LOCUS       sequence-298861-        6611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bait vector FLS2 FLS2_pECIA2 should be used with prey vector FLS2 
            FLS2_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: FLS2 FLS2_pECIA2
FEATURES             Location/Qualifiers
     source          1..6611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             2864..2887
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3593..3634
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3644..3661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3684..3701)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3734..3868
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3758..3777)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3812..3831
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4012..4028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4012..4028)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4025..4047)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4036..4052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4060..4066,4067..4084,4085..4090))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4105..4126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4243..4260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4414..5002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4494..4513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5173..5964,5965..6033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5796..5815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6034..6138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6206..6224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6262..6284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6384..6403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(6597..6611,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg aagcagtcct tcgagcccga
      541 gatcgaggct ctgaagtcgt tcaagaacgg tatctccaac gaccccctgg gtgtcctgtc
      601 cgactggacc atcatcggtt ccctgcgtca ctgcaactgg accggtatca cctgcgactc
      661 caccggtcac gtggtgtccg tgtccctgct ggagaagcag ctggagggcg tgctgtcccc
      721 cgctatcgct aacctgacct acctgcaggt gctggacctg acctccaact ccttcaccgg
      781 caagatcccc gctgagatcg gcaagctgac cgagctgaac cagctgatcc tgtacctgaa
      841 ctacttctcc ggttccatcc cctccggtat ctgggagctg aagaacatct tctacctgga
      901 cctgcgtaac aacctgctgt ccggcgacgt gcccgaggaa atctgcaaga cctcctccct
      961 ggtgctgatc ggtttcgact acaacaacct gaccggaaag atccctgagt gcctgggtga
     1021 cctggtgcac ctgcagatgt tcgtggctgc tggcaaccac ctgactggct ccatccccgt
     1081 gtccatcggc accctggcta acctcaccga cctggacctc tccggcaacc agctcactgg
     1141 aaagatcccc cgtgacttcg gcaacctgct gaacctgcag tctctggtcc tgaccgagaa
     1201 cctgctggag ggcgacatcc ctgccgagat cggaaactgc tcctctctgg tgcagctgga
     1261 gctgtacgac aaccagctga ccggcaagat cccagccgag ctgggcaacc tggtccagct
     1321 gcaggctctg cgtatctaca agaacaagct gacctcctcc atcccttcct ccctgttccg
     1381 tctgacccag ctgacccacc tgggactgtc cgagaaccac ctcgtgggtc ccatctctga
     1441 ggaaatcggt ttcctggagt ccctggaggt gctgaccctg cactctaaca acttcaccgg
     1501 cgagttccct cagtccatca ccaacctgag gaacctgacc gtgctgaccg tcggtttcaa
     1561 caacatctct ggcgagctgc ccgctgacct gggtctgctg accaacctgc gcaacctgtc
     1621 cgctcacgac aacctcctca ccggtcccat tccatcctcc atctccaact gcaccggcct
     1681 gaagctgctc gacctgtccc acaaccagat gactggagaa atcccccgtg gtttcggtcg
     1741 tatgaacctg acattcatct ccatcggtcg taaccacttc actggcgaga tccctgacga
     1801 catcttcaac tgctccaacc tggagactct gtccgtggct gacaacaacc tcactggcac
     1861 cctgaagccc ctgatcggaa agctgcagaa gctgcgtatc ctgcaggtgt cctacaactc
     1921 cctgaccggt cctatccctc gcgagatcgg caacctcaag gacctgaaca tcctgtacct
     1981 ccactccaac ggtttcaccg gtcgtattcc ccgcgagatg tccaacctga ccctgctcca
     2041 gggcctgcgc atgtactcta acgacctgga gggccccatc ccagaggaaa tgttcgacat
     2101 gaagctgctg tccgtcctgg acctcagcaa caacaagttc tccggccaga tccctgctct
     2161 gttctccaag ctggagtctc tcacctacct gtctctgcag ggcaacaagt tcaacggttc
     2221 tatccccgct tccctcaagt ccctgtctct gctgaacacc ttcgacatct ccgacaacct
     2281 gctgactggt actatccccg gcgagctgct ggcttccctg aagaacatgc agctgtacct
     2341 caacttctcc aacaacctgc tcaccggaac catccccaag gaactgggaa agctggaaat
     2401 ggtgcaggaa atcgacctgt ctaacaacct cttctccggt agcatccctc gttcattgca
     2461 agcctgtaag aacgtgttca ccctggactt ctcccagaac aacctgtctg gtcacatccc
     2521 cgacgaggtg ttccagggca tggacatgat catctccctg aacctgtccc gtaactcctt
     2581 cagcggagag atcccacagt ccttcggcaa catgactcac ctggtgtctc tggacctgtc
     2641 ctctaacaac ctgaccggcg agatccccga gtctctggct aacctgtcca ctctgaagca
     2701 cctgaagctg gcttccaaca acctcaaggg tcacgtgccc gagtccggcg tgttcaagaa
     2761 catcaacgcc tccgacctga tgggcaacac cgacctgtgc ggttccaaga agcctctgaa
     2821 gccctgcacc atcaagcaga agtcctccca cttctccaag cgcctggagg tgctgttcca
     2881 gggaccaggg agcgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg
     2941 gggaccgtca gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac
     3001 ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa
     3061 ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta
     3121 caacagcacg taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg
     3181 caaggagtac aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat
     3241 ctccaaagcc aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga
     3301 ggagatgacc aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga
     3361 catcgccgtg gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc
     3421 cgtgctggac tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag
     3481 gtggcagcag gggaacgtct tctcatgctc cgtgatgcat gagggtctgc acaaccacta
     3541 cacgcagaag agcctctccc tgtctccggg taaatctaga gggcccttcg aaggtaagcc
     3601 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     3661 ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc
     3721 agcctcgatc gaggatccag acatgataag atacattgat gagtttggac aaaccacaac
     3781 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3841 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3901 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     3961 ggctgattat gatcagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct
     4021 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     4081 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4141 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     4201 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4261 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4321 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4381 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4441 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5041 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5101 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     5161 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     5221 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5281 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5341 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5401 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5461 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5521 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5581 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5641 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5701 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5761 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5821 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5881 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5941 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     6001 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     6061 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     6121 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     6181 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     6241 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6301 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6361 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     6421 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
     6481 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     6541 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     6601 gtcacgacgt t
//
