LOCUS       sequence-298716-        3520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression of Nup124.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Jiang Z, Jin X, Li Y, Liu S, Liu XM, Wang YY, Zhao P, Cai X, Liu Y, 
            Tang Y, Sun X, Liu Y, Hu Y, Li M, Cai G, Qi X, Chen S, Du LL, He W
  TITLE     Genetically encoded tags for direct synthesis of EM-visible gold 
            nanoparticles in cells.
  JOURNAL   Nat Methods. 2020 Sep;17(9):937-946. doi: 10.1038/s41592-020-0911-z.
            Epub 2020 Aug 10.
  PUBMED    32778831
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0911-z"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "937-946"
COMMENT     Sequence Label: pDUAL-P41nmt1-Nup124-GFP-MTn
FEATURES             Location/Qualifiers
     source          1..3520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(522..533)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgcctcctg tttcaaaaaa tactcgcact tcatcaaaga ctgttaagaa accctatgac
       61 ccacctcagg ggagctcaag gccctttttt acggtcttga aacgggcatt ttcatctgtt
      121 ttgcacccat tcaccagtgg tctagacgag aaagcttcag gaacggctag caaggataga
      181 aagagtggaa gagcaggtac aaaaagcctg cttactccag agttaacacc tcattattta
      241 ggaaaatctc cgtaagttga catttttgtc gtgaaacata ttaacattaa atagaagaat
      301 tattcgcgtt agtaatcgga gccatgttag aacaattgat ggaatagagg aaaaagttca
      361 tacgaataca tttgaaccta ggaagccaaa gcaaaagcaa gattatacta attccccaac
      421 gctttttaaa cgccatgacg agttgtcgtt aaaatcactt aattcgcttc atccttcttc
      481 tgctttatcc aaaaagttgg gcagcacttc tcaacatcaa attgcaactc ccaaatcctc
      541 tgcttcattg ctcaatatcc ttcggtcact tcacgatgag cagaaaaata ccttaaatat
      601 atcaagtgta aaacaagatc gtattaccga agctaatcct acttgtgaga agcgcaagcc
      661 ttctagaagt cccagtccta tgctttccaa aaagaaatct gttgctcgtg cctctgaaaa
      721 cgagccctca gctaagcaga acaaaagctt ttctggaaat gattcccata agagtcttac
      781 cgacattaga gataaggaga atggcgaaac agaggtctca gcaaaaaatc acgttcctca
      841 ccgctcctct cggcgacgtc gtagacacca gcggcttatc cctatcattt atgaaactct
      901 tgaacagatg gatttaagga agcctgtatt agtcaatgca gaagttcaga cagattccaa
      961 ccccggtaat acaatgttta ttgataagca agatatttat caccggttaa gtacacccac
     1021 ttcgcgtaag cgtcagactc tcgagaaagg tcatattaag gctttttctg cagttgatga
     1081 ggatttggat gaaatctttg cttgcgaaga tgacgttcat tacaccgcat tacccaaaca
     1141 aaatccaaaa tctgagcgta tattagaacc tataatagca tctcccaagg ataatacaag
     1201 tgacaagggt ttattgacga agtccgcccc tacctttgaa gagcttcaag cctctattac
     1261 ccctaagcct gtaaagactt ctccgaatga tacggcactt actctcgcga acgctgagga
     1321 taataaaacc tttgaacatc agccattgtc aaaggataca gaagcgccca aaagccaatt
     1381 ttcctcatcg ccgacaaaag aatctacaac tagaaagtcc gaagttgaac cgccatcacc
     1441 ttctaaggaa atcaaatctt ctcacttttc agtacccgaa tttaagtttg aaccgaagac
     1501 tgaagctacc acggacaaaa aattaaatgt acccaaattt gagtttaagc caactgctac
     1561 tgctgatgtg caaactaatc gtttgaagga gaatgagccc aagccaacgt tctttgcaca
     1621 attaccgtca aagactcaag agacgccaag tataactgag aacaagcctt catttttttc
     1681 tcaattaagt ccaaaacgag aagaaacaga aaagaaggac aacgctccgt ctgctcctgc
     1741 ttcaacttct ggtttcagct ttggaggatt cgcacctaaa acccttgaag agaaggaaga
     1801 aacgaaagct cctacgttca atttctcttt aaacaatgct tctagtacac aagacacaac
     1861 aaaacctacg ctacaattta attttggaag tagttttgga aagccaacaa gcaatatttt
     1921 caatgacaaa aaaacttctg aaaatggtct agctagttct actgttgctt ctgagtcaaa
     1981 accgtcagct cccgaatcaa aaccgtcctc gggttttgga aataccgcag gctcatcacc
     2041 cttttcattc aatttgacca aggagtccaa agaagtccca cctacaaatt ccttttcttt
     2101 tgctaaaaaa gggaaagatg aagcgaatga cagtttgtca gctaaagctt ctactccatt
     2161 ttcgtttgca aaacctaata ctgaaaatgt aacaacaact gctcctcagt tttcatttaa
     2221 ttttaccaag ccaaatacgg atgccaaaac caatttgcta cctgaaaaaa cttttaatga
     2281 agaagccgta aagcaaaagg aaactgaaaa agaggtccct ccaacaggtc caaaagcttc
     2341 tgaaataaag gattcagtat cttctaataa cgctgtgccc tcttcaacct ttaattttgt
     2401 atctcccttt gctgctgtat ctgaaaaaac aaatgagaat aacattccta acgatacaac
     2461 gaagacgaat ggcaatgcta ctaagagaac tttggaacaa acggaggatg caaagccttt
     2521 tgctttctcc ttcggttcca ctacagaaca ggcaaacaaa aaagcctcaa cgtcaaatga
     2581 aactacgaag cctcaattgg atacatcttc taaaactgat ggagtaactg ctaatgcgcc
     2641 gtttagtttt gcatcagctt ttaatgcacc taaaccctcg actaatactg ctgatggcaa
     2701 agattctgct tcgaatctta ccactccttc acctgctttt tcatttggaa acaatagtgg
     2761 tgttaaagca tccagtaaca acaatccctc tacaaattcg tctacggcac cgttttcttt
     2821 tggtacatca aataaaccgg catttagctt tgggtctgca acttcaaaga ctacctctga
     2881 aggaaccgct ccagctgctt ctgcttctgc tcctgctccc actacgtctg cattttcttt
     2941 tggagcatca aattctagta tgaataagga agagaacact cccatggcta aggatgctgg
     3001 agatacggca cctgcgtctg gctttaagtc tgggttttcg ttcggtgcta acaattcgcc
     3061 tcagcctgct tctatgtttg gtacatcgac gccggccccg tcttccgcgt ttgcgtttgg
     3121 caaccagtca ggcacgaacc cagcagctcc tgcagggttt ggagggatta ctaatactgc
     3181 cactaataac cctcctagta caggttttac attcactcca tcaaatgctg gatcaactgc
     3241 ggctcctatg tttggcgctg gaaacactcc caacccatcc gggtccatca ataatgcatc
     3301 acaagcattc gccttcgggt caggagaacc tagtaatcca gctagtaatc cacctagtac
     3361 cggattttcg ttcggtgcgg ctactccgtc tgcctttaat gcaagtgcct cacaatcacc
     3421 agcgcccaat ggtattcaat tcaatttggg ctcttctaac tcacaaacaa atgcgccccc
     3481 gggccgtaaa attgctgtgc cccgaagtcg aagaaaacgt
//
