LOCUS       sequence-298775-        5555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bait vector AT2G24230 X040_pECIA2 should be used with prey vector 
            AT2G24230 X040_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: AT2G24230 X040_pECIA2
FEATURES             Location/Qualifiers
     source          1..5555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             1808..1831
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2537..2578
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2588..2605
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2628..2645)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2678..2812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2702..2721)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2756..2775
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2956..2972)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2956..2972)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2969..2991)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2980..2996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3004..3010,3011..3028,3029..3034))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3049..3070
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3187..3204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3358..3946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3438..3457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4117..4908,4909..4977))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4740..4759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4978..5082)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5150..5168
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5206..5228)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5328..5347
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(5541..5555,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg aacacagatg gtttctttgt
      541 ctctgaattc tataagcaaa tgggtttaag ctcttctcaa gcttataact tctctgctcc
      601 tttttgttca tggcaaggtt tgttctgtga ttccaagaat gagcatgtga ttatgttaat
      661 tgcttctgga atgagtttat ctggtcaaat ccctgataac acaattggta aactcagcaa
      721 gcttcaatct ttggatctta gcaacaacaa aatctctgct ttaccatctg atttctggag
      781 tttgaacact ttgaagaatc tcaacctctc tttcaacaag atctctgggt ctttctctag
      841 taatgttggc aattttgggc agcttgagtt acttgatatc tcttacaaca atttctctgg
      901 tgcaatccct gaagctgtgg attctcttgt tagtttgaga gtcttgaagc ttgatcataa
      961 tgggtttcag atgtcgattc cacgaggact tcttggttgc caatccctgg tttctattga
     1021 tctctcctct aaccagcttg aagggtctct tcctgatggg tttggctctg catttccaaa
     1081 gcttgagact ttgagccttg caggaaacaa gattcatggc agggacacag attttgctga
     1141 tatgaaatcc atcagttttc tcaacatttc aggaaatcag tttgatggtt cagtgacagg
     1201 tgtgttcaag gagactttag aagtggctga tctcagcaaa aaccggtttc aaggtcatat
     1261 ctcatctcag gtagactcca actggtttag tttggtttat cttgacttgt ctgagaatga
     1321 gctcagtgga gttatcaaga atctgacact actaaagaag ctcaagcact taaatctagc
     1381 ttggaataga ttcaacagag gaatgtttcc gaggattgag atgctttctg gtttagagta
     1441 tcttaacttg tcaaacacaa atctttctgg tcacatacct agagagatct caaagctgag
     1501 tgatttgagt acactcgatg tttctggaaa ccatcttgca ggacatatcc cgattttgag
     1561 tatcaagaac cttgtggcta tcgatgtgtc gaggaacaat ttgacgggag agataccgat
     1621 gtctatcctt gagaagctcc cttggatgga gagattcaac ttctctttca acaacttaac
     1681 attttgttcc ggaaagttct cagctgaaac tctgaacaga tccttctttg gttctacaaa
     1741 tagctgccct atcgctgcaa acccagcact tttcaagaga aaaagatcag tcactggagg
     1801 acttaagctg gaggtgctgt tccagggacc agggagcgac aaaactcaca catgcccacc
     1861 gtgcccagca cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa
     1921 ggacaccctc atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca
     1981 cgaagaccct gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa
     2041 gacaaagccg cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt
     2101 cctgcaccag gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct
     2161 cccagccccc atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt
     2221 gtacaccctg cccccatccc gggaggagat gaccaagaac caggtcagcc tgacctgcct
     2281 ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga
     2341 gaacaactac aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag
     2401 caagctcacc gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat
     2461 gcatgagggt ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaatc
     2521 tagagggccc ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     2581 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
     2641 ttctaaggcc tgagctcgct gatcagcctc gatcgaggat ccagacatga taagatacat
     2701 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2761 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2821 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     2881 gtaaaacctc tacaaatgtg gtatggctga ttatgatcag tcgacctgca ggcatgcaag
     2941 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3001 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3061 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3121 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5101 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     5161 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     5221 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     5281 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     5341 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     5401 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     5461 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     5521 ttgggtaacg ccagggtttt cccagtcacg acgtt
//
