LOCUS       sequence-298851-        5415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pBz109_CsoS2_NTD
FEATURES             Location/Qualifiers
     source          1..5415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             32..54
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             67..84
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(926..944)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      940..987
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1353..1358,1359..1375,1376..1381))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1408..1426)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1494..1598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1599..1667,1668..2459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1817..1836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2630..3218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3119..3138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3372..3389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3404..3543
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3469..3488)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3629..3651
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3645..3836)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     5294..5313
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     join(5397..5415,1)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5397..5415
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc
       61 atgtcccacc atcatcatca tcatccttca cagtcaggaa tgaatcctgc cgacctgagc
      121 ggactctctg gcaaggaact ggcacgcgca cgccgcgctg cactatccaa gcaagggaaa
      181 gcagctgttt ctaataaaac ggctagcgta aaccgtagca ctaaacaggc ggcatcttcg
      241 atcaatacaa atcaggtgcg ctcttctgta aatgaagtgc ccactgatta ccaaatggcg
      301 gatcaattgt gctctacgat tgatcatgct gactttggta ccgaaagcaa tcgcgttaga
      361 gatctctgcc gtcaacgcag agaggcacta tcaactatcg gtaaaaaagc ggttaaaacc
      421 aacggcaagc cgtcgggtcg cgttcgacca cagcaatcag tggttcacaa cgacgcaatg
      481 atcgaaaatg ccggtgatac taaccaatca tcgtccactt cattaaataa tgaactttcc
      541 gaaatctgct ccatagcaga cgacatgccg gagcgttttg gttcacaagc caaaaccgtc
      601 cgtgatatct gccgtgcacg ccgtcaagcg ctctctgagc gtggaactcg cgccgtgccg
      661 ccaaagccgc aatctcaagg tggtccagga cgcaatggct atcaaattga tggataccta
      721 gataccgcac ttcatggccg cgatgccgcc aagcgccacc gtgaaatgct ctgtcaatac
      781 ggccgcggca cagcaccttc ctgcaagcca acaggccgtg tcaaaaattc tgtacagtcg
      841 ggcaacgcag cgccaaaaaa ggtttaaagc ggttaagatc cggctgctaa caaagcccga
      901 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      961 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag
     1021 gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag
     1081 gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg
     1141 catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac
     1201 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
     1261 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
     1321 gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca
     1381 aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     1441 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1501 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1561 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1621 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1681 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1741 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1801 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     1861 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1921 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1981 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2041 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2101 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     2161 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2221 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2281 ttattgctga taaatctgga gccggtgagc gtggctctcg cggtatcatt gcagcactgg
     2341 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2401 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2461 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2521 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2581 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2641 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2701 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2761 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2821 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2881 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2941 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3001 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3061 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3121 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3181 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3241 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3301 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3361 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     3421 tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc
     3481 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     3541 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3601 tccgcttaca gacaagctgt gaccgcctcc gggagctgca tgtgtcagag gttttcaccg
     3661 tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat
     3721 tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat
     3781 gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat
     3841 gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg
     3901 atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt
     3961 aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag
     4021 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
     4081 atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg
     4141 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
     4201 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     4261 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg
     4321 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
     4381 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
     4441 gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
     4501 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
     4561 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga
     4621 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
     4681 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
     4741 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
     4801 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     4861 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     4921 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     4981 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     5041 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
     5101 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
     5161 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
     5221 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     5281 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     5341 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat
     5401 acgactcact atagg
//
