LOCUS       sequence-298826-        6167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bait vector BAM3 X078_pECIA2 should be used with prey vector BAM3 
            X078_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: BAM3 X078_pECIA2
FEATURES             Location/Qualifiers
     source          1..6167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             2420..2443
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3149..3190
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3200..3217
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3240..3257)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3290..3424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3314..3333)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3368..3387
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3568..3584)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3568..3584)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3581..3603)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3592..3608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3616..3622,3623..3640,3641..3646))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3661..3682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3799..3816)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3970..4558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4050..4069)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4729..5520,5521..5589))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5352..5371
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5590..5694)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5762..5780
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5818..5840)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5940..5959
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(6153..6167,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg tctttgatct cacctcttaa
      541 tctctcactt attagacaag caaatgtcct tatctctcta aagcaaagtt ttgattccta
      601 tgatccttct cttgattcat ggaacattcc aaatttcaac tctctatgtt cttggactgg
      661 tgtttcttgt gacaacttga atcagtctat tactcgtcta gacctatcta atctcaacat
      721 ctccggcact atctctccgg aaatatctcg tctttcgccg tcacttgttt ttcttgacat
      781 ttcttctaac agtttctccg gtgagcttcc taaagagatc tatgagctct caggcctcga
      841 agtgttaaac atctctagca atgtttttga aggagagctg gagacacgtg ggttcagtca
      901 aatgactcag cttgtgactc ttgacgctta cgacaacagc ttcaacggat cacttcctct
      961 gagtctaacc acactcactc gtctcgagca cttagatctt ggaggaaact acttcgacgg
     1021 tgagatccct agaagctatg gaagtttctt gagtctcaag tttctttctt tatctggtaa
     1081 tgatctccgt gggagaatcc ctaacgagct agcgaacatc acgactttgg tacagcttta
     1141 cttaggttac tacaacgatt accgcggtgg gatacctgca gatttcggga gattgatcaa
     1201 tcttgttcat ttggatttag ctaattgcag cttgaaagga tcaattcctg cagaattggg
     1261 gaatctcaag aacttggagg ttctgtttct tcagaccaat gagcttacag gctctgttcc
     1321 tcgagagtta gggaacatga caagcctcaa gactcttgat ctctccaaca actttcttga
     1381 aggagagatt cctctagagc tatctggact tcaaaagctt cagttgttta acctcttctt
     1441 caacagacta cacggcgaga tccctgagtt cgtatctgag cttcctgatc tgcaaatact
     1501 caagctttgg cacaacaatt tcaccggaaa gattccttcg aaactcggat caaacgggaa
     1561 cttgatcgag atcgatttgt ctaccaataa actcacaggt ttgatccctg agtcactctg
     1621 tttcggaaga agactaaaga ttctcattct cttcaacaac ttcttgttcg gtcctctccc
     1681 tgaagatctt ggccaatgtg aaccgctatg gagattccgt ctcggacaga actttctgac
     1741 aagtaagttg ccaaagggtt tgatttattt gccgaatctt tcgcttcttg agcttcaaaa
     1801 caactttttg actggagaaa tccccgaaga agaggcggga aatgcgcagt tttcgagcct
     1861 tactcagatc aatctgtcca acaacaggtt atccggaccg attcctggtt caatcagaaa
     1921 cctcagaagc cttcagattc ttcttctcgg tgcaaaccgg ttatcgggac agatccctgg
     1981 cgaaatcgga agtttgaaga gtcttctcaa gattgacatg agcagaaaca acttctcagg
     2041 caagtttcct cctgagtttg gtgattgcat gtcactcaca tatttagatt tgagtcacaa
     2101 ccagatttcc ggtcagattc cggttcagat atcgcagatt cggattctaa actatctgaa
     2161 tgtttcttgg aattccttta accaaagcct tcccaacgaa ctcggataca tgaagagttt
     2221 aacatcagca gatttctcac acaacaactt ctccggttca gtaccaactt cagggcaatt
     2281 ctcttacttc aacaacacgt cattccttgg aaaccctttt ctctgtggat tttcttcaaa
     2341 cccttgcaac ggttcccaaa accaatctca atctcagcta cttaaccaga acaacgcaag
     2401 atcccgaggt gaaatctccc tggaggtgct gttccaggga ccagggagcg acaaaactca
     2461 cacatgccca ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
     2521 cccaaaaccc aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt
     2581 ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
     2641 gcataatgcc aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag
     2701 cgtcctcacc gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc
     2761 caacaaagcc ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg
     2821 agaaccacag gtgtacaccc tgcccccatc ccgggaggag atgaccaaga accaggtcag
     2881 cctgacctgc ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa
     2941 tgggcagccg gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
     3001 cttcctctac agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc
     3061 atgctccgtg atgcatgagg gtctgcacaa ccactacacg cagaagagcc tctccctgtc
     3121 tccgggtaaa tctagagggc ccttcgaagg taagcctatc cctaaccctc tcctcggtct
     3181 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     3241 ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg atccagacat
     3301 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3361 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3421 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     3481 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agtcgacctg
     3541 caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3601 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     3661 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3721 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3781 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3841 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3901 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3961 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4021 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4081 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4141 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4201 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4261 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4321 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4381 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4441 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4501 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4561 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4621 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4681 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4741 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4801 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4861 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4921 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4981 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5041 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5101 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5161 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5221 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5281 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5341 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5401 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5461 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5521 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5581 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5641 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5701 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     5761 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     5821 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     5881 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     5941 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     6001 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     6061 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     6121 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgtt
//
