
      541 attcggaata aaggtctgtg ctgcgctaac cagctgaaac agcggacgtt caatgctaat
      601 atccggatcg ctaccaacaa taactgctgc gctaccatca ccaaacagtg cctgaccaac
      661 caggctatcc agtgcatctt cgctcggacc acgaaaggta acaacggtaa tttcgctaca
      721 aacaaccaga acacgtgcac cggcattatt ttctgccaga tcttttgcgg tacgcagaac
      781 ggtgccaccg gcataacaac cctgatgata cagcataaca cgacgaacgc tcggttccag
      841 acccagcaga tttgccagtt tataatctgc accaggcatt tcaacaccac tggtggtaca
      901 aaaaaccaga tgggtaattt tgcttttcgg ctgaccccat tctttcaggg ctttcagtgc
      961 tgcttcttta cccagtttcg gaacttctgc ggtgataatt tcttgacgaa tattcaggct
     1021 cggtgccata tatgcaccaa tattcggatg ttcttccagc atttcttcgg tcaggtggat
     1081 atagcgtttt ttgatcatgc ttttatcgca gatgcggtta aatttctttt tcagtgcggt
     1141 catgtgttcg cttttggtca ctttaaagta ataatcggca taatcgctct gataaacaca
     1201 atgatccggt gttgcggtgc caattgccag aatggttgcc ggaccttttg cacgctgtgc
     1261 attacgaaat tcttcaacgc ttgccatatg cttgtcgtcg tcgtcgatat ggccgctgct
     1321 gtgatgatga tgatgatgat gatgatgatg gcccat
//
