LOCUS       sequence-298649-        4025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1948
FEATURES             Location/Qualifiers
     source          1..4025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             882..902
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1922..2245
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2266..2285)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2267..2285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2290..2306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2290..2306)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2408..2427)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2419..3228
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2486..2505)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3375..3963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3864..3883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatc gattttttgc aaattacgag
      601 cgttgtaggg ggcggagcga taggtcctat aggttttggt atatcatcat tcattcattc
      661 attggtacat tcatttaccc accttcctct ttctgagctt ctctggagtt ctgtgcttcc
      721 tttttccctt atctttatac tgtaattttt aactttcagg cattgattgg atccccggga
      781 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
      841 ggaggaccct tgcttggagg gtaccgagct cagaaaaaat gccaaagaag aagcgtaagg
      901 tcctgcagat ggtctccaag ggagaggctg tcatcaagga gttcatgcgt ttcaaggtcc
      961 acatggaggg atccatgaac ggacacgagt tcgagatcga gggagaggga gagggacgtc
     1021 catacgaggg aacccaaacc gccaagctca aggtcaccaa gggaggtaag tttgtgataa
     1081 tccaatttca attcgcaatg gtcatcgttt tttcaggacc actcccattc tcctgggaca
     1141 tcctctcccc acaattcatg tacggatccc gtgccttcat caagcaccca gccgacatcc
     1201 cagactacta caagcaatcc ttcccagagg gattcaagtg ggagcgtgtg atgaacttcg
     1261 aggtaagttt ttactccgct tttaacaatg gttgtttgac atcatttttt caggacggag
     1321 gagccgtcac cgtcacccaa gacacctccc tcgaggacgg aaccctcatc tacaaggtca
     1381 agctccgtgg aaccaacttc ccaccagacg gaccagtcat gcaaaagaag accatgggat
     1441 gggaggcctc caccgagcgt ctctacccag aggacggagt cctcaaaggt aagtttgatg
     1501 aaacggtttc gtcttatata cactaatggt acttttcagg agacatcaag atggccctcc
     1561 gtctcaagga cggaggacgt tacctcgccg acttcaagac cacctacaag gccaagaagc
     1621 cagtccaaat gccaggagcc tacaacgtcg accgtaagct cgacatcacc tcccacaacg
     1681 aggactacac cgtcgtcgag caatacgagc gttccgaggg acgtcactcc accggaggaa
     1741 tggacgagct ctacaagtcc ggaggatccg gaggaaccgg aggatcctct ctttctcacg
     1801 gattcccacc agctgttgct gctcaagatg atggaaccct tccaatgtct tgcgctcaag
     1861 agtctggaat ggatcgtcac ccagctgctt gcgcttccgc tcgtatcaac gtctaagata
     1921 aatgcaaaat cctttcaagc attcccttct tctctatcac tcttctttct ttttgtcaaa
     1981 aaattctctc gctaatttat ttgctttttt aatgttatta ttttatgact ttttatagtc
     2041 actgaaaagt ttgcatctga gtgaagtgaa tgctatcaaa atgtgattct gtctgatgta
     2101 ctttcacaat ctctcttcaa ttccattttg aagtgcttta aacccgaaag gttgagaaaa
     2161 atgcgagcgc tcaaatattt gtattgtgtt cgttgagtga cccaacaaaa agaggaaact
     2221 ttattgtgcc gccaagaaaa aagtctcagc catccagctg atatccccta tagtgagtcg
     2281 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2341 tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc ttacataaac
     2401 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2461 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2521 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2581 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2641 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2701 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2761 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2821 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2881 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2941 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     3001 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     3061 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     3121 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3181 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3241 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3301 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3361 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3421 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3481 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3541 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3601 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3661 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3721 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3781 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3901 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3961 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4021 atgtt
//
