LOCUS       sequence-298866-        7714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggybacTransposon-based tunable and temporal expression control of 
            SARS-CoV2 N and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7714)
  TITLE     Inducible COVID19 SARS-CoV2 Nucleocapsid Expressing Plasmid in 
            Mammalian Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: XLone-BSD SARS-CoV2 N P2A mCherry
FEATURES             Location/Qualifiers
     source          1..7714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1679..1735
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1739..1762
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1763..2470
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1905..1923)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2173..2192
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2676..2810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3086..3120
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3131..3148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3131..3147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3140..3162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3356..3375)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3475..3497
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3535..3553)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3621..3725
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3726..3794,3795..4586)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3944..3963)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4757..5345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5246..5265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5499..5516
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5633..5654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5669..5674,5675..5692,5693..5699)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5707..5723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5712..5734
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5731..5747
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5731..5747
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5773..5835
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6011..6092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6209..6607)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(6608..6664)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6674..7417)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7418..7441)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7441..7450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7470..7681)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccgccaccat gagcgataac ggcccccaaa accaacggaa
      421 tgcccctcgg ataacatttg gtggccctag tgattccaca ggaagtaacc aaaacggaga
      481 acggtctgga gcccgctcta aacagaggcg accacaggga ttgccaaata acacagcgtc
      541 atggtttaca gccctgactc aacatggaaa ggaggacttg aaatttccac gaggacaggg
      601 tgtaccaata aacacaaaca gtagtccaga cgatcagatc gggtattata gaagggccac
      661 gagacgcatc agaggtggag atggtaaaat gaaagatctc agtccgcgct ggtactttta
      721 ttatttggga accggcccag aagcgggcct cccatatgga gccaataagg acgggattat
      781 atgggtggct acagagggag ccctcaatac gccaaaagat cacatcggaa caagaaaccc
      841 tgctaataat gccgcaatcg tgctgcagtt gcctcagggg acgacgcttc ctaaaggctt
      901 ttacgcagaa ggatcacgcg gcggcagcca agcatcctca aggtctagtt ccagaagtcg
      961 aaacagctcc agaaactcca caccagggtc cagtaggggc acaagtccgg cgcggatggc
     1021 gggcaacggc ggagacgccg cactcgctct gttgctcctg gaccggctca accaacttga
     1081 atccaagatg agtggtaagg gacagcagca acaaggtcaa accgtaacca agaaaagcgc
     1141 tgcagaagct tctaaaaaac ctcgacaaaa acggaccgct acgaaggcat ataacgttac
     1201 tcaagccttc gggagacgag ggccggagca aacccagggg aattttggag atcaggagct
     1261 catccggcaa gggacagatt acaaacattg gcctcaaatc gctcagttcg cgccgtccgc
     1321 aagtgctttt ttcggaatgt ctcgcatcgg tatggaagta acaccatccg ggacatggct
     1381 cacttatacc ggcgccatca agctggacga caaagatcca aatttcaaag atcaggtgat
     1441 tctcttgaat aaacacatag acgcgtataa gacgtttcca cccaccgaac ccaagaagga
     1501 caaaaagaaa aaagctgatg aaacacaagc gttgccccag cgacagaaga agcagcaaac
     1561 cgtcaccctc ctgccagccg ctgatctcga tgacttctcc aagcaacttc aacagtccat
     1621 gagctcagcc gattctactc aggcgctcga aggcggcggg ggagctagcg gcagcggcgc
     1681 cactaacttc tccctgttga aacaagcagg ggatgtcgaa gagaatcccg ggccaatgga
     1741 ctacaaggac gacgatgaca aggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     1801 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     1861 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1921 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     1981 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     2041 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     2101 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     2161 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     2221 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     2281 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     2341 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     2401 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     2461 gtacaagtaa gaattccgat catattcaat aacccactag tagaccacct cccctgcgag
     2521 ctaagctgga cagccaatga cgggtaagag agtgacattt ttcactaacc taagacagga
     2581 gggccgtcag agctactgcc taatccaaag acgggtaaaa gtgataaaaa tgtatcactc
     2641 caacctaaga caggcgcagc ttccgaggga tttgagatcc agacatgata agatacattg
     2701 atgagtttgg acaaaccaaa actagaatgc agtgaaaaaa atgccttatt tgtgaaattt
     2761 gtgatgctat tgccttattt gtaaccatta taagctgcaa taaacaagtt tgatatctat
     2821 aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa
     2881 ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc
     2941 acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg
     3001 gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga
     3061 aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg
     3121 ttaagaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     3181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     3241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     3301 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     3361 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     3421 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3481 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     3541 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     3601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3901 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3961 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4021 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4081 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4141 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4201 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4261 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4321 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4381 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4441 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4501 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4561 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4621 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4681 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4741 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4801 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4861 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4921 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4981 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5041 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5101 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5161 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5221 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5281 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5341 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5401 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5461 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5521 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5581 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5641 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5701 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     5761 aggtcgactt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     5821 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     5881 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     5941 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     6001 aaaactttta gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     6061 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     6121 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     6181 gtatggctga ttatgatcct ctggagattt agccctccca cacataacca gagggcagca
     6241 attcacgaat cccaactgcc gtcggctgtc catcactgtc cttcactatg gctttgatcc
     6301 caggatgcag atcgagaagc acctgtcggc accgtccgca ggggctcaag atgcccctgt
     6361 tctcatttcc gatcgcgacg atacaagtca ggttgccagc tgccgcagca gcagcagtgc
     6421 ccagcaccac gagttctgca caaggtcccc cagtaaaatg atatacattg acaccagtga
     6481 agatgcggcc gtcgctagag agagctgcgc tggcgacgct gtagtcttca gagatgggga
     6541 tgctgttgat tgtagccgtt gctctttcaa tgagggtgga ttcttcttga gacaaaggct
     6601 tggccatggg gccggggttc tcctccacgt cgccggcctg cttcagcagg ctgaagttgg
     6661 tggcgccgct gcccccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca
     6721 gcatatcaag gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt
     6781 caagggcgtc ggtcggcccg ccgctttcgc actttagctg tttctccagg ccacatatga
     6841 ttagttccag gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg
     6901 cttgtttcag aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta
     6961 cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag
     7021 cgtacagtgc gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca
     7081 gggtttcgta ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat
     7141 gtgagaggag agcacagcgg tatgacttgg cgttgttccg cagaaagtct tgccatgact
     7201 cgccttccag ggggcaggag tgggtatgat gcctgtccag catctcgatt ggcagggcat
     7261 cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct
     7321 tttgagcgag tttccttgtc gtcaggcctt cgataccgac tccattgagt aattccagag
     7381 cagagtttat gactttgctc ttgtccagtc tagacatctt atcgtcatcg tctttgtaat
     7441 ccatggtggc ggatcccgcg tcacgacacc tgtgttctgg cggcaaaccc gttgcgaaaa
     7501 agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg
     7561 cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg
     7621 gttcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc
     7681 cactgacggg caccggagcc actcgagtgg aatt
//
