LOCUS       sequence-298600-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 E (envelope).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4252962
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             283..303
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             307..372
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    441..562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(452..471)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     506..525
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      677..723
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      753..889
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gatcagatct ttgtcgatcc taccatccac tcgacacacc cgccagcggc cgccaccatg
       61 tacagctttg tatctgaaga gacaggcact ctcatcgtta attcagtgtt gctgttcctg
      121 gcatttgtgg tcttcctttt agtgacccta gcgatactga cggccctccg gctgtgcgct
      181 tattgttgta acattgtcaa cgttagtctc gtgaagccct cgttctacgt ttattcccgc
      241 gtgaaaaatc taaacagctc ccgtgtgcct gatcttcttg tcgaaaacct ttactttcag
      301 tcgatggatt ataaggacca cgatggagac tacaaagatc atgacattga ttacaaggac
      361 gacgatgaca aataaggatc cgcaggcctc tgctagcttg actgactgag atacagcgta
      421 ccttcagctc acagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      481 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      541 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      601 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtattggccc
      661 atctctatcg gtatcgtagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      721 ttgtgcccct cgggccggat tgctatctac cggcattggc gcagaaaaaa atgcctgatg
      781 cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata cggcttcccc
      841 aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag gtcgtcagct
      901 atcctgcag
//
