LOCUS       sequence-298577-        5384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector encoding non-tagged O-GlcNAc Transferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian cell proliferation requires noncatalytic functions of 
            O-GlcNAc transferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 
            10.1073/pnas.2016778118.
  PUBMED    33419956
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2016778118. doi"; pages: " 10.1073/pnas.2016778118"
COMMENT     Sequence Label: pENTR1A hu_ncOGT WT
FEATURES             Location/Qualifiers
     source          1..5384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3613..3712)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3824..3843)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3835..4644
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3902..3921)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4734..5322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5223..5242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccatg gcgtcttccg tgggcaacgt ggccgacagc acagggttag ctgagttggc
      541 acatcgagaa tatcaggcag gagattttga ggcagctgag agacactgca tgcagctctg
      601 gagacaagag ccagacaata ctggtgtgct tttattactt tcatctatac acttccagtg
      661 tcgaaggctg gacagatctg ctcactttag cactctggca attaaacaga acccccttct
      721 ggcagaagct tattcgaatt tggggaatgt gtacaaggaa agagggcagt tgcaggaggc
      781 aattgagcat tatcgacatg cattgcgtct caaacctgat ttcatcgatg gttatattaa
      841 cctggcagcc gccttggtag cagcgggtga catggaaggg gcagtacaag cttacgtctc
      901 tgctcttcag tacaatcctg atttgtactg tgttcgcagt gacctgggga acctgctcaa
      961 agccctgggt cgcttggaag aagccaaggc atgttatttg aaagcaattg agacgcaacc
     1021 gaactttgca gtagcttgga gtaatcttgg ctgtgttttc aatgcacaag gggaaatttg
     1081 gcttgcaatt catcactttg aaaaggctgt cacccttgac ccaaactttc tggatgctta
     1141 tatcaattta ggaaatgtct tgaaagaggc acgcattttt gacagagctg tggcagctta
     1201 tcttcgtgcc ctaagtttga gtccaaatca cgcagtggtg cacggcaacc tggcttgtgt
     1261 atactatgag caaggcctga tagatctggc aatagacacc tacaggcggg ctatcgaact
     1321 acaaccacat ttccctgatg cttactgcaa cctagccaat gctctcaaag agaagggcag
     1381 tgttgctgaa gcagaagatt gttataatac agctctccgt ctgtgtccca cccatgcaga
     1441 ctctctgaat aacctagcca atatcaaacg agaacaggga aacattgaag aggcagttcg
     1501 cttgtatcgt aaagcattag aagtcttccc agagtttgct gctgcccatt caaatttagc
     1561 aagtgtactg cagcagcagg gaaaactgca ggaagctctg atgcattata aggaggctat
     1621 tcgaatcagt cctacctttg ctgatgccta ctctaatatg ggaaacactc taaaggagat
     1681 gcaggatgtt cagggagcct tgcagtgtta tacgcgtgcc atccaaatta atcctgcatt
     1741 tgcagatgca catagcaatc tggcttccat tcataaggat tcagggaata ttccagaagc
     1801 catagcttct taccgcacgg ctctgaaact taagcctgat tttcctgatg cttattgtaa
     1861 cttggctcat tgcctgcaga ttgtctgtga ttggacagac tatgatgagc gaatgaagaa
     1921 gttggtcagt attgtggctg accagttaga gaagaatagg ttgccttctg tgcatcctca
     1981 tcatagtatg ctatatcctc tttctcatgg cttcaggaag gctattgctg agaggcacgg
     2041 caacctgtgc ttagataaga ttaatgttct tcataaacca ccatatgaac atccaaaaga
     2101 cttgaagctc agtgatggtc ggctgcgtgt aggatatgtg agttccgact ttgggaatca
     2161 tcctacttct caccttatgc agtctattcc aggcatgcac aatcctgata aatttgaggt
     2221 gttctgttat gccctgagcc cagacgatgg cacaaacttc cgagtgaagg tgatggcaga
     2281 agccaatcat ttcattgatc tttctcagat tccatgcaat ggaaaagcag ctgatcgcat
     2341 ccatcaggat ggaattcata tccttgtaaa tatgaatggc tatactaagg gcgctcgaaa
     2401 tgagcttttt gctctcaggc cagctcctat tcaggcaatg tggctgggat accctgggac
     2461 gagtggtgcg cttttcatgg attatattat cactgatcag gaaacttcgc cagctgaagt
     2521 tgctgagcag tattccgaga aattggctta tatgccccac acttttttta ttggtgatca
     2581 tgctaatatg ttccctcacc tgaagaaaaa agcagtcatc gattttaagt ccaatgggca
     2641 catttatgac aatcggatag ttctgaatgg catcgacctc aaagcatttc ttgatagtct
     2701 accagatgtg aaaattgtca agatgaagtg tcctgatgga ggagacaatg cagatagcag
     2761 taacacagct cttaatatgc ctgttattcc tatgaatact attgcagaag cagttattga
     2821 aatgattaac cgaggacaga ttcaaataac aattaatgga ttcagtatta gcaatggact
     2881 ggcaactact cagatcaaca ataaggctgc aactggagag gaggttcccc gtaccattat
     2941 tgtaaccacc cgttctcagt acgggttacc agaagatgcc atcgtatact gtaactttaa
     3001 tcagttgtat aaaattgacc cttctacttt gcagatgtgg gcaaacattc tgaagcgtgt
     3061 tcccaatagt gtactctggc tgttgcgttt tccagcagta ggagaaccta atattcaaca
     3121 gtatgcacaa aacatgggcc tgccccagaa ccgtatcatt ttttcacctg ttgctcctaa
     3181 agaggaacac gtcaggagag gccagctggc tgatgtctgc ttggacactc cactctgtaa
     3241 tgggcacacc acagggatgg atgtcctctg ggcagggacc cccatggtga ctatgccagg
     3301 agagactctt gcttctcgag ttgcagcatc ccagctcact tgcttaggtt gtcttgagct
     3361 tattgctaaa aacagacaag aatatgaaga catagctgtg aagctgggaa ctgatctaga
     3421 atacctgaag aaagttcgtg gcaaagtctg gaagcaaaga atatctagcc ctctgttcaa
     3481 caccaaacaa tacacaatgg aactagagcg gctctatcta cagatgtggg agcattatgc
     3541 agctggcaac aaacctgacc acatgattaa gcctgttgaa gtcactgagt cagcataact
     3601 cgagatatct agacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat
     3661 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     3721 ctgcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     3781 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     3841 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     3901 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     3961 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     4021 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     4081 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     4141 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     4201 gcagtgtccc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     4261 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     4321 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     4381 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     4441 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     4501 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     4561 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     4621 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ttattcagat
     4681 tgggccccgt tccactgagc gtcagacccg gtagaaaaga tcaaaggatc ttcttgagat
     4741 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4801 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4861 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4921 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4981 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5041 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5101 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5161 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5221 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5281 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5341 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
