LOCUS       sequence-298553-        5580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses myc-tagged nucleocapsid (N) from SARS-CoV (1) in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  TITLE     SARS-CoV Nucleocapsid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SARS-CoV Nucleocapsid
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1629..1763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1653..1672)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1707..1726
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1876..1896)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1876..1894)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1915..1931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1915..1931)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1928..1950)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1939..1955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1963..1969,1970..1987,1988..1993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2008..2029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2146..2163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2317..2905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2397..2416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3076..3867,3868..3936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3699..3718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3937..4041)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4067..4522)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4204..4225)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4416..4435
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4596..5580
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 agcttgggcg acctcaccat gggatccacc atgtctgata atggacccca atcaaaccaa
      361 cgtagtgccc cccgcattac atttggtgga cccacagatt caactgacaa taaccagaat
      421 ggaggacgca atggggcaag gccaaaacag cgccgacccc aaggtttacc caataatact
      481 gcgtcttggt tcacagctct cactcagcat ggcaaggagg aacttagatt ccctcgaggc
      541 cagggcgttc caatcaacac caatagtggt ccagatgacc aaattggcta ctaccgaaga
      601 gctacccgac gagttcgtgg tggtgacggc aaaatgaaag agctcagccc cagatggtac
      661 ttctattacc taggaactgg cccagaagct tcacttccct acggcgctaa caaagaaggc
      721 atcgtatggg ttgcaactga gggagccttg aatacaccca aagaccacat tggcacccgc
      781 aatcctaata acaatgctgc caccgtgcta caacttcctc aaggaacaac attgccaaaa
      841 ggcttctacg cagagggaag cagaggcggc agtcaagcct cttctcgctc ctcatcacgt
      901 agtcgcggta attcaagaaa ttcaactcct ggcagcagta ggggaaattc tcctgctcga
      961 atggctagcg gaggtggtga aactgccctc gcgctattgc tgctagacag attgaaccag
     1021 cttgagagca aagtttctgg taaaggccaa caacaacaag gccaaactgt cactaagaaa
     1081 tctgctgctg aggcatctaa aaagcctcgc caaaaacgta ctgccacaaa acagtacaac
     1141 gtcactcaag catttgggag acgtggtcca gaacaaaccc aaggaaattt cggggaccaa
     1201 gacctaatca gacaaggaac tgattacaaa cattggccgc aaattgcaca atttgctcca
     1261 agtgcctctg cattctttgg aatgtcacgc attggcatgg aagtcacacc ttcgggaaca
     1321 tggctgactt atcatggagc cattaaattg gatgacaaag atccacaatt caaagacaac
     1381 gtcatactgc tgaacaagca cattgacgca tacaaaacat tcccaccaac agagcctaaa
     1441 aaggacaaaa agaaaaagac tgatgaagct cagcctttgc cgcagagaca aaagaagcag
     1501 cccactgtga ctcttcttcc tgcggctgac atggatgatt tctccagaca acttcaaaat
     1561 tccatgagtg gagcttctgc tgattcaact caggcataat ctagaactat agtgagtcgt
     1621 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1681 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1741 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1801 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
     1861 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     1921 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1981 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2041 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2101 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2161 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2221 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2281 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2341 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2401 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2461 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2521 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2581 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2641 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2701 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2761 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2821 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2881 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2941 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3001 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3061 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3121 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3181 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3241 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3301 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3361 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3421 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3481 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3541 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3601 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3661 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3721 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3781 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3841 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3901 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3961 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4021 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     4081 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     4141 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     4201 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     4261 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     4321 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     4381 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     4441 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     4501 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     4561 cgatcggtgc gggcctcttc gctattacgc cagtcgacca tagccaattc aatatggcgt
     4621 atatggactc atgccaattc aatatggtgg atctggacct gtgccaattc aatatggcgt
     4681 atatggactc gtgccaattc aatatggtgg atctggaccc cagccaattc aatatggcgg
     4741 acttggcacc atgccaattc aatatggcgg acttggcact gtgccaactg gggaggggtc
     4801 tacttggcac ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt ccgccatatt
     4861 gaattggcat ggtgccaata atggcggcca tattggctat atgccaggat caatatatag
     4921 gcaatatcca atatggccct atgccaatat ggctattggc caggttcaat actatgtatt
     4981 ggccctatgc catatagtat tccatatatg ggttttccta ttgacgtaga tagcccctcc
     5041 caatgggcgg tcccatatac catatatggg gcttcctaat accgcccata gccactcccc
     5101 cattgacgtc aatggtctct atatatggtc tttcctattg acgtcatatg ggcggtccta
     5161 ttgacgtata tggcgcctcc cccattgacg tcaattacgg taaatggccc gcctggctca
     5221 atgcccattg acgtcaatag gaccacccac cattgacgtc aatgggatgg ctcattgccc
     5281 attcatatcc gttctcacgc cccctattga cgtcaatgac ggtaaatggc ccacttggca
     5341 gtacatcaat atctattaat agtaacttgg caagtacatt actattggaa ggacgccagg
     5401 gtacattggc agtactccca ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa
     5461 gtacatcccc attgacgtca atggggaggg gcaatgacgc aaatgggcgt tccattgacg
     5521 taaatgggcg gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg tttagggaac
//
