LOCUS       sequence-298596-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF10 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133684
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             175..195
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             229..306
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             334..354
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    803..924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(814..833)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     868..887
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcagatcaga tctttgtcga tcctaccatc cactcgacac acccgccagc ggccgccacc
       61 atgggctata taaacgtttt cgcttttccg tttacgatat atagtctact cttgtgcaga
      121 atgaattctc gtaactacat agcacaagta gatgtagtta actttaatct cacagaaaac
      181 ctttactttc agtcgggacg gcgaatcccg gggctgataa atccctggaa aaggagatgg
      241 aagaagaact ttattgcagt gtctgcggct aaccggttca aaaagatcag ttcaagcggc
      301 gccctagatt acgacattcc tacgaccgcc tccgagaatc tctatttcca gggtgaattt
      361 ggcttggcac aacatgacga ggccgtcgat aacaagttta ataaagaaca gcagaacgct
      421 ttctatgaga tccttcactt accaaatctg aacgaagagc aacgcaacgc tttcattcag
      481 agcctgaaag acgatccctc gcagtctgca aatctcctgg ccgaagccaa gaagctgaat
      541 gacgcacagg ctccaaaagt tgataacaag tttaacaagg agcagcagaa tgctttctac
      601 gagatattgc accttcctaa tctgaatgag gaacaaagaa atgcatttat ccagtcccta
      661 aaagacgatc catcacagag tgccaacctg cttgcggaag ccaaaaagct caatgatgcc
      721 caggccccga aagtagacgc gaaccatcaa taagctagct tgactgactg agatacagcg
      781 taccttcagc tcacagacat gataagatac attgatgagt ttggacaaac cacaactaga
      841 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      901 attataagct gcaataaaca agttaacaac aacaattgca t
//
