LOCUS       sequence-298609-        4272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for Gateway cloning of TMPRSS2 into destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4272)
  TITLE     Gingras Lab Plasmids
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR223 TMPRSS2, closed
FEATURES             Location/Qualifiers
     source          1..4272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(92..178)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     227..249
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     241..258
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     242..258
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    274..373
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1856..1955)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1972..1991)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1973..1991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1996..2012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1996..2012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2443..3234
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3327..3915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3816..3835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4069..4086
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4245..4272)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
       61 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
      121 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
      181 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
      241 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata
      301 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac
      361 aaaaaagcag gcttcatggc tttgaactca gggtcaccac cagctattgg accttactat
      421 gaaaaccatg gataccaacc ggaaaacccc tatcccgcac agcccactgt ggtccccact
      481 gtctacgagg tgcatccggc tcagtactac ccgtcccccg tgccccagta cgccccgagg
      541 gtcctgacgc aggcttccaa ccccgtcgtc tgcacgcagc ccaaatcccc atccgggaca
      601 gtgtgcacct caaagactaa gaaagcactg tgcatcacct tgaccctggg gaccttcctc
      661 gtgggagctg cgctggccgc tggcctactc tggaagttca tgggcagcaa gtgctccaac
      721 tctgggatag agtgcgactc ctcaggtacc tgcatcaacc cctctaactg gtgtgatggc
      781 gtgtcacact gccccggcgg ggaggacgag aatcggtgtg ttcgcctcta cggaccaaac
      841 ttcatccttc aggtgtactc atctcagagg aagtcctggc accctgtgtg ccaagacgac
      901 tggaacgaga actacgggcg ggcggcctgc agggacatgg gctataagaa taatttttac
      961 tctagccaag gaatagtgga tgacagcgga tccaccagct ttatgaaact gaacacaagt
     1021 gccggcaatg tcgatatcta taaaaaactg taccacagtg atgcctgttc ttcaaaagca
     1081 gtggtttctt tacgctgtat agcctgcggg gtcaacttga actcaagccg ccagagcagg
     1141 atcgtgggcg gcgagagcgc gctcccgggg gcctggccct ggcaggtcag cctgcacgtc
     1201 cagaacgtcc acgtgtgcgg aggctccatc atcacccccg agtggatcgt gacagccgcc
     1261 cactgcgtgg aaaaacctct taacaatcca tggcattgga cggcatttgc ggggattttg
     1321 agacaatctt tcatgttcta tggagccgga taccaagtag aaaaagtgat ttctcatcca
     1381 aattatgact ccaagaccaa gaacaatgac attgcgctga tgaagctgca gaagcctctg
     1441 actttcaacg acctagtgaa accagtgtgt ctgcccaacc caggcatgat gctgcagcca
     1501 gaacagctct gctggatttc cgggtggggg gccaccgagg agaaagggaa gacctcagaa
     1561 gtgctgaacg ctgccaaggt gcttctcatt gagacacaga gatgcaacag cagatatgtc
     1621 tatgacaacc tgatcacacc agccatgatc tgtgccggct tcctgcaggg gaacgtcgat
     1681 tcttgccagg gtgacagtgg agggcctctg gtcacttcga agaacaatat ctggtggctg
     1741 ataggggata caagctgggg ttctggctgt gccaaagctt acagaccagg agtgtacggg
     1801 aatgtgatgg tattcacgga ctggatttat cgacaaatga gggcagacgg ctaacaccca
     1861 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1921 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1981 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2041 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     2101 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     2161 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     2221 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     2281 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     2341 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     2401 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
     2461 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     2521 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     2581 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2641 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2701 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2761 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2821 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2881 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2941 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     3001 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     3061 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     3121 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     3181 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     3241 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3301 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3361 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3421 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3481 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3541 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3601 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3661 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3721 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3781 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3841 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3901 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3961 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4021 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4081 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4141 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4201 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4261 ggatggcctt ct
//
