LOCUS       sequence-298795-        6827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bait vector GSO1 L3_pECIA2 should be used with prey vector GSO1 
            L3_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: GSO1 L3_pECIA2
FEATURES             Location/Qualifiers
     source          1..6827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             3080..3103
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3809..3850
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3860..3877
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3900..3917)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3950..4084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3974..3993)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4028..4047
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4228..4244)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4228..4244)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4241..4263)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4252..4268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4276..4282,4283..4300,4301..4306))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4321..4342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4459..4476)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4630..5218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4710..4729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5389..6180,6181..6249))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6012..6031
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6250..6354)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6422..6440
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6478..6500)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6600..6619
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(6813..6827,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg caaccgggta tcatcaacaa
      541 cgatcttcag actcttctcg aggtgaagaa atctttggtc acgaatccac aagaagacga
      601 tcctctccga caatggaact ctgataacat caactactgt tcttggaccg gagtcacatg
      661 cgacaatacc ggtttatttc gtgtaattgc tctaaacctt accggtttag gattaaccgg
      721 ttcaatttca ccttggtttg gccggtttga caacctaatc caccttgatc tatcttccaa
      781 caatctagtt ggtcccatcc caactgctct ctccaacctt acttccttgg agtctctgtt
      841 tctcttttct aaccaactca ccggcgagat tccgagccag ctcggctccc tcgttaatat
      901 ccggtcgcta aggatcggag acaatgagct tgttggagat atcccggaga cgttagggaa
      961 cttggtgaat ctccagatgc ttgctctggc ttcttgtaga ctcaccggtc ctataccgag
     1021 tcagctcggt cgtctcgtcc gagttcagtc gttgatattg caagataact atctcgaagg
     1081 accgattccg gctgagttag gaaactgctc tgatctcact gtgttcactg cggcggagaa
     1141 catgctcaac gggacgatac cggcggagct tggacgcctt gaaaacctcg aaatactcaa
     1201 cctggcgaac aacagtctca ccggagagat accgagtcaa ttaggtgaaa tgagccaact
     1261 ccagtatctt agcttgatgg caaatcaact tcaaggtctt atccccaaga gtttggctga
     1321 tctgggaaat cttcaaactc ttgatttatc ggcgaataat cttaccggag agatacctga
     1381 agaattctgg aacatgagtc agcttcttga tttggttcta gcaaacaatc atctctctgg
     1441 ttcattacca aagagcattt gttctaacaa cacaaacttg gagcaactgg ttttgtctgg
     1501 aactcagctt tctggtgaaa tccctgtgga attaagcaaa tgtcaatcgc ttaagcagct
     1561 tgatttgtcg aacaattctc tcgctggctc gatccctgaa gcattgtttg agctagtcga
     1621 actcacagat ctttatctcc acaataatac cttggagggt acgttatctc cttcgatatc
     1681 aaacctcact aatctacagt ggcttgtgtt gtatcacaat aacttggaag gcaagttacc
     1741 gaaggagatc agcgcactca gaaaattaga ggttctgttt ctgtatgaga atcgtttctc
     1801 aggggaaatc ccacaggaga ttggaaactg cacaagcttg aagatgattg atatgttcgg
     1861 gaatcatttc gaaggagaga tacctccttc aataggaaga ttaaaagagc ttaacttgct
     1921 tcacttgaga cagaacgagc ttgtgggtgg acttcccgcc agtttaggta actgtcatca
     1981 actgaacatc ttggatttgg cggataatca gctctcggga tcaattcctt catccttcgg
     2041 gttcttaaag ggcttggaac agttgatgct ttacaacaat tctcttcaag gaaatcttcc
     2101 tgattcactt atcagtctga ggaacctcac cagaatcaat ctctctcaca acaggctcaa
     2161 tggtacgatt catccattat gtggttcgag ctcatatctc tcattcgatg tcaccaacaa
     2221 cgggtttgaa gatgaaatcc ccctggagct aggaaactct caaaatcttg atcgtctcag
     2281 gttaggcaag aatcaattga caggaaaaat cccttggacg ttggggaaaa tccgcgagct
     2341 gtccttgtta gatatgtcaa gcaacgctct cacaggaacc attccgctac agctcgtctt
     2401 gtgcaagaaa ctgacacaca tcgatctcaa taacaatttt ctatcaggtc ctatacctcc
     2461 atggcttgga aaactttcac agttaggaga gctcaagctt tcttcgaatc agtttgttga
     2521 gtctttacct actgaactgt tcaactgtac aaagctgctt gtgttgtctc ttgatggaaa
     2581 ttcgcttaat gggtcgattc cacaagagat tggtaacttg ggagctctca atgtccttaa
     2641 ccttgataag aaccagtttt caggttcact tcctcaagct atgggaaagc tcagcaagct
     2701 gtatgagctt cggttatcaa gaaacagctt aacaggagaa atcccagttg agattggaca
     2761 gcttcaggat cttcaaagtg ctttagacct cagctacaat aacttcactg gagatatacc
     2821 ttcgacaatt gggacattat caaagctgga aacacttgat ctttctcaca atcagcttac
     2881 aggggaagtt cctggctcgg ttggggatat gaagagtttg ggatatctca atgtttcttt
     2941 caataacctc ggagggaagc tcaagaaaca gttctcaaga tggccagctg attccttttt
     3001 aggcaacaca ggtctttgtg gaagcccact aagtcgctgc aacagagtta gaagtaataa
     3061 caagcaacaa ggtcttagtc tggaggtgct gttccaggga ccagggagcg acaaaactca
     3121 cacatgccca ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
     3181 cccaaaaccc aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt
     3241 ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
     3301 gcataatgcc aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag
     3361 cgtcctcacc gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc
     3421 caacaaagcc ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg
     3481 agaaccacag gtgtacaccc tgcccccatc ccgggaggag atgaccaaga accaggtcag
     3541 cctgacctgc ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa
     3601 tgggcagccg gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
     3661 cttcctctac agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc
     3721 atgctccgtg atgcatgagg gtctgcacaa ccactacacg cagaagagcc tctccctgtc
     3781 tccgggtaaa tctagagggc ccttcgaagg taagcctatc cctaaccctc tcctcggtct
     3841 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     3901 ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg atccagacat
     3961 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     4021 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4081 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     4141 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agtcgacctg
     4201 caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     4261 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4321 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4381 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4441 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4501 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4561 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4621 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4681 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4741 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4801 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4861 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4921 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4981 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5041 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5101 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5161 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5221 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5281 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5341 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5401 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5461 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5521 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5581 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5641 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5701 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5761 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5821 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5881 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5941 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6001 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6061 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6361 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6421 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     6481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6541 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     6601 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     6661 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     6721 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     6781 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgtt
//
