LOCUS       sequence-298605-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4252833
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    234..282
                     /note="synthetic polyadenylation signal"
     CDS             complement(309..908)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(392..412)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     889..908
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 agatgtttgc aaaagctagg actcgattat acgttgaccg atcagactat caatatctag
       61 gtttaaacag atcggtacgc tgccgtgacc aggcttgagt acaatatctg acgattggac
      121 ttcctgggtc ccaagctgga ttccgactgg ctgactgcca agcaacgata tggatcgtcg
      181 agactcgcac tgagaggatc gttctcggta caccaggcgc gcctagcgtt acacacacaa
      241 aaaaccaaca cacatccatc ttcgatggat agcgatttta ttatctaaga ctaggcttac
      301 atactagcct aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc
      361 tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg
      421 gaggtctcca ggaaggcggg caccccggcg cgctcggcgg cctccactcc ggggagcacg
      481 acggcgctgc ccagaccctt gccctggtgg tcgggcgaga cgccgacggt ggccaggaac
      541 cacgcgggct ccttgggccg gtgcggcgcc aggaggccct ccatctgttg ctgcgcggcc
      601 agccgggaac cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct
      661 tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc
      721 ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg
      781 atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg
      841 agggtgcgta cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac
      901 tcggtcatgt tgtctaacaa aaaagccaaa aacggcca
//
