LOCUS       sequence-298604-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4252833
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             186..206
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             207..248
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    301..422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(312..331)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     366..385
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      537..583
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      613..749
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 atcagatctt tgtcgatcct accatccact cgacacaccc gccagcggcc gccaccatga
       61 tagaactatc gctcatcgac ttttatctgt gcttcttggc tttcctgctt tttctggtgc
      121 tcattatgct aatcattttt tggttcagtc tggagttaca agatcataat gagacatgtc
      181 acgccgaaaa cctttacttt cagtcgggga aacctatacc caatccactt ctcggcttag
      241 attctaccta agctagcttg actgactgag atacagcgta ccttcagctc acagacatga
      301 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      361 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      421 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      481 tttaaagcaa gtaaaacctc tacaaatgtg gtattggccc atctctatcg gtatcgtagc
      541 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgtgcccct cgggccggat
      601 tgctatctac cggcattggc gcagaaaaaa atgcctgatg cgacgctgcg cgtcttatac
      661 tcccacatat gccagattca gcaacggata cggcttcccc aacttgccca cttccatacg
      721 tgtcctcctt accagaaatt tatccttaag gtcgtcagct atcctgca
//
