LOCUS       sequence-298680-       12335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse IFT88-EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12335)
  TITLE     lentiviral expression plasmids for ciliary genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-mIFT88-EGFP
FEATURES             Location/Qualifiers
     source          1..12335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2367..3545
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2598..3536
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3493..3513
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3563..3572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6062..6064,6065..6067,6068..6781)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6062..6064,6065..6067,6068..6778)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6107..6128)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6368..6387)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6715..6736
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6791..7379
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6844..6864)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7262..7273)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7903..8083
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8109..8126)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8115..8339
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8385..8813
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8472..8491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8682..8703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8822..8842)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8827..9156
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9007..9142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9069..9088
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9204..9251
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9270..9644
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9774..9895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9811..9830)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9865..9884
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9944..9960)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9944..9960)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9957..9979)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9968..9984
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9992..9998,9999..10016,10017..10022))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10037..10058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10175..10192)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10346..10934)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10426..10445)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11105..11896,11897..11965))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11728..11747
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11966..12070)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12145..12164)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaattggct ccggtgcccg tcagtgggca gagcgcacat
     2401 cgcccacagt ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa
     2461 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2521 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2581 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     2641 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     2701 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     2761 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     2821 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     2881 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     2941 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     3001 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3061 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3121 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3181 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3241 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3301 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3361 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     3421 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     3481 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     3541 cgtgaggaat tgggccatta cggccaccat ggaaaatgtt catctggcac cagaaacaga
     3601 tgaggacgac ctttactctg gtttcaacga ctacaaccca gcctatgata ctgaggagtt
     3661 ggagaatgac acgggttttc agcaagcagt gagaaccagt catggcagaa gacctcccgt
     3721 aactgctaaa ataccaagca cagcagtcag cagacccata gctactggat atgggtccaa
     3781 gacatccctg acatcatcaa tgggaagacc gatgacaggg acaattcagg atggagttgc
     3841 tcgaccgatg acagcagtga gagcagctgg cttttccaag gcagctttgc gaggctctgc
     3901 atttgacccc cttggtcagt ccaggggccc tgctcctcct ttggaagcca aaaatgaaga
     3961 cagtccagaa gaaaaaatta gacagttgga gaagaaagta aatgagttgg tggaagaaag
     4021 ctgtattgcc aacagttgtg gagacttaaa actggcctta gagaaggcaa aagatgcagg
     4081 aagaaaagag agagtcctgg tgagacaacg agagcaagtc acaagtccag aaaatatcaa
     4141 cttggactta acctactccg ttcttttcaa cttggccagt cagtattctg ctaatgaaat
     4201 gtatgcagaa gcactgaaca cataccaagt tatagtgaaa aacaagatgt ttagcaatgc
     4261 aggaagactg aaagtgaata tgggaaatat ttatttaaag cagagaaatt attccaaagc
     4321 cattaagttc taccgaatgg ccttagatca gatcccaagt gtccataaag aaatgaggat
     4381 caaaataatg cagaacattg gaatcacatt tataaaaact ggtcagtact cagatgccat
     4441 caactcattt gagcacatca tgagcatggc cccgagcctg aaggcaggct tcaacctcat
     4501 ccttagttgt tttgccattg gagatcggga gaaaatgaag aaggcattcc aaaaattaat
     4561 tgctgttccg ttagaaattg atgaagatga taaatatatc tccccaagtg atgatcccca
     4621 tactaacttg ctgattgaag ctataaaaaa tgaccacctt aggcaaatgg aacgtgaaag
     4681 gaaagccatg gcagaaaaat acatcatgac agctgcaaaa ctcattgctc ctgtgatcga
     4741 agcgtctttt gctgtgggtt ataactggtg tgtggaagta gtgaaagcct ctcagtatgt
     4801 ggagctggcc aacgacctgg agattaacaa agcaattact tacttgagac aaaaggactt
     4861 taaccaagct gtagacacat tgaaaatgtt tgagaagaag gacagtagag tgaagagtgc
     4921 agctgcgacc aacctctcgt tcctgtatta tctggaaaac gaatttgccc aagccagcag
     4981 ctatgcagat ttagctgtga actcggatag atataacccg tcagctctca ctaataaagg
     5041 gaacacagtt tttgcaaatg gtgattatga gaaggcagct gagttctata aagaggccct
     5101 gagaaacgac tcttcctgta ctgaagcact gtataacatt ggcctcacct ataagaagct
     5161 gaaccgtctg gatgaagccc tggactcctt cctgaaactg cacgcgatcc ttcggaacag
     5221 cgctcaagtt ctttgccaga tagcaaacat atatgagtta atggaagatc ccaatcaagc
     5281 tattgaatgg ctgatgcagt tgatcagcgt tgttccaact gattcccaag ctctgtctaa
     5341 actgggagag ttatacgata gtgagggaga caagtcccag gcattccaat attactatga
     5401 gtcatacagg tatttccctt ctaacattga agtcattgaa tggcttggag cttattacat
     5461 tgatacacag ttctgcgaga aagccattca gtactttgaa agagcttccc ttatacaacc
     5521 cactcaagtg aagtggcagc tgatggtagc tagctgcttt agaagaagtg gtaactacca
     5581 aaaggcatta gatacttata aagagattca caggaaattt ccggagaatg ttgaatgttt
     5641 gcgtttcttg gttcgtctct gcacagatat tggattaaaa gaagttcaag aatatgccac
     5701 aaaactcaag aggttggaaa aaatgaaaga aatgagggaa cagcgcataa aatcgggcag
     5761 agacagcagc gggggttctc gcagcaaacg agaggggagt gctggcagtg acagtggcca
     5821 gaacaatagt gccagtagta agagtgaacg actgagtgcc aagctcagag ctttgcctgg
     5881 gacagatgaa ccttatgaaa gtagcggtaa caaagaaata gatgcctcct acgtggatcc
     5941 actgggccct cagatagaaa gaccaaaaac tgcagccaag aaaagaattg acgaggatga
     6001 ttttgctgat gaagagttag gagatgactt gctctcagaa ggccgcctcg gccccacaac
     6061 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     6121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     6181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     6241 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     6301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     6361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     6421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     6481 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     6541 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     6601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     6661 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     6721 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     6781 aagcggccgc aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6841 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6901 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6961 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7021 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7081 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7141 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7201 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7261 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7321 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     7381 tgcctgcagg tttgtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     7441 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     7501 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7561 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     7621 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     7681 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     7741 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     7801 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     7861 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     7921 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7981 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8041 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     8101 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     8161 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     8221 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     8281 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     8341 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     8401 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     8461 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8521 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8581 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8641 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8701 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8761 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     8821 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8881 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8941 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     9001 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9061 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     9121 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     9181 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     9241 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     9301 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     9361 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     9421 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     9481 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     9541 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     9601 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     9661 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     9721 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     9781 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9841 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9901 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     9961 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    10021 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    10081 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    10141 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    10201 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10261 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10321 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10381 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10441 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10501 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10561 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10621 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10681 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10741 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10801 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10861 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10921 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10981 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    11041 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    11101 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    11161 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    11221 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    11281 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    11341 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    11401 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    11461 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11521 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11581 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11641 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11701 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11761 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11821 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11881 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11941 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    12001 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    12061 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
    12121 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    12181 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
    12241 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
    12301 gctgcttcgc gatgtacggg ccagatatac gcgtt
//
