LOCUS       sequence-298836-        9250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An all-in-one bicistronic plasmid for controlling protein 
            degradation by AID technology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Yesbolatova A, Saito Y, Kanemaki MT
  TITLE     Constructing Auxin-Inducible Degron Mutants Using an All-in-One 
            Vector.
  JOURNAL   Pharmaceuticals (Basel). 2020 May 23;13(5). pii: ph13050103. doi: 
            10.3390/ph13050103.
  PUBMED    32456235
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pharmaceuticals (Basel)."; date: "2020-05-23"; volume: "13(5). pii:
            ph13050103. doi"; pages: " 10.3390/ph13050103"
COMMENT     Sequence Label: pAID5.1-N
FEATURES             Location/Qualifiers
     source          1..9250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(679..690,691..853)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(884..917)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1474..2073)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2054..2073
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2113..2612)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2161..2180)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2554..2577
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(2673..2706)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2733..2854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2744..2763)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2798..2817
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3035..3238)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3245..3301)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3317..5041)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(5086..5105)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5113..6129
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5167..5189)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6130..6407)
                     /label=chicken beta-actin promoter
     enhancer        6409..6788
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    join(6810..6997,6998..7009)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7066..7086)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7066..7084)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7105..7121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7105..7121)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7118..7140)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7129..7145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7153..7159,7160..7177,7178..7183))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7198..7219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7336..7353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7507..8095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7587..7606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8266..9057,9058..9126))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8889..8908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9127..9231)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc nggcgggtgt ggtggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccggcatatg gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
      721 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
      781 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa
      841 gtaaaagcaa gaaagatctt agtgaacctc ttcgagggac ctaataactt cgtatagcat
      901 acattatacg aagttatatt aagggttccg gatctcgacc agcttctgat ggaattagaa
      961 cttggcaaaa caatactgag aatgaagtgt atgtggaaca gaggctgctg atctcgttct
     1021 tcaggctatg aaactgacac atttggaaac cacagtactt agaaccacaa agtgggaatc
     1081 aagagaaaaa caatgatccc acgagagatc tatagatcta tagatcatga gtgggaggaa
     1141 tgagctggcc cttaatttgg ttttgcttgt ttaaattatg atatccaact atgaaacatt
     1201 atcataaagc aatagtaaag agccttcagt aaagagcagg catttatcta atcccacccc
     1261 acccccaccc ccgtagctcc aatccttcca ttcaaaatgt aggtactctg ttctcaccct
     1321 tcttaacaaa gtatgacagg aaaaacttcc attttagtgg acatctttat tgtttaatag
     1381 atcatcaatt tctgcatccc ggggatctga tatcatcgat gcatggggtc gtgcgctcct
     1441 ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc gggtcatgca
     1501 ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc
     1561 gtagaagggg aggttgtggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc
     1621 ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg
     1681 cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag
     1741 gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc
     1801 gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc
     1861 ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag
     1921 ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc
     1981 ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt
     2041 ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttcagctgct cgagatctag
     2101 atggatgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg
     2161 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct
     2221 gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc
     2281 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc
     2341 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca
     2401 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc
     2461 gaaggggcca ccaaagaacg gagtcggttg gcgcctaccg gtggatgtgg aatgtgtgcg
     2521 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc
     2581 agactgcctt gggaaaagcg cctcccctac ccggtagaat ttcgaggtcg agatcctaag
     2641 cttggctgga cgtaaactcc tcttcagacc taataacttc gtatagcata cattatacga
     2701 agttatatta agggttattg aatatgatcg cttaagatac attgatgagt ttggacaaac
     2761 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2821 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2881 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     2941 tggtatggct gattatgatc agttatctag atccggtgga tcccgggccc gcggtaccgt
     3001 cgactgcaga attcgaagct tgggcgcctg cacctttata catcctcaaa tcgattttcc
     3061 tcaagtacgg tgctccgtcc attgatacct tcacgaacgc cgccgcctcc gggccaccgc
     3121 ttgatttttg gcaggaaacc atcacgttct tccggtatga tctcaccggt ggccatccca
     3181 caacttgtgc cttggccgga ggtttggctg gatctttagg acaagcactc ttctccttag
     3241 atctaggtcc agggttctcc tccacgtctc cagcctgctt cagcaggctg aagttagtag
     3301 ctccgcttcc tgatctcaga atcttcacaa agttgggagc atcatctctt gcccctgcgg
     3361 tggtccgata cacatacagt ttctccacct ttggcaggtc gccatgattt tcctccattt
     3421 cgttagaccc gtcccgctca ttaatgactt ccacgttcag cataggcatt ttactagcca
     3481 ggacctgaca gcccttcaga gtcacattgc aactagacat ccacaggctc ctcattgtct
     3541 cgtatctggc aaagtttccc agcagagcgg catccccgaa agggctgtcc cggatttcca
     3601 gttttcgcag attcttacac ccgttcatca cgtgcatcat gcccttatcg gagtctccgg
     3661 caaatgcaat gctcagcatc tccagctgct tagcatactt gccgatgtac atgaacactt
     3721 tgtctgtcag caggccggaa atgctcagcc tctggagtcc cttgcactct ctcacgatag
     3781 ctccaaaccc ttcatccagt ggctgggatg tgaccacgtc gggtttgcct ggttccagaa
     3841 tacacagccg aaatcgggtg aaattagggc agttcttggc gacggtcacc agtgcagcat
     3901 tagtcatctg atgacagaaa tacagcaggc tgttcagctt tgggcatccc agtgagactg
     3961 ccaccaggcc ttcctcagtg acagcactat atccggccac gtaaaaatca gatgggaaca
     4021 ctctcagctc ctggaggtcc ttgcaggagc tggcgaccac ctggagcccc ttatcggaaa
     4081 tacagtccag cacccacagt cgctggagtt tcacgcagcg tgagatcatc tttgtcagat
     4141 cactggcgtc cagggttggt gcatagctca ggttcaggcc tgtcagctgt gcacacagag
     4201 ggtagataaa gctcaggcac acgggtgaag catcccagaa tcctgacaga ctcctcagca
     4261 ttttacactt ctccagggca gaagtcagtt taaagtagga ttctgtctgg aaatcgtctg
     4321 tcagattgcc ggttcccaga tcctccaggt taggggttct cagcagaatc ttagccagag
     4381 tgtccacgct cacgctccgg ttcagccgca gagacctcag gttggggctt cttgagacca
     4441 gccgctccag ggacccagca ttcacttcgc ctttgatgca ggcaaagttc agggacacca
     4501 ggctggtaca tgagtctggg aagcaagaca gccatctggg gcctctatcc tccacttcat
     4561 tctcctggag gtccagctcc cgcagcagct tacagtggga ggccacggct gccagtccat
     4621 cggtgctgaa gccttcgcaa gaaatcagca ccagtgcgcg aaacctaggg aagctccgag
     4681 ccagcagctc cagactttcg tcagagacca ccattcgctt catgcgcagt tcctccagtc
     4741 catgacagcc gcgagcggct gcctcgatcc aagggccggc gtatcctccc caatcagggg
     4801 gcaccaggtt aaagtcggcg aagtggggtt tccccttgac ggtcagtgcg cgcacatttg
     4861 gaaacctagc ggcgaccctg cctgctctca cagcatagca gttgccgacg aacactcctc
     4921 tccggctcag gcgttcgatc tcgtaccaca ctttgcagac caggctcact gtgtttctat
     4981 ctctctgtgc aggcaggaag ctaaaaatgt gttcgacgac ctcttcagga aagtatgtca
     5041 tggtggcagc gcttcgagga attctttgcc aaaatgatga gacagcacaa taaccagcac
     5101 gttgcccagg agctgtagga aaaagaagaa ggcatgaaca tggttagcag aggctctaga
     5161 gccgccggtc acacgccaga agccgaaccc cgccctgccc cgtccccccc gaaggcagcc
     5221 gtccccccgc ggacagcccc gaggctggag agggagaagg ggacggcggc gcggcgacgc
     5281 acgaaggccc tccccgccca tttccttcct gccggcgccg caccgcttcg ccccgcgccc
     5341 gctagagggg gtgcggcggc gcctcccaga tttcggctcc gcacagattt gggacaaagg
     5401 aagtccctgc gccctctcgc acgattacca taaaaggcaa tggctgcggc tcgccgcgcc
     5461 tcgacagccg ccggcgctcc gggggccgcc gcgcccctcc cccgagcccy ccccggcccg
     5521 aggcggcccc gccccgcccg gcacccccac ctgccgccac cccccgcccg gcacggcgag
     5581 ccccgcgcca cgccccgtac ggagccccgc acccgaagcc gggccgtgct cagcaactcg
     5641 gggagggggg tgcagggggg gttgcagccc gaccgacgcg cccacacccc ctgctcaccc
     5701 ccccacgcac acaccccgca cgcagccttt gttcccctcg cagccccccc cgcaccgcgg
     5761 ggcaccgccc ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc
     5821 gcgcccgcag cgctcacagc cgccgggcag cgcggagccg cacgcggcgc tccccacgca
     5881 cacacacacg cacgcacccc ccgagccgct ccccccgcac aaagggccct cccggagccc
     5941 ctcaaggctt tcacgcagcc acagaaaaga aacaagccgt cattaaacca agcgctaatt
     6001 acagcccgga ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca
     6061 gagccggggc gggcggcgcg aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc
     6121 agcgactccc gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc
     6181 cataaaagga aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct
     6241 csccccsccc csccccycsc cccsccccsc cccsccyggc gcgcgccccc cccccccccc
     6301 cgcccccatc gctgcacaaa ataattaaaa aataaataaa tacaaaattg ggggtgggga
     6361 ggggggggag atggggagag tgaagcagaa cgtggggctc acctcgacca tggtaatagc
     6421 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     6481 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     6541 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     6601 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     6661 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     6721 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa
     6781 tcaatgtcga gcatgcatct agagggccca agtgatctcc aaaaaataag tactttttga
     6841 ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt
     6901 aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat
     6961 acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaccc
     7021 cctaccttca gcaagcccag cagatccgag ctccagcttt tgttcccttt agtgagggtt
     7081 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7141 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7201 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7261 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7321 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7381 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7441 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7501 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7561 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7621 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7681 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7741 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7801 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7861 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7921 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7981 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8041 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8101 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8161 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8221 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8281 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8341 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8401 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8461 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8521 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8581 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8641 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8701 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8761 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8821 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8881 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8941 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9001 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9061 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9121 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9181 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9241 ccgaaaagtg
//
