LOCUS       sequence-298788-        9700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An all-in-one bicistronic plasmid for controlling protein 
            degradation by AID technology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Yesbolatova A, Saito Y, Kanemaki MT
  TITLE     Constructing Auxin-Inducible Degron Mutants Using an All-in-One 
            Vector.
  JOURNAL   Pharmaceuticals (Basel). 2020 May 23;13(5). pii: ph13050103. doi: 
            10.3390/ph13050103.
  PUBMED    32456235
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pharmaceuticals (Basel)."; date: "2020-05-23"; volume: "13(5). pii:
            ph13050103. doi"; pages: " 10.3390/ph13050103"
COMMENT     Sequence Label: pAID5.3-N
FEATURES             Location/Qualifiers
     source          1..9700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(678..689,690..852)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(883..916)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1479..2498)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(1479..2498)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     promoter        complement(2559..3058)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2607..2626)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3000..3023
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(3119..3152)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3183..3304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3194..3213)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3248..3267
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3485..3688)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3695..3751)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3767..5491)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(5536..5555)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5563..6579
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5617..5639)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6580..6857)
                     /label=chicken beta-actin promoter
     enhancer        6859..7238
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    join(7260..7447,7448..7459)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7516..7536)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7516..7534)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7555..7571)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7555..7571)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7568..7590)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7579..7595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7603..7609,7610..7627,7628..7633))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7648..7669
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7786..7803)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7957..8545)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8037..8056)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8716..9507,9508..9576))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9339..9358
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9577..9681)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc ggcgggtgtg gyggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
      661 cggcatatgg ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      721 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      781 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      841 taaaagcaag aaagatctta gtgaacctct tcgagggacc taataacttc gtatagcata
      901 cattatacga agttatatta agggttccgg atctcgacca gcttctgatg gaattagaac
      961 ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctt
     1021 caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca
     1081 agagaaaaac aatgatccca cgagagatct atagatctat agatcatgag tgggaggaat
     1141 gagctggccc ttaatttggt tttgcttgtt taaattatga tatccaacta tgaaacatta
     1201 tcataaagca atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca
     1261 cccccacccc cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt
     1321 cttaacaaag tatgacagga aaaacttcca ttttagtgga catctttatt gtttaataga
     1381 tcatcaattt ctgcatcccg gggatctgat atcatcgatg catggggtcg tgcgctcctt
     1441 tcggtcgggc gctgcgggtc gtggggcggg cggctagcct attcctttgc cctcggacga
     1501 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
     1561 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
     1621 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
     1681 tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc
     1741 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
     1801 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
     1861 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
     1921 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
     1981 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
     2041 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
     2101 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg
     2161 accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
     2221 gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc
     2281 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
     2341 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
     2401 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
     2461 agaaacttct cgacagacgt cgcggtgagt tcaggctttc cggatctatc catgctagcg
     2521 gtaagcttca gctgctcgag atctagatgg atgcaggtcg aaaggcccgg agatgaggaa
     2581 gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc agaatgccgg gcctccggag
     2641 gaccttcggg cgcccgcccc gcccctgagc ccgcccctga gcccgccccc ggacccaccc
     2701 cttcccagcc tctgagccca gaaagcgaag gagcaaagct gctattggcc gctgccccaa
     2761 aggcctaccc gcttccattg ctcagcggtg ctgtccatct gcacgagact agtgagacgt
     2821 gctacttcca tttgtcacgt cctgcacgac gcgagctgcg gggcgggggg gaacttcctg
     2881 actaggggag gagtagaagg tggcgcgaag gggccaccaa agaacggagt cggttggcgc
     2941 ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc
     3001 agcggggctg ctaaagcgca tgctccagac tgccttggga aaagcgcctc ccctacccgg
     3061 tagaatttcg aggtcgagat cctaagcttg gctggacgta aactcctctt cagacctaat
     3121 aacttcgtat agcatacatt atacgaagtt atattaaggg ttattgaata tgatcgacgc
     3181 gttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3241 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3301 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     3361 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
     3421 atccggtgga tcccgggccc gcggtaccgt cgactgcaga attcgaagct tgggcgcctg
     3481 cacctttata catcctcaaa tcgattttcc tcaagtacgg tgctccgtcc attgatacct
     3541 tcacgaacgc cgccgcctcc gggccaccgc ttgatttttg gcaggaaacc atcacgttct
     3601 tccggtatga tctcaccggt ggccatccca caacttgtgc cttggccgga ggtttggctg
     3661 gatctttagg acaagcactc ttctccttag atctaggtcc agggttctcc tccacgtctc
     3721 cagcctgctt cagcaggctg aagttagtag ctccgcttcc tgatctcaga atcttcacaa
     3781 agttgggagc atcatctctt gcccctgcgg tggtccgata cacatacagt ttctccacct
     3841 ttggcaggtc gccatgattt tcctccattt cgttagaccc gtcccgctca ttaatgactt
     3901 ccacgttcag cataggcatt ttactagcca ggacctgaca gcccttcaga gtcacattgc
     3961 aactagacat ccacaggctc ctcattgtct cgtatctggc aaagtttccc agcagagcgg
     4021 catccccgaa agggctgtcc cggatttcca gttttcgcag attcttacac ccgttcatca
     4081 cgtgcatcat gcccttatcg gagtctccgg caaatgcaat gctcagcatc tccagctgct
     4141 tagcatactt gccgatgtac atgaacactt tgtctgtcag caggccggaa atgctcagcc
     4201 tctggagtcc cttgcactct ctcacgatag ctccaaaccc ttcatccagt ggctgggatg
     4261 tgaccacgtc gggtttgcct ggttccagaa tacacagccg aaatcgggtg aaattagggc
     4321 agttcttggc gacggtcacc agtgcagcat tagtcatctg atgacagaaa tacagcaggc
     4381 tgttcagctt tgggcatccc agtgagactg ccaccaggcc ttcctcagtg acagcactat
     4441 atccggccac gtaaaaatca gatgggaaca ctctcagctc ctggaggtcc ttgcaggagc
     4501 tggcgaccac ctggagcccc ttatcggaaa tacagtccag cacccacagt cgctggagtt
     4561 tcacgcagcg tgagatcatc tttgtcagat cactggcgtc cagggttggt gcatagctca
     4621 ggttcaggcc tgtcagctgt gcacacagag ggtagataaa gctcaggcac acgggtgaag
     4681 catcccagaa tcctgacaga ctcctcagca ttttacactt ctccagggca gaagtcagtt
     4741 taaagtagga ttctgtctgg aaatcgtctg tcagattgcc ggttcccaga tcctccaggt
     4801 taggggttct cagcagaatc ttagccagag tgtccacgct cacgctccgg ttcagccgca
     4861 gagacctcag gttggggctt cttgagacca gccgctccag ggacccagca ttcacttcgc
     4921 ctttgatgca ggcaaagttc agggacacca ggctggtaca tgagtctggg aagcaagaca
     4981 gccatctggg gcctctatcc tccacttcat tctcctggag gtccagctcc cgcagcagct
     5041 tacagtggga ggccacggct gccagtccat cggtgctgaa gccttcgcaa gaaatcagca
     5101 ccagtgcgcg aaacctaggg aagctccgag ccagcagctc cagactttcg tcagagacca
     5161 ccattcgctt catgcgcagt tcctccagtc catgacagcc gcgagcggct gcctcgatcc
     5221 aagggccggc gtatcctccc caatcagggg gcaccaggtt aaagtcggcg aagtggggtt
     5281 tccccttgac ggtcagtgcg cgcacatttg gaaacctagc ggcgaccctg cctgctctca
     5341 cagcatagca gttgccgacg aacactcctc tccggctcag gcgttcgatc tcgtaccaca
     5401 ctttgcagac caggctcact gtgtttctat ctctctgtgc aggcaggaag ctaaaaatgt
     5461 gttcgacgac ctcttcagga aagtatgtca tggtggcagc gcttcgagga attctttgcc
     5521 aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga aaaagaagaa
     5581 ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga agccgaaccc
     5641 cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc gaggctggag
     5701 agggagaagg ggacggcggc gcggcgacgc acgaaggccc tccccgccca tttccttcct
     5761 gccggcgccg caccgcttcg ccccgcgccc gctagagggg gtgcggcggc gcctcccaga
     5821 tttcggctcc gcacagattt gggacaaagg aagtccctgc gccctctcgc acgattacca
     5881 taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg ccggcgctcc gggggccgcc
     5941 gcgcccctcc cccgagcccy ccccggcccg aggcggcccc gccccgcccg gcacccccac
     6001 ctgccgccac cccccgcccg gcacggcgag ccccgcgcca cgccccgtac ggagccccgc
     6061 acccgaagcc gggccgtgct cagcaactcg gggagggggg tgcagggggg gttgcagccc
     6121 gaccgacgcg cccacacccc ctgctcaccc ccccacgcac acaccccgca cgcagccttt
     6181 gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc ccggccgcgc tcccctcgcg
     6241 cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag
     6301 cgcggagccg cacgcggcgc tccccacgca cacacacacg cacgcacccc ccgagccgct
     6361 ccccccgcac aaagggccct cccggagccc ctcaaggctt tcacgcagcc acagaaaaga
     6421 aacaagccgt cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc
     6481 gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcggc
     6541 ggagcggggc acggggcgaa ggcagcgcgc agcgactccc gcccgccgcg cgcttcgctt
     6601 tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga gcgcgccgct
     6661 ctgattggct gccgccgcac ctctccgcct csccccsccc csccccycsc cccsccccsc
     6721 cccsccyggc gcgcgccccc cccccccccc cgcccccatc gctgcacaaa ataattaaaa
     6781 aataaataaa tacaaaattg ggggtgggga ggggggggag atggggagag tgaagcagaa
     6841 cgtggggctc acctcgacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     6901 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     6961 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     7021 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     7081 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     7141 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     7201 aattgattac tattaataac tagtcaataa tcaatgtcga gcatgcatct agagggccca
     7261 agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg agtaaaaagt
     7321 actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt ttaattgtac
     7381 tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag taaaattact
     7441 caagtacttt acacctctgg ttcttgaccc cctaccttca gcaagcccag cagatccgag
     7501 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     7561 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7621 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7681 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7741 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7801 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7861 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7921 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7981 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8041 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8101 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8161 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8221 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8281 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8341 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8401 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8461 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8521 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8581 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8641 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8701 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8761 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8821 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8881 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8941 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9001 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9061 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9121 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9181 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9241 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9301 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9361 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9421 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9481 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9541 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9601 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9661 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
//
