LOCUS       sequence-298837-       10427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An all-in-one bicistronic plasmid for controlling protein 
            degradation by AID technology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Yesbolatova A, Saito Y, Kanemaki MT
  TITLE     Constructing Auxin-Inducible Degron Mutants Using an All-in-One 
            Vector.
  JOURNAL   Pharmaceuticals (Basel). 2020 May 23;13(5). pii: ph13050103. doi: 
            10.3390/ph13050103.
  PUBMED    32456235
REFERENCE   2  (bases 1 to 10427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pharmaceuticals (Basel)."; date: "2020-05-23"; volume: "13(5). pii:
            ph13050103. doi"; pages: " 10.3390/ph13050103"
COMMENT     Sequence Label: pAID5.4-C
FEATURES             Location/Qualifiers
     source          1..10427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(679..690,691..853)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(884..917)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1480..2499)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(1480..2499)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     promoter        complement(2560..3059)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2608..2627)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3001..3024
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(3120..3153)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3184..3305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3195..3214)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3249..3268
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(3423..4136,4137..4139,4140..4142))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3426..4136,4137..4139,4140..4142))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3468..3489)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3817..3836
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4076..4097
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(4149..4352)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     misc_feature    4365..4421
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(4422..4478)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4494..6218)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(6263..6282)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6290..7306
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6344..6366)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7307..7584)
                     /label=chicken beta-actin promoter
     enhancer        7586..7965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    join(7987..8174,8175..8186)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8243..8263)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8243..8261)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8282..8298)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8282..8298)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8295..8317)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8306..8322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8330..8336,8337..8354,8355..8360))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8375..8396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8513..8530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8684..9272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8764..8783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9443..10234,10235..10303))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10066..10085
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10304..10408)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc nggcgggtgt ggtggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccggcatatg gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
      721 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
      781 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa
      841 gtaaaagcaa gaaagatctt agtgaacctc ttcgagggac ctaataactt cgtatagcat
      901 acattatacg aagttatatt aagggttccg gatctcgacc agcttctgat ggaattagaa
      961 cttggcaaaa caatactgag aatgaagtgt atgtggaaca gaggctgctg atctcgttct
     1021 tcaggctatg aaactgacac atttggaaac cacagtactt agaaccacaa agtgggaatc
     1081 aagagaaaaa caatgatccc acgagagatc tatagatcta tagatcatga gtgggaggaa
     1141 tgagctggcc cttaatttgg ttttgcttgt ttaaattatg atatccaact atgaaacatt
     1201 atcataaagc aatagtaaag agccttcagt aaagagcagg catttatcta atcccacccc
     1261 acccccaccc ccgtagctcc aatccttcca ttcaaaatgt aggtactctg ttctcaccct
     1321 tcttaacaaa gtatgacagg aaaaacttcc attttagtgg acatctttat tgtttaatag
     1381 atcatcaatt tctgcatccc ggggatctga tatcatcgat gcatggggtc gtgcgctcct
     1441 ttcggtcggg cgctgcgggt cgtggggcgg gcggctagcc tattcctttg ccctcggacg
     1501 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     1561 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     1621 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     1681 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
     1741 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     1801 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     1861 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     1921 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     1981 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     2041 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     2101 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
     2161 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     2221 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc
     2281 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     2341 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     2401 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     2461 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ccggatctat ccatgctagc
     2521 ggtaagcttc agctgctcga gatctagatg gatgcaggtc gaaaggcccg gagatgagga
     2581 agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga
     2641 ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc
     2701 ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca
     2761 aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg
     2821 tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct
     2881 gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag tcggttggcg
     2941 cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc
     3001 cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg
     3061 gtagaatttc gaggtcgaga tcctaagctt ggctggacgt aaactcctct tcagacctaa
     3121 taacttcgta tagcatacat tatacgaagt tatattaagg gttattgaat atgatcgacg
     3181 cgttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3241 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3301 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     3361 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta
     3421 gattacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
     3481 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
     3541 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
     3601 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
     3661 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
     3721 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
     3781 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
     3841 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
     3901 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
     3961 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
     4021 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
     4081 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc
     4141 atgctagctt tatacatcct caaatcgatt ttcctcaagt acggtgctcc gtccattgat
     4201 accttcacga acgccgccgc ctccgggcca ccgcttgatt tttggcagga aaccatcacg
     4261 ttcttccggt atgatctcac cggtggccat cccacaactt gtgccttggc cggaggtttg
     4321 gctggatctt taggacaagc actcttctcc ttggcgcctg caccggatcc cgggcccgcg
     4381 gtaccgtcga ctgcagaatt cgaagcttga gctcgagatc taggtccagg gttctcctcc
     4441 acgtctccag cctgcttcag caggctgaag ttagtagctc cgcttcctga tctcagaatc
     4501 ttcacaaagt tgggagcatc atctcttgcc cctgcggtgg tccgatacac atacagtttc
     4561 tccacctttg gcaggtcgcc atgattttcc tccatttcgt tagacccgtc ccgctcatta
     4621 atgacttcca cgttcagcat aggcatttta ctagccagga cctgacagcc cttcagagtc
     4681 acattgcaac tagacatcca caggctcctc attgtctcgt atctggcaaa gtttcccagc
     4741 agagcggcat ccccgaaagg gctgtcccgg atttccagtt ttcgcagatt cttacacccg
     4801 ttcatcacgt gcatcatgcc cttatcggag tctccggcaa atgcaatgct cagcatctcc
     4861 agctgcttag catacttgcc gatgtacatg aacactttgt ctgtcagcag gccggaaatg
     4921 ctcagcctct ggagtccctt gcactctctc acgatagctc caaacccttc atccagtggc
     4981 tgggatgtga ccacgtcggg tttgcctggt tccagaatac acagccgaaa tcgggtgaaa
     5041 ttagggcagt tcttggcgac ggtcaccagt gcagcattag tcatctgatg acagaaatac
     5101 agcaggctgt tcagctttgg gcatcccagt gagactgcca ccaggccttc ctcagtgaca
     5161 gcactatatc cggccacgta aaaatcagat gggaacactc tcagctcctg gaggtccttg
     5221 caggagctgg cgaccacctg gagcccctta tcggaaatac agtccagcac ccacagtcgc
     5281 tggagtttca cgcagcgtga gatcatcttt gtcagatcac tggcgtccag ggttggtgca
     5341 tagctcaggt tcaggcctgt cagctgtgca cacagagggt agataaagct caggcacacg
     5401 ggtgaagcat cccagaatcc tgacagactc ctcagcattt tacacttctc cagggcagaa
     5461 gtcagtttaa agtaggattc tgtctggaaa tcgtctgtca gattgccggt tcccagatcc
     5521 tccaggttag gggttctcag cagaatctta gccagagtgt ccacgctcac gctccggttc
     5581 agccgcagag acctcaggtt ggggcttctt gagaccagcc gctccaggga cccagcattc
     5641 acttcgcctt tgatgcaggc aaagttcagg gacaccaggc tggtacatga gtctgggaag
     5701 caagacagcc atctggggcc tctatcctcc acttcattct cctggaggtc cagctcccgc
     5761 agcagcttac agtgggaggc cacggctgcc agtccatcgg tgctgaagcc ttcgcaagaa
     5821 atcagcacca gtgcgcgaaa cctagggaag ctccgagcca gcagctccag actttcgtca
     5881 gagaccacca ttcgcttcat gcgcagttcc tccagtccat gacagccgcg agcggctgcc
     5941 tcgatccaag ggccggcgta tcctccccaa tcagggggca ccaggttaaa gtcggcgaag
     6001 tggggtttcc ccttgacggt cagtgcgcgc acatttggaa acctagcggc gaccctgcct
     6061 gctctcacag catagcagtt gccgacgaac actcctctcc ggctcaggcg ttcgatctcg
     6121 taccacactt tgcagaccag gctcactgtg tttctatctc tctgtgcagg caggaagcta
     6181 aaaatgtgtt cgacgacctc ttcaggaaag tatgtcatgg tggcagcgct tcgaggaatt
     6241 ctttgccaaa atgatgagac agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa
     6301 agaagaaggc atgaacatgg ttagcagagg ctctagagcc gccggtcaca cgccagaagc
     6361 cgaaccccgc cctgccccgt cccccccgaa ggcagccgtc cccccgcgga cagccccgag
     6421 gctggagagg gagaagggga cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt
     6481 ccttcctgcc ggcgccgcac cgcttcgccc cgcgcccgct agagggggtg cggcggcgcc
     6541 tcccagattt cggctccgca cagatttggg acaaaggaag tccctgcgcc ctctcgcacg
     6601 attaccataa aaggcaatgg ctgcggctcg ccgcgcctcg acagccgccg gcgctccggg
     6661 ggccgccgcg cccctccccc gagcccyccc cggcccgagg cggccccgcc ccgcccggca
     6721 cccccacctg ccgccacccc ccgcccggca cggcgagccc cgcgccacgc cccgtacgga
     6781 gccccgcacc cgaagccggg ccgtgctcag caactcgggg aggggggtgc agggggggtt
     6841 gcagcccgac cgacgcgccc acaccccctg ctcacccccc cacgcacaca ccccgcacgc
     6901 agcctttgtt cccctcgcag ccccccccgc accgcggggc accgcccccg gccgcgctcc
     6961 cctcgcgcac actgcggagc gcacaaagcc ccgcgccgcg cccgcagcgc tcacagccgc
     7021 cgggcagcgc ggagccgcac gcggcgctcc ccacgcacac acacacgcac gcaccccccg
     7081 agccgctccc cccgcacaaa gggccctccc ggagcccctc aaggctttca cgcagccaca
     7141 gaaaagaaac aagccgtcat taaaccaagc gctaattaca gcccggagga gaagggccgt
     7201 cccgcccgct cacctgtggg agtaacgcgg tcagtcagag ccggggcggg cggcgcgagg
     7261 cggcggcgga gcggggcacg gggcgaaggc agcgcgcagc gactcccgcc cgccgcgcgc
     7321 ttcgcttttt atagggccgc cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg
     7381 cgccgctctg attggctgcc gccgcacctc tccgccycsc cccsccccsc cccccscccc
     7441 sccccscccc sccyggcgcg cgcccccccc ccccccccgc ccccatcgct gcacaaaata
     7501 attaaaaaat aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga
     7561 agcagaacgt ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact
     7621 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     7681 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     7741 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     7801 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     7861 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     7921 accccgtaat tgattactat taataactag tcaataatca atgtcgagca tgcatctaga
     7981 gggcccaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt
     8041 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta
     8101 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa
     8161 aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag
     8221 atccgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     8281 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8341 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8401 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8461 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9121 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9361 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9421 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9481 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9541 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9601 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9661 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9721 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9781 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9841 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9901 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9961 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10021 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10081 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10141 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10201 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10261 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10321 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10381 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtg
//
