LOCUS       sequence-298534-        1863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of biosynthetic enzymes 4CL and STS in fusion with CC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala
            R, Gradisar H
  TITLE     Metabolic enzyme clustering by coiled coils improves the 
            biosynthesis of resveratrol and mevalonate.
  JOURNAL   AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5.
  PUBMED    32448937
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AMB 
            Express."; date: "2020-05-24"; volume: "10(1):97. doi"; pages: " 
            10.1186/s13568-020-01031-5"
COMMENT     Sequence Label: pET19b_4CL:P5SHb + STS:P6SHb
FEATURES             Location/Qualifiers
     source          1..1863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1798..1812)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(1828..1857)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttattcttcc agatatttaa tctcacgttt caattcttca tttttttctt ttaactcgcg
       61 gattttctct tctaactcac gattttcgtc ttcaggactg ccgctgccac ccagaccatt
      121 tgccagtttt gcacgcagat ctttacgcag aattttaccg ctcggtgctt tcggaatgct
      181 ctcggtaaaa aacactttgt tgatgcgttt gtagaacacc acctgtttgc taacaaactg
      241 tttaacatca tcttcgctca gttcgctatc tttgcttttc acaacaaatg caaccggaac
      301 ttcgcctgct gcttcttctt tcattgcaac aactgcaaca tcggtaatat ccggatgacc
      361 aatcagcagt gcttccagtt ctgccggtgc aacctgaaaa cctttgtatt tgatcagctc
      421 tttcagacga tccacaataa acagttcgtc atcatcatca atcagaccaa tatcacctgt
      481 atgcagccaa ccatctttat caatggtttc tgcggttgct gccggattat tcagatagcc
      541 tttcataatc tggtgaccac gaatacaaat ttcaccaggc tgattacggc tcaggctatc
      601 accggtatcc ggatcaacaa ttttcatttc tgcattacga acaacggtgc cacatgcacc
      661 acttttaacc ggaaacggtt cttttgcaaa acccaggctc attgccagaa ccggacctgc
      721 ttcggtcata ccataaccct gacccagttt tgcattcgga aatttggcat taactgcatc
      781 ttccagttct ttacccagcg gagctgcacc gcttttaaca acacgaatgc tgctcagatc
      841 atatttttcg gtttcgctgc tttttgcaat tgccagaaca ataggcggaa ccatcggtgc
      901 aacggtaact ttacaacgct ggatcagttc cagcagcaga ttaatttcaa atttcggcat
      961 aatcagaatt gctgcaccaa cacgcagacc acacagcata atgctattca gtgcataaat
     1021 atgaaacatc ggcagcacac acagaataac atcgtcgcta tgaaaataca gattcggatt
     1081 ttcaccatca acctgctgtg caacgctggt aaccagacct ttatgggtca gcataacacc
     1141 tttcggcaga ccggtggtgc cgctgctata cggcagtgca acaacatcat ccggactaat
     1201 ttcaacgcta tcaataactt cgcttgcttc ggtggtgctc tgggtcagtt cggtaaaacg
     1261 cagacaacct tccggaatcg gaacgctttc attatcatcg atgcaaacaa tcacaacacc
     1321 atcatcattt tgcagcggtt tgattttgtc cacgtaacgt gcttcggtaa taatcagttt
     1381 ggtgttgctt gcttttgcct gttttgcaat ttctgccggt gtaaaaaacg gatttgctgc
     1441 ggttgcggtt gcaccacgaa agctggctgc cagaaagctc agaacaaatt ccggacaatt
     1501 cggcagcagc agcataacaa cgtcattctg attcacaccc agtttatgaa aattggctgc
     1561 aatctgacgg ctgatcacat gaacatcact ataggtataa acatgaccgg tcggaccatt
     1621 aatcagacac ggtttggtgg caaactcgct aatgttctga aagatataat cgtgcaggct
     1681 cagatgattc ggaatataga tatccggcag tttgctacga aaaatcacgt cgctgttgtt
     1741 attgttgctc tgtttttcca taacctggct aactgcctgt tcttgcggtg ccatatgctt
     1801 gtcgtcgtcg tcgatatggc cgctgctgtg atgatgatga tgatgatgat gatgatggcc
     1861 cat
//
