LOCUS       sequence-298781-        6257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bait vector PSKR2 nC10_pECIA2 should be used with prey vector PSKR2 
            nC10_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: PSKR2 nC10_pECIA2
FEATURES             Location/Qualifiers
     source          1..6257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             2510..2533
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3239..3280
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3290..3307
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3330..3347)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3380..3514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3404..3423)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3458..3477
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3658..3674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3658..3674)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3671..3693)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3682..3698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3706..3712,3713..3730,3731..3736))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3751..3772
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3889..3906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4060..4648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4140..4159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4819..5610,5611..5679))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5442..5461
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5680..5784)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5852..5870
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5908..5930)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6030..6049
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(6243..6257,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg agccaaccat gtcatcccaa
      541 cgacttatct gcgctccggg aattggcagg agcgttgaag aacaagtctg ttacagaatc
      601 ttggttaaat ggttcacgtt gttgtgaatg ggatggtgtg ttttgtgaag ggagtgatgt
      661 ttctggtcga gttacaaagt tggttttacc tgaaaaaggt ttggaaggtg tgatttcgaa
      721 gtctttaggg gagttgactg agctgcgagt acttgatcta tctcgtaacc agcttaaagg
      781 cgaagtacca gcggagattt ctaagttaga gcagcttcaa gttcttgatt tgagtcataa
      841 cctgttatca gggtctgttt tgggagtggt ttcgggttta aagctgattc agtcgctgaa
      901 catttcgagc aattcgctta gcgggaagtt atcggatgtt ggagtgtttc ctggtcttgt
      961 gatgcttaat gtaagcaaca atttgtttga gggtgagatt catcctgaac tctgtagctc
     1021 atctggtggg atacaggttc ttgatttatc gatgaatcgt ttggtgggga atcttgatgg
     1081 cttgtacaac tgcagcaaat ctattcaaca gctccatatc gacagcaaca gattgacggg
     1141 ccaacttccg gattatcttt attcgatccg ggagttggag caactatcac tctctggaaa
     1201 ctacttatcc ggagagttaa gcaagaactt gagcaatctc tctggtctga agtctctgtt
     1261 gatatcagag aaccggtttt cggatgtaat tccagatgtt tttggtaacc tcactcaatt
     1321 ggaacacctc gacgtgagct ccaacaagtt ctcgggaagg tttccgccaa gtttatccca
     1381 atgctcgaag ctgcgggttc ttgatcttag gaacaactcg ttatccggtt ctatcaatct
     1441 taacttcact ggatttaccg atctttgcgt gcttgatctc gccagtaatc atttctctgg
     1501 acctcttcct gattcccttg gccactgtcc caagatgaag atcttgagtt tggcgaaaaa
     1561 cgagtttcgc ggcaaaatcc ctgacacctt caagaatctg cagtctctct tgttcctgtc
     1621 cttatccaac aacagctttg tggatttttc tgagacaatg aatgtgctgc aacattgcag
     1681 aaacctctcc actcttattc tctcaaagaa cttcatcggc gaggaaatac caaacaacgt
     1741 cactggtttc gacaacctcg cgattttagc gctaggaaat tgcggtctta gaggtcagat
     1801 tccgagctgg ctattgaact gcaagaagct ggaagttctt gatctctctt ggaatcactt
     1861 ttacggaact atccctcatt ggattggtaa gatggagagt ttgttctaca tagacttctc
     1921 aaacaacact ttgaccggag caatcccggt agccataacc gagctcaaga acctaatccg
     1981 tctaaacgga accgcttctc agatgaccga ctcttctgga attcctctct acgtaaagcg
     2041 gaacaagagc tccaacggtc ttccatataa ccaagtttca agattcccgc catctatcta
     2101 tttgaataat aaccgtctca acgggacgat cttgccagag ataggacgtt tgaaagagct
     2161 tcacatgctg gacttgagca ggaacaactt cactgggacg atacctgatt ccatttcagg
     2221 gcttgacaat ttggaggttc ttgatttatc ttacaatcat ctctacggtt cgattcctct
     2281 gtcttttcag agtctcactt tcttgtcgag gttcagcgta gcgtataacc gtctcactgg
     2341 cgcgattcca tctggaggtc agttctacag cttcccgcac tcaagcttcg aaggaaactt
     2401 aggactttgt cgcgcgattg attctccttg cgatgttctg atgagtaaca tgttgaatcc
     2461 gaaaggttct tcgcgtagga ataacaatgg cggaaagttc gggagaagcc tggaggtgct
     2521 gttccaggga ccagggagcg acaaaactca cacatgccca ccgtgcccag cacctgaact
     2581 cctgggggga ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc
     2641 ccggacccct gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa
     2701 gttcaactgg tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga
     2761 gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct
     2821 gaatggcaag gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa
     2881 aaccatctcc aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc
     2941 ccgggaggag atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc
     3001 cagcgacatc gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac
     3061 gcctcccgtg ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa
     3121 gagcaggtgg cagcagggga acgtcttctc atgctccgtg atgcatgagg gtctgcacaa
     3181 ccactacacg cagaagagcc tctccctgtc tccgggtaaa tctagagggc ccttcgaagg
     3241 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
     3301 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctaagg cctgagctcg
     3361 ctgatcagcc tcgatcgagg atccagacat gataagatac attgatgagt ttggacaaac
     3421 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     3481 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     3541 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     3601 tggtatggct gattatgatc agtcgacctg caggcatgca agcttggcgt aatcatggtc
     3661 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3721 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3781 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3841 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4621 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4741 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4801 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4861 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4921 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4981 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5041 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5101 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5161 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5221 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5281 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5341 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5401 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5461 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5521 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5581 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5641 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5701 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5761 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     5821 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     5881 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     5941 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     6001 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     6061 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     6121 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     6181 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     6241 ttcccagtca cgacgtt
//
