LOCUS       sequence-298565-       11717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin 
            resistant) off PGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian cell proliferation requires noncatalytic functions of 
            O-GlcNAc transferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 
            10.1073/pnas.2016778118.
  PUBMED    33419956
REFERENCE   2  (bases 1 to 11717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2016778118. doi"; pages: " 10.1073/pnas.2016778118"
COMMENT     Sequence Label: pLenti PGK FKBP12(F36V)-OGT (Neo)
FEATURES             Location/Qualifiers
     source          1..11717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3708..3752
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4019..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4183..4693
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4612..4630
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4746..4770
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4801..4803,4804..5124)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             5131..5157
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5131..5157
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5161..5187
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5203..5227
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8365..8389)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8420..9008
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8473..8493)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8891..8902)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9051..9550
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9086..9109)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9483..9502
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9564..10367
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9627..9646)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10237..10256
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             10458..10691
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10763..10884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10800..10819)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10854..10873
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10903..11038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11070..11089)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11071..11089)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11099..11116)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11099..11115)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11108..11130)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11260..11715
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11347..11366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11557..11578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc accggtcgtc gactagtcca gtgtggtgga attctgcaga
     4741 tatcaacaag tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
     4801 atgggagtgc aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc
     4861 cagacctgcg tggtgcacta caccgggatg cttgaagatg gaaagaaagt tgattcctcc
     4921 cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg
     4981 gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat
     5041 tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc
     5101 gatgtggagc ttctaaaact ggaaggcggc tacccctacg acgtgcccga ctacgccggc
     5161 tatccgtatg atgtcccgga ctatgcaggc atcgatagat caacaagttt gtacaaaaaa
     5221 gcaggctttg cgtcttccgt gggcaacgtg gccgacagca cagggttagc tgagttggca
     5281 catcgagaat atcaggcagg agattttgag gcagctgaga gacactgcat gcagctctgg
     5341 agacaagagc cagacaatac tggtgtgctt ttattacttt catctataca cttccagtgt
     5401 cgaaggctgg acagatctgc tcactttagc actctggcaa ttaaacagaa cccccttctg
     5461 gcagaagctt attcgaattt ggggaatgtg tacaaggaaa gagggcagtt gcaggaggca
     5521 attgagcatt atcgacatgc attgcgtctc aaacctgatt tcatcgatgg ttatattaac
     5581 ctggcagccg ccttggtagc agcgggtgac atggaagggg cagtacaagc ttacgtctct
     5641 gctcttcagt acaatcctga tttgtactgt gttcgcagtg acctggggaa cctgctcaaa
     5701 gccctgggtc gcttggaaga agccaaggca tgttatttga aagcaattga gacgcaaccg
     5761 aactttgcag tagcttggag taatcttggc tgtgttttca atgcacaagg ggaaatttgg
     5821 cttgcaattc atcactttga aaaggctgtc acccttgacc caaactttct ggatgcttat
     5881 atcaatttag gaaatgtctt gaaagaggca cgcatttttg acagagctgt ggcagcttat
     5941 cttcgtgccc taagtttgag tccaaatcac gcagtggtgc acggcaacct ggcttgtgta
     6001 tactatgagc aaggcctgat agatctggca atagacacct acaggcgggc tatcgaacta
     6061 caaccacatt tccctgatgc ttactgcaac ctagccaatg ctctcaaaga gaagggcagt
     6121 gttgctgaag cagaagattg ttataataca gctctccgtc tgtgtcccac ccatgcagac
     6181 tctctgaata acctagccaa tatcaaacga gaacagggaa acattgaaga ggcagttcgc
     6241 ttgtatcgta aagcattaga agtcttccca gagtttgctg ctgcccattc aaatttagca
     6301 agtgtactgc agcagcaggg aaaactgcag gaagctctga tgcattataa ggaggctatt
     6361 cgaatcagtc ctacctttgc tgatgcctac tctaatatgg gaaacactct aaaggagatg
     6421 caggatgttc agggagcctt gcagtgttat acgcgtgcca tccaaattaa tcctgcattt
     6481 gcagatgcac atagcaatct ggcttccatt cataaggatt cagggaatat tccagaagcc
     6541 atagcttctt accgcacggc tctgaaactt aagcctgatt ttcctgatgc ttattgtaac
     6601 ttggctcatt gcctgcagat tgtctgtgat tggacagact atgatgagcg aatgaagaag
     6661 ttggtcagta ttgtggctga ccagttagag aagaataggt tgccttctgt gcatcctcat
     6721 catagtatgc tatatcctct ttctcatggc ttcaggaagg ctattgctga gaggcacggc
     6781 aacctgtgct tagataagat taatgttctt cataaaccac catatgaaca tccaaaagac
     6841 ttgaagctca gtgatggtcg gctgcgtgta ggatatgtga gttccgactt tgggaatcat
     6901 cctacttctc accttatgca gtctattcca ggcatgcaca atcctgataa atttgaggtg
     6961 ttctgttatg ccctgagccc agacgatggc acaaacttcc gagtgaaggt gatggcagaa
     7021 gccaatcatt tcattgatct ttctcagatt ccatgcaatg gaaaagcagc tgatcgcatc
     7081 catcaggatg gaattcatat ccttgtaaat atgaatggct atactaaggg cgctcgaaat
     7141 gagctttttg ctctcaggcc agctcctatt caggcaatgt ggctgggata ccctgggacg
     7201 agtggtgcgc ttttcatgga ttatattatc actgatcagg aaacttcgcc agctgaagtt
     7261 gctgagcagt attccgagaa attggcttat atgccccaca ctttttttat tggtgatcat
     7321 gctaatatgt tccctcacct gaagaaaaaa gcagtcatcg attttaagtc caatgggcac
     7381 atttatgaca atcggatagt tctgaatggc atcgacctca aagcatttct tgatagtcta
     7441 ccagatgtga aaattgtcaa gatgaagtgt cctgatggag gagacaatgc agatagcagt
     7501 aacacagctc ttaatatgcc tgttattcct atgaatacta ttgcagaagc agttattgaa
     7561 atgattaacc gaggacagat tcaaataaca attaatggat tcagtattag caatggactg
     7621 gcaactactc agatcaacaa taaggctgca actggagagg aggttccccg taccattatt
     7681 gtaaccaccc gttctcagta cgggttacca gaagatgcca tcgtatactg taactttaat
     7741 cagttgtata aaattgaccc ttctactttg cagatgtggg caaacattct gaagcgtgtt
     7801 cccaatagtg tactctggct gttgcgtttt ccagcagtag gagaacctaa tattcaacag
     7861 tatgcacaaa acatgggcct gccccagaac cgtatcattt tttcacctgt tgctcctaaa
     7921 gaggaacacg tcaggagagg ccagctggct gatgtctgct tggacactcc actctgtaat
     7981 gggcacacca cagggatgga tgtcctctgg gcagggaccc ccatggtgac tatgccagga
     8041 gagactcttg cttctcgagt tgcagcatcc cagctcactt gcttaggttg tcttgagctt
     8101 attgctaaaa acagacaaga atatgaagac atagctgtga agctgggaac tgatctagaa
     8161 tacctgaaga aagttcgtgg caaagtctgg aagcaaagaa tatctagccc tctgttcaac
     8221 accaaacaat acacaatgga actagagcgg ctctatctac agatgtggga gcattatgca
     8281 gctggcaaca aacctgacca catgattaag cctgttgaag tcactgagtc agcataataa
     8341 ctcgagatat ctagacccag ctttgttcag ctttcttgta caaagtggtt gatatccagc
     8401 acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     8461 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     8521 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     8581 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8641 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8701 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     8761 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     8821 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8881 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8941 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9001 ctccccgcct ggaattctgc agatatccgg actagtgatc taattctacc gggtagggga
     9061 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     9121 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     9181 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     9241 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     9301 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     9361 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     9421 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     9481 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     9541 gggcctttcg acctgcagcc aatatgggat cggccattga acaagatgga ttgcacgcag
     9601 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     9661 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     9721 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     9781 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     9841 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     9901 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     9961 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
    10021 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
    10081 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
    10141 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
    10201 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
    10261 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
    10321 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaggg gatccgtcga
    10381 gggcccggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
    10441 aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt
    10501 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctgcct
    10561 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10621 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10681 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
    10741 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
    10801 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10861 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
    10921 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    10981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    11041 ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
    11101 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    11161 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    11221 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
    11281 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    11341 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    11401 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    11461 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    11521 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    11581 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    11641 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    11701 taacgtttac aatttcc
//
