LOCUS       sequence-298839-        5966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bait vector XIP1/CEPR1 X056_pECIA2 should be used with prey vector 
            XIP1/CEPR1 X056_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: XIP1/CEPR1 X056_pECIA2
FEATURES             Location/Qualifiers
     source          1..5966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             1594..1605
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2219..2242
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2948..2989
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2999..3016
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3039..3056)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3089..3223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3113..3132)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3167..3186
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3367..3383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3367..3383)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3380..3402)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3391..3407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3415..3421,3422..3439,3440..3445))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3460..3481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3598..3615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3769..4357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3849..3868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4528..5319,5320..5388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5151..5170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5389..5493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5561..5579
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5617..5639)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5739..5758
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(5952..5966,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg agttcgaacc aacaacctca
      541 attcttcaaa cttatgaaga actctctctt cggcgacgct ttgtccactt ggaatgtcta
      601 tgacgttggt accaactact gtaacttcac cggcgtaaga tgtgacggtc aagggctagt
      661 caccgatctt gatctttccg gtttgtcact ctccgggatt ttccctgatg gggtttgctc
      721 ttacttccca aacctccgtg tacttcgtct ctctcacaac catctcaaca aaagtagcag
      781 cttcctcaac accatcccta attgttctct gcttcgagac ctaaacatga gctctgtata
      841 tctcaaagga actctccctg atttctcaca gatgaaatct ttaagagtca tcgacatgtc
      901 ttggaaccac ttcaccggta gcttccctct ctctatcttc aatctcactg acttagagta
      961 tctcaatttc aatgagaatc cagagcttga tctatggact ctaccggatt ctgtctctaa
     1021 gctgacaaag ctcacacaca tgctcttgat gacgtgtatg ctccatggta atatcccaag
     1081 atccattggg aacttgactt ctcttgttga tcttgaactt agcggaaact tcctctccgg
     1141 tgagattcca aaagaaatag ggaacctttc caacctaagg cagcttgagc tttactataa
     1201 ctatcactta accgggagta taccggagga aatcggtaat ctcaagaatc ttacggatat
     1261 cgatatctcg gtaagtaggt taaccggaag cataccggat tcaatctgca gtctaccaaa
     1321 cctccgagtg cttcagctct acaacaacag cttaacaggt gagattccta agtcacttgg
     1381 caattcaaaa accttgaaga tcttgtctct ctacgacaac tacttaaccg gtgaactccc
     1441 gccaaatctc ggttcttcct cgcctatgat cgctcttgat gtctccgaaa accgcctttc
     1501 tggtcctctt ccggctcacg tttgcaaatc cggtaagctt ttgtactttc ttgtactaca
     1561 aaaccggttc actggttcga tccctgagac ttatgggagc tgcaagaccc tcatacggtt
     1621 ccgtgttgcg agcaaccgcc ttgttggaac catacctcaa ggagttatgt ctctgcctca
     1681 tgtctccatc atcgacttgg cttacaactc cttgtctggt ccaataccta atgcgatcgg
     1741 aaacgcttgg aatttgtcag agctgtttat gcagagtaac agaatctccg gtgttatacc
     1801 tcatgagctc tctcactcca ccaatcttgt gaagcttgac ctcagcaaca atcaactgtc
     1861 tggtccaata ccttcagaag tcggaagact taggaaactg aacttacttg tactgcaagg
     1921 caaccacctc gattcttcta tacctgattc actttctaat ctcaaatctc tcaacgttct
     1981 tgatctgtcc agtaatctcc tcactggaag aatacctgaa aacctctccg agttgctccc
     2041 aacctcgatt aatttctcca gcaaccggct ctctggtcct atccccgtct cattgataag
     2101 aggaggtctt gtagaaagct tttcggataa ccctaatctc tgtattccac caactgctgg
     2161 ttcatcagat ttgaagtttc cgatgtgtca agagcctcac ggcaagaaga agttgagcct
     2221 ggaggtgctg ttccagggac cagggagcga caaaactcac acatgcccac cgtgcccagc
     2281 acctgaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct
     2341 catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc
     2401 tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc
     2461 gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca
     2521 ggactggctg aatggcaagg agtacaagtg caaggtctcc aacaaagccc tcccagcccc
     2581 catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccct
     2641 gcccccatcc cgggaggaga tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg
     2701 cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta
     2761 caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctaca gcaagctcac
     2821 cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggg
     2881 tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat ctagagggcc
     2941 cttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
     3001 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc
     3061 ctgagctcgc tgatcagcct cgatcgagga tccagacatg ataagataca ttgatgagtt
     3121 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3181 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     3241 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     3301 ctacaaatgt ggtatggctg attatgatca gtcgacctgc aggcatgcaa gcttggcgta
     3361 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3421 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     3481 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     3541 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     3601 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3661 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4201 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4501 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5401 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5461 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5521 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     5581 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     5641 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     5701 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     5761 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     5821 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     5881 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     5941 gccagggttt tcccagtcac gacgtt
//
