LOCUS       sequence-298642-        9210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-cassette for SapTrap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1886
FEATURES             Location/Qualifiers
     source          1..9210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1538..1571
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             1581..1610
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3427..3750)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5668..5692
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5695..6720
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5695..6720
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6721..6745)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7098..7131
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7156..7176
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             7198..7329
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7357..7422
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(7451..7470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7452..7470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7475..7491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7475..7491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7593..7612)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7604..8413
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7671..7690)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8560..9148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9049..9068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgctc ttcggcgggt ggcggtggat
      601 cgggaggagg aggttcgggt ggcggaggca gtggaggtgg cggaagtggc ggcggtggtt
      661 caggaggagg cggatccatg gtctccaaag gagaagctgt gatcaaggag ttcatgcgct
      721 tcaaggttca catggaagga agcatgaatg gtcacgagtt cgaaatcgaa ggagaagggg
      781 agggccgccc gtacgaggga actcagaccg ccaagctgaa ggtcaccaag ggaggtaagt
      841 ttgtgataat ccaatttcaa ttcgcaatgg tcatcgtttt ttcaggacca cttccattct
      901 catgggatat tctctcccca caattcatgt acggctcccg tgctttcatc aaacacccag
      961 ccgacattcc agattactac aagcaatctt tcccagaagg cttcaagtgg gagcgtgtga
     1021 tgaacttcga ggatggtgga gcagttacag taactcagga cacttctctc gaggtaagtt
     1081 tttactccgc ttttaacaat ggttgtttga catcattttt tcaggatggc actttgatct
     1141 acaaggtcaa gctccgtggt accaatttcc caccagatgg accagttatg cagaagaaga
     1201 cgatgggatg ggaggcttcc accgaacgat tgtacccaga agatggagtt ctcaagggag
     1261 acatcaaaat ggcgttacgc ctcaaggacg gaggtaagtt tgatgaaacg gtttcgtctt
     1321 atatacacta atggtacttt tcaggacgtt acctggcgga tttcaagacc acatacaagg
     1381 caaagaagcc agttcaaatg ccaggagcat ataacgttga ccgcaagctt gatattactt
     1441 cccataatga agactacaca gttgtagaac aatacgaacg atccgaggga cgtcattcga
     1501 ccggaggaat ggacgagctg tacaaggtaa gtttaaaata acttcgtata atgtatacta
     1561 tacgaagtta ttttcaggga gagcaaaagc ttatctccga ggaggacctc taaggatgat
     1621 cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat
     1681 tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa
     1741 tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa
     1801 cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg
     1861 gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa
     1921 caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg
     1981 aggaaggaaa ctagctgccc tcgattgtct atcttccatt ctcaccaagt ttcaacaact
     2041 ttattcagtc attcttctct ggttcatttc ccaaccaatc ttcccgaata aagcccatct
     2101 gtcctttcgg ccacattcct cacctacatc cgtactcctt atctcccgtt cttatccagt
     2161 cttttatcgt ctctgcgtct cgcacactgt ctcgtcggta atgtaaaaaa aaaacaaaaa
     2221 aaacttgaaa acttgcggaa agatttccta ataaaaggag acgaatccgg taggaaatcg
     2281 acaagtctct gcttctcgaa gactaggtga gttacatttc tttggccgaa ttattttgaa
     2341 atcctaaatt tgataatttc agagatgtct gtaaaacttg cgtgttatgt gacggcttcg
     2401 gtcactgtcg ccactcttat ggtatgcttc atgaccatgt caaccatcta ctcggaggtt
     2461 gatggattca gagagaagct tgataccgag atgaatgtgt tcagagtgag ttaaattata
     2521 ttttgaattt taaataattt ttaattttct agcaatctac caatggattg tggaaggaca
     2581 tagttgtcat cggaagatct agcaagcgtg tccgttgtca atatgaagag accaacgcta
     2641 ccccaactcc acatgctgat ggatccccat ctgctccacc aggtcaacca ccagcagttc
     2701 caccagtctt caaccagcca aagactccaa atggagccaa tggaaatgga ccaacctgca
     2761 actgcaatgc tgataacaag tgcccagctg gaccatccgg accaaaggga gttccaggag
     2821 ttccaggact cgacggagtt ccaggacttg acggtgttcc aggagttgga gctgatgata
     2881 tcgctccaca acgcgagtct gtcggatgct tcacttgccc acaaggacca gttggaccac
     2941 caggagctct tggaagacca ggaccacgtg gacttccagg accaagagga caaaatggaa
     3001 acccaggaag agatggacaa ccaggacatc caggagagca aggatcatcc ggtcaaatcg
     3061 gaaagatcgg agagccagga ccaccaggag agaagggacg cgacgccgag catccaatcg
     3121 gaagaccagg accaaaggga ccaagaggag atcaaggacc aacaggacca gctggacaga
     3181 acggtcttca cggaccacca ggagagccag gaaccgttgg accagaagga ccatctggaa
     3241 agcaaggacg tcaaggacca gacggaaccc agggagagac tggaccagac ggaagaccag
     3301 gaaaggatgc cgagtactgc cagtgcccag acaagtctcc accatcagag gctgtcaacg
     3361 ccaaccgtgg atacagaaat atctaaattg ttggtgtttt ctaataaaat atttgagatt
     3421 cagtgagact tttttcttgg cggcacaata aagtttcctc tttttgttgg gtcactcaac
     3481 gaacacaata caaatatttg agcgctcgca tttttctcaa cctttcgggt ttaaagcact
     3541 tcaaaatgga attgaagaga gattgtgaaa gtacatcaga cagaatcaca ttttgatagc
     3601 attcacttca ctcagatgca aacttttcag tgactataaa aagtcataaa ataataacat
     3661 taaaaaagca aataaattag cgagagaatt ttttgacaaa aagaaagaag agtgatagag
     3721 aagaagggaa tgcttgaaag gattttgcat ttatcttaat caccgtcttc gagaagacga
     3781 accatggcgc cagtttcaga atccaagttt ctaatatagt tcatcacaat gttgacgttg
     3841 gtccaacctc cggcttgcat aatctctggg atggaaactc cagcgcgagc catgtcacgg
     3901 gcagctccga cacgggcgga atgtccagac caagccaagt aacgttgtcc ggaatcatcc
     3961 ttggctccgt agatgagacg gtgggtagct tcgaagattc ctgaaaattt attttcagct
     4021 tattcttagc taatatgttg tttaaactta ccttcaagag ctcgagtcga gagttgtgat
     4081 gttgcactcg gggcagcaac tccgttcttg cgaacacgac agaacaggta gttgtttgga
     4141 tcatcggcga ctccggaaac cgaaatccag cgctcaacga gcttggtaac tccaagggag
     4201 agcgcttttt cgactccggc agtcgagacc agcgtttttg tacgtccgat gtggatgagc
     4261 attcttcctc cgtctgtgcg actaatatcc ttgacgcgaa tgcgggcaat ttcggcgata
     4321 cggagaagtg tgttgtaagc aattccgagg aaggcgaggt ttcggatgtc ctggcaacga
     4381 tcggaatttt ccataagaga acggacctga tcgaagtcgg ttctgaaaat ttcgtcaagt
     4441 cttaggattg aatacattaa tatttaaact tacctttcga aggcgagggc ttgcttggct
     4501 ctctctccag catcaacatt ttccttgcgg atacgtctca ttacgagaga tacggcatta
     4561 gaatctgatg ggcgtggaag tccagatcgg cgatggagca tattgagctg tccgagatgt
     4621 tgttggatcg tcttcaccgc caatccacga gcttggaggt agagcaagta gtcacgaacg
     4681 tcttctggct cggctgggaa ccacttgcgg ttgttaagct tgcaccaggc agcccaagaa
     4741 cggcacactg agagaagcat cttccaggtg tgctcggaga aggcttgtct gtcacggaac
     4801 atatccatga ggttcttgcg aacttcatca cttgtcgcgt caactggaag tgctggaaga
     4861 ttttgatgaa cagtgagaag atttgacata ttttcgaagt tttttagatg cactagaaca
     4921 aagcgtgttg gcttcctctg agcccgcttt ccttatatac ccgcattctg cagccttaca
     4981 gaatgttcta gaaggtccta gatgcattcg tttgaaaata ctcccggtgg gtgcaaagag
     5041 acgcagacgg aaaatgtatc tgggtctctt tattgtgtac actacttttc catgtaccga
     5101 atgtgagtcg ccctcctttt gcaacaagca gctcgaatgt tctagaaaaa ggtggaaaat
     5161 agtataaata ccgttgaaaa taaataccga acaacatttg ctctaattgt gaaattagaa
     5221 atcttcaaac tataatcatg tcactttacc actatttccg tccagctcaa cgttccgttt
     5281 ttggtgatca tttttgcttt cgtcgtaaat ctacacacgc gtctcttccg tgcgagagtc
     5341 caagccagca gccaaattcg ttgactgagt attcaacgtt tatacgttgt cggcaacgag
     5401 aaataggaaa atgcatcggg aaatgttctt ttttcgattt tttccaaggt tttgacaaat
     5461 tttaccacga attttgctat gttttcaatt aaaaaatatg ttattcaact gtttctatga
     5521 ggaaaataag gctttgcatg taattttctt attcagcata atttttaatt aatttgaatt
     5581 ttctgtccta acgtttattt tgttttcttg gttatgactg atctgaaatt aatttttgaa
     5641 ttttaaggta atatgtcagg cggtgccgca agtttgtaca aaaaagcagg ctccatgaaa
     5701 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     5761 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     5821 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     5881 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     5941 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     6001 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     6061 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     6121 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     6181 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     6241 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     6301 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     6361 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     6421 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     6481 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     6541 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     6601 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     6661 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     6721 acccagcttt cttgtacaaa gtgggtccaa ttactcttca acatccctac atgctctttc
     6781 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     6841 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     6901 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     6961 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     7021 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     7081 tatgaccgca atttttaata acttcgtata atgtatacta tacgaagtta ttttcagggt
     7141 ggcggcagcg gaggagagaa tctgtacttt caatccggat ccggaggtgg cgggatgcct
     7201 aaagatccag ccaaacctcc ggccaaggca caagttgtgg gatggccacc ggtgagatca
     7261 taccggaaga acgtgatggt ttcctgccaa aaatcaagcg gtggcccgga ggcggcggcg
     7321 ttcgtgaagg gtggtggcgg ttctggctca ggatctgact acaaagacca tgacggtgat
     7381 tataaagatc atgatatcga ttacaaggat gacgatgaca agacgcgaag agcgccatcc
     7441 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     7501 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac
     7561 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     7621 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     7681 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     7741 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     7801 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     7861 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     7921 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     7981 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     8041 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     8101 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     8161 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     8221 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     8281 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     8341 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     8401 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     8461 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     8521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8701 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9181 ggccttttgc tggccttttg ctcacatgtt
//
