LOCUS       sequence-298831-       10424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An all-in-one bicistronic plasmid for controlling protein 
            degradation by AID technology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Yesbolatova A, Saito Y, Kanemaki MT
  TITLE     Constructing Auxin-Inducible Degron Mutants Using an All-in-One 
            Vector.
  JOURNAL   Pharmaceuticals (Basel). 2020 May 23;13(5). pii: ph13050103. doi: 
            10.3390/ph13050103.
  PUBMED    32456235
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pharmaceuticals (Basel)."; date: "2020-05-23"; volume: "13(5). pii:
            ph13050103. doi"; pages: " 10.3390/ph13050103"
COMMENT     Sequence Label: pAID5.4-N
FEATURES             Location/Qualifiers
     source          1..10424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(679..690,691..853)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(884..917)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1480..2499)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(1480..2499)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     promoter        complement(2560..3059)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2608..2627)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3001..3024
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(3120..3153)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3184..3305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3195..3214)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3249..3268
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(3486..4196,4197..4199,4200..4202))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3486..4196,4197..4199,4200..4202))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3528..3549)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3877..3896
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4136..4157
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(4209..4412)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(4419..4475)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4491..6215)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(6260..6279)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6287..7303
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6341..6363)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7304..7581)
                     /label=chicken beta-actin promoter
     enhancer        7583..7962
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    join(7984..8171,8172..8183)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8240..8260)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8240..8258)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8279..8295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8279..8295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8292..8314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8303..8319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8327..8333,8334..8351,8352..8357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8372..8393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8510..8527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8681..9269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8761..8780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9440..10231,10232..10300))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10063..10082
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10301..10405)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc nggcgggtgt ggtggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccggcatatg gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
      721 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
      781 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa
      841 gtaaaagcaa gaaagatctt agtgaacctc ttcgagggac ctaataactt cgtatagcat
      901 acattatacg aagttatatt aagggttccg gatctcgacc agcttctgat ggaattagaa
      961 cttggcaaaa caatactgag aatgaagtgt atgtggaaca gaggctgctg atctcgttct
     1021 tcaggctatg aaactgacac atttggaaac cacagtactt agaaccacaa agtgggaatc
     1081 aagagaaaaa caatgatccc acgagagatc tatagatcta tagatcatga gtgggaggaa
     1141 tgagctggcc cttaatttgg ttttgcttgt ttaaattatg atatccaact atgaaacatt
     1201 atcataaagc aatagtaaag agccttcagt aaagagcagg catttatcta atcccacccc
     1261 acccccaccc ccgtagctcc aatccttcca ttcaaaatgt aggtactctg ttctcaccct
     1321 tcttaacaaa gtatgacagg aaaaacttcc attttagtgg acatctttat tgtttaatag
     1381 atcatcaatt tctgcatccc ggggatctga tatcatcgat gcatggggtc gtgcgctcct
     1441 ttcggtcggg cgctgcgggt cgtggggcgg gcggctagcc tattcctttg ccctcggacg
     1501 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     1561 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     1621 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     1681 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
     1741 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     1801 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     1861 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     1921 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     1981 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     2041 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     2101 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
     2161 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     2221 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc
     2281 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     2341 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     2401 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     2461 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ccggatctat ccatgctagc
     2521 ggtaagcttc agctgctcga gatctagatg gatgcaggtc gaaaggcccg gagatgagga
     2581 agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga
     2641 ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc
     2701 ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca
     2761 aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg
     2821 tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct
     2881 gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag tcggttggcg
     2941 cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc
     3001 cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg
     3061 gtagaatttc gaggtcgaga tcctaagctt ggctggacgt aaactcctct tcagacctaa
     3121 taacttcgta tagcatacat tatacgaagt tatattaagg gttattgaat atgatcgacg
     3181 cgttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3241 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3301 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     3361 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta
     3421 gatccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcgaagc ttgggcgcct
     3481 gcacccttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
     3541 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
     3601 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
     3661 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
     3721 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
     3781 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
     3841 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
     3901 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
     3961 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
     4021 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
     4081 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
     4141 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc
     4201 atgctagctt tatacatcct caaatcgatt ttcctcaagt acggtgctcc gtccattgat
     4261 accttcacga acgccgccgc ctccgggcca ccgcttgatt tttggcagga aaccatcacg
     4321 ttcttccggt atgatctcac cggtggccat cccacaactt gtgccttggc cggaggtttg
     4381 gctggatctt taggacaagc actcttctcc ttagatctag gtccagggtt ctcctccacg
     4441 tctccagcct gcttcagcag gctgaagtta gtagctccgc ttcctgatct cagaatcttc
     4501 acaaagttgg gagcatcatc tcttgcccct gcggtggtcc gatacacata cagtttctcc
     4561 acctttggca ggtcgccatg attttcctcc atttcgttag acccgtcccg ctcattaatg
     4621 acttccacgt tcagcatagg cattttacta gccaggacct gacagccctt cagagtcaca
     4681 ttgcaactag acatccacag gctcctcatt gtctcgtatc tggcaaagtt tcccagcaga
     4741 gcggcatccc cgaaagggct gtcccggatt tccagttttc gcagattctt acacccgttc
     4801 atcacgtgca tcatgccctt atcggagtct ccggcaaatg caatgctcag catctccagc
     4861 tgcttagcat acttgccgat gtacatgaac actttgtctg tcagcaggcc ggaaatgctc
     4921 agcctctgga gtcccttgca ctctctcacg atagctccaa acccttcatc cagtggctgg
     4981 gatgtgacca cgtcgggttt gcctggttcc agaatacaca gccgaaatcg ggtgaaatta
     5041 gggcagttct tggcgacggt caccagtgca gcattagtca tctgatgaca gaaatacagc
     5101 aggctgttca gctttgggca tcccagtgag actgccacca ggccttcctc agtgacagca
     5161 ctatatccgg ccacgtaaaa atcagatggg aacactctca gctcctggag gtccttgcag
     5221 gagctggcga ccacctggag ccccttatcg gaaatacagt ccagcaccca cagtcgctgg
     5281 agtttcacgc agcgtgagat catctttgtc agatcactgg cgtccagggt tggtgcatag
     5341 ctcaggttca ggcctgtcag ctgtgcacac agagggtaga taaagctcag gcacacgggt
     5401 gaagcatccc agaatcctga cagactcctc agcattttac acttctccag ggcagaagtc
     5461 agtttaaagt aggattctgt ctggaaatcg tctgtcagat tgccggttcc cagatcctcc
     5521 aggttagggg ttctcagcag aatcttagcc agagtgtcca cgctcacgct ccggttcagc
     5581 cgcagagacc tcaggttggg gcttcttgag accagccgct ccagggaccc agcattcact
     5641 tcgcctttga tgcaggcaaa gttcagggac accaggctgg tacatgagtc tgggaagcaa
     5701 gacagccatc tggggcctct atcctccact tcattctcct ggaggtccag ctcccgcagc
     5761 agcttacagt gggaggccac ggctgccagt ccatcggtgc tgaagccttc gcaagaaatc
     5821 agcaccagtg cgcgaaacct agggaagctc cgagccagca gctccagact ttcgtcagag
     5881 accaccattc gcttcatgcg cagttcctcc agtccatgac agccgcgagc ggctgcctcg
     5941 atccaagggc cggcgtatcc tccccaatca gggggcacca ggttaaagtc ggcgaagtgg
     6001 ggtttcccct tgacggtcag tgcgcgcaca tttggaaacc tagcggcgac cctgcctgct
     6061 ctcacagcat agcagttgcc gacgaacact cctctccggc tcaggcgttc gatctcgtac
     6121 cacactttgc agaccaggct cactgtgttt ctatctctct gtgcaggcag gaagctaaaa
     6181 atgtgttcga cgacctcttc aggaaagtat gtcatggtgg cagcgcttcg aggaattctt
     6241 tgccaaaatg atgagacagc acaataacca gcacgttgcc caggagctgt aggaaaaaga
     6301 agaaggcatg aacatggtta gcagaggctc tagagccgcc ggtcacacgc cagaagccga
     6361 accccgccct gccccgtccc ccccgaaggc agccgtcccc ccgcggacag ccccgaggct
     6421 ggagagggag aaggggacgg cggcgcggcg acgcacgaag gccctccccg cccatttcct
     6481 tcctgccggc gccgcaccgc ttcgccccgc gcccgctaga gggggtgcgg cggcgcctcc
     6541 cagatttcgg ctccgcacag atttgggaca aaggaagtcc ctgcgccctc tcgcacgatt
     6601 accataaaag gcaatggctg cggctcgccg cgcctcgaca gccgccggcg ctccgggggc
     6661 cgccgcgccc ctcccccgag cccyccccgg cccgaggcgg ccccgccccg cccggcaccc
     6721 ccacctgccg ccaccccccg cccggcacgg cgagccccgc gccacgcccc gtacggagcc
     6781 ccgcacccga agccgggccg tgctcagcaa ctcggggagg ggggtgcagg gggggttgca
     6841 gcccgaccga cgcgcccaca ccccctgctc acccccccac gcacacaccc cgcacgcagc
     6901 ctttgttccc ctcgcagccc cccccgcacc gcggggcacc gcccccggcc gcgctcccct
     6961 cgcgcacact gcggagcgca caaagccccg cgccgcgccc gcagcgctca cagccgccgg
     7021 gcagcgcgga gccgcacgcg gcgctcccca cgcacacaca cacgcacgca ccccccgagc
     7081 cgctcccccc gcacaaaggg ccctcccgga gcccctcaag gctttcacgc agccacagaa
     7141 aagaaacaag ccgtcattaa accaagcgct aattacagcc cggaggagaa gggccgtccc
     7201 gcccgctcac ctgtgggagt aacgcggtca gtcagagccg gggcgggcgg cgcgaggcgg
     7261 cggcggagcg gggcacgggg cgaaggcagc gcgcagcgac tcccgcccgc cgcgcgcttc
     7321 gctttttata gggccgccgc cgccgccgcc tcgccataaa aggaaacttt cggagcgcgc
     7381 cgctctgatt ggctgccgcc gcacctctcc gccycscccc sccccscccc ccsccccscc
     7441 ccsccccscc yggcgcgcgc cccccccccc cccccgcccc catcgctgca caaaataatt
     7501 aaaaaataaa taaatacaaa attgggggtg gggagggggg ggagatgggg agagtgaagc
     7561 agaacgtggg gctcacctcg accatggtaa tagcgatgac taatacgtag atgtactgcc
     7621 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     7681 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     7741 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     7801 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     7861 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     7921 ccgtaattga ttactattaa taactagtca ataatcaatg tcgagcatgc atctagaggg
     7981 cccaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa
     8041 aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt
     8101 gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat
     8161 tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc
     8221 cgagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     8281 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8341 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8401 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8461 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8521 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8581 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8641 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8701 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8761 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8821 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8881 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8941 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9001 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9061 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9121 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9181 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10321 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtg
//
