LOCUS       sequence-298871-        5862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV2 TEV-NSP3
FEATURES             Location/Qualifiers
     source          1..5862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaaaacctgt acttccaagg catggcccct accaaggtga ccttcggaga cgacaccgtg
       61 atcgaggtgc agggttacaa gagcgtgaac atcaccttcg agctggacga acgcatcgac
      121 aaggtgctga acgaaaagtg ctccgcttac accgtggagc tgggcaccga ggtgaacgaa
      181 ttcgcctgcg tggtggccga cgctgtgatc aagaccctgc agccagtgtc cgaactgctg
      241 acccctctgg gtatcgacct ggacgagtgg tctatggcta cctactacct gttcgacgag
      301 agcggcgaat tcaagctggc ctctcacatg tactgcagct tctaccctcc cgacgaagac
      361 gaggaagagg gtgactgcga agaggaagag ttcgagcctt ccactcagta cgagtacggc
      421 accgaagacg actaccaggg caagcctctg gaattcggag ccacctctgc cgctctgcag
      481 cctgaagagg aacaggagga agactggctg gacgacgact cccagcagac cgtgggtcag
      541 caggacggct ctgaagacaa ccagaccacc accatccaga ccatcgtgga ggtgcagcct
      601 cagctggaga tggaactgac cccagtggtg cagaccatcg aggtgaacag cttctccggc
      661 tacctgaagc tgaccgacaa cgtgtacatc aagaacgctg acatcgtgga ggaagccaag
      721 aaggtgaaac caaccgtggt ggtgaacgcc gctaacgtgt acctgaagca cggcggaggt
      781 gtggctggag ctctgaacaa ggctaccaac aacgccatgc aggtggaatc cgacgactac
      841 atcgctacca acggtcctct gaaggtgggc ggatcctgcg tgctgtctgg ccacaacctg
      901 gccaagcact gcctgcacgt ggtgggacca aacgtgaaca agggagagga catccagctg
      961 ctgaagagcg cctacgaaaa cttcaaccag cacgaggtgc tgctggctcc actgctgtct
     1021 gccggaatct tcggtgctga tcccatccac agcctgcgcg tgtgcgtgga caccgtgagg
     1081 accaacgtgt acctggccgt gttcgacaag aacctgtacg acaagctggt gagctccttc
     1141 ctggagatga agtccgaaaa gcaggtggag cagaagatcg ccgagatccc caaggaggaa
     1201 gtgaagcctt tcatcaccga atctaagccc agcgtggagc agagaaagca ggacgacaag
     1261 aagatcaagg cctgcgtgga ggaagtgacc accaccctgg aggaaaccaa gttcctgacc
     1321 gagaacctgc tgctgtacat cgacatcaac ggcaacctgc accctgactc cgctaccctg
     1381 gtgtctgaca tcgacatcac cttcctgaag aaggacgccc cctacatcgt gggcgacgtg
     1441 gtgcaggaag gagtgctgac cgctgtggtc atccctacca agaaggccgg tggcaccacc
     1501 gagatgctgg ccaaggctct gcgtaaggtg ccaaccgaca actacatcac cacctacccc
     1561 ggtcagggcc tgaacggtta caccgtggag gaagctaaga ccgtgctgaa gaagtgcaag
     1621 tctgccttct acatcctgcc ctccatcatc tctaacgaga agcaggaaat cctgggcacc
     1681 gtgagctgga acctgcgcga aatgctggcc cacgctgagg aaaccaggaa gctgatgccc
     1741 gtgtgcgtgg agaccaaggc catcgtgagc accatccaga gaaagtacaa gggcatcaag
     1801 atccaggagg gtgtggtgga ctacggcgct cgtttctact tctacacctc caagaccacc
     1861 gtggcctctc tgatcaacac cctgaacgac ctgaacgaga ccctggtgac catgccactg
     1921 ggatacgtga cccacggtct gaacctggag gaagccgcta gatacatgcg ttccctgaag
     1981 gtgcctgcta ccgtgagcgt gagcagccct gacgctgtga ccgcctacaa cggatacctg
     2041 acctcctcta gcaagacccc tgaggaacac ttcatcgaaa ccatcagcct ggccggttcc
     2101 tacaaggact ggtcttacag cggacagtcc acccagctgg gcatcgagtt cctgaagcgc
     2161 ggagacaaga gcgtgtacta cacctccaac ccaaccacct tccacctgga cggtgaagtg
     2221 atcaccttcg acaacctgaa gaccctgctg tctctgcgcg aggtgaggac catcaaggtg
     2281 ttcaccaccg tggacaacat caacctgcac acccaggtgg tggacatgag catgacctac
     2341 ggacagcagt tcggtcctac ctacctggac ggagctgacg tgaccaagat caagccacac
     2401 aactcccacg agggcaagac cttctacgtg ctgcctaacg atgacaccct gagagtggag
     2461 gctttcgaat actaccacac caccgaccca tccttcctgg gcaggtacat gtctgccctg
     2521 aaccacacca agaagtggaa gtacccccag gtgaacggac tgaccagcat caagtgggcc
     2581 gacaacaact gctacctggc caccgctctg ctgaccctgc agcagatcga actgaagttc
     2641 aacccacccg ctctgcagga cgcttactac agagcccgtg ctggcgaggc cgctaacttc
     2701 tgcgctctga tcctggccta ctgcaacaag accgtgggcg aactgggaga cgtgagagag
     2761 accatgagct acctgttcca gcacgccaac ctggactcct gcaagcgtgt gctgaacgtg
     2821 gtgtgcaaga cctgcggcca gcagcagacc accctgaagg gagtggaagc tgtgatgtac
     2881 atgggtaccc tgtcctacga gcagttcaag aagggcgtgc agatcccttg cacctgcgga
     2941 aagcaggcca ccaagtacct ggtgcagcag gaaagcccat tcgtgatgat gtccgctcct
     3001 ccagcccagt acgaactgaa gcacggcacc ttcacctgcg cttccgagta caccggcaac
     3061 taccagtgcg gacactacaa gcacatcacc tctaaggaga ccctgtactg catcgacgga
     3121 gccctgctga ccaagtcctc tgaatacaag ggtcccatca ccgacgtgtt ctacaaggag
     3181 aacagctaca ccaccaccat caagcccgtg acctacaagc tggacggcgt ggtgtgcacc
     3241 gaaatcgacc ctaagctgga caactactac aagaaggaca actcttactt caccgagcag
     3301 cctatcgacc tggtgccaaa ccagccctac cctaacgcca gcttcgacaa cttcaagttc
     3361 gtgtgcgaca acatcaagtt cgctgacgac ctgaaccagc tgaccggata caagaagcct
     3421 gcctctagag agctgaaggt gaccttcttc ccagacctga acggcgacgt ggtggctatc
     3481 gactacaagc actacacccc aagcttcaag aagggagcca agctgctgca caagcccatc
     3541 gtgtggcacg tgaacaacgc taccaacaag gccacctaca agcctaacac ctggtgcatc
     3601 cgttgcctgt ggtctaccaa gccagtggaa acctccaact ctttcgacgt gctgaagagc
     3661 gaggacgctc agggcatgga caacctggcc tgcgaagacc tgaagcccgt gagcgaggaa
     3721 gtggtggaga acccaaccat ccagaaggac gtgctggaat gcaacgtgaa gaccaccgag
     3781 gtggtgggag acatcatcct gaagccagct aacaacagcc tgaagatcac cgaggaagtg
     3841 ggtcacaccg acctgatggc cgcttacgtg gacaacagct ccctgaccat caagaagccc
     3901 aacgagctga gcagggtgct gggtctgaag accctggcta ctcacggtct ggctgctgtg
     3961 aactctgtgc catgggacac catcgctaac tacgccaagc ctttcctgaa caaggtggtg
     4021 tccaccacca ccaacatcgt gaccagatgt ctgaaccgcg tgtgcaccaa ctacatgccc
     4081 tacttcttca ccctgctgct gcagctgtgc accttcacca gaagcaccaa ctcccgtatc
     4141 aaggctagca tgcctaccac catcgccaag aacaccgtga agagcgtggg aaagttctgc
     4201 ctggaggctt ccttcaacta cctgaagtct ccaaacttca gcaagctgat caacatcatc
     4261 atctggttcc tgctgctctc cgtgtgcctg ggttccctga tctactctac cgccgctctg
     4321 ggagtgctga tgtctaacct gggtatgcct agctactgca ctggttacag agagggatac
     4381 ctgaacagca ccaacgtgac catcgccacc tactgcaccg gcagcatccc atgctccgtg
     4441 tgcctgtctg gactggacag cctggacacc tacccctccc tggaaaccat ccagatcacc
     4501 atctctagct tcaagtggga cctgaccgcc ttcggtctgg tggctgagtg gttcctggcc
     4561 tacatcctgt tcaccaggtt cttctacgtg ctgggcctgg ccgctatcat gcagctgttc
     4621 ttctcctact tcgctgtgca cttcatcagc aactcctggc tgatgtggct gatcatcaac
     4681 ctggtgcaga tggctcccat ctctgccatg gtgcgcatgt acatcttctt cgccagcttc
     4741 tactacgtgt ggaagtccta cgtgcacgtg gtggacggtt gcaactcctc tacctgcatg
     4801 atgtgctaca agcgcaacag ggctaccagg gtggagtgca ccaccatcgt gaacggcgtg
     4861 cgcaggtcct tctacgtgta cgccaacggc ggcaagggat tctgcaagct gcacaactgg
     4921 aactgcgtga actgcgacac cttctgcgct ggctccacct tcatctctga cgaggtggcc
     4981 agagacctga gcctgcagtt caagcgtcca atcaacccca ccgaccagag ctcctacatc
     5041 gtggactctg tgaccgtgaa gaacggtagc atccacctgt acttcgacaa ggctggccag
     5101 aagacctacg agcgccactc tctgagccac ttcgtgaacc tggacaacct gagggccaac
     5161 aacaccaagg gcagcctgcc tatcaacgtg atcgtgttcg acggaaagtc caagtgcgag
     5221 gaatctagcg ccaagtccgc cagcgtgtac tacagccagc tgatgtgcca gccaatcctg
     5281 ctgctggacc aggctctggt gtctgacgtg ggagacagcg ccgaagtggc cgtgaagatg
     5341 ttcgacgcct acgtgaacac cttctcctct accttcaacg tgcctatgga gaagctgaag
     5401 accctggtgg ctactgctga ggctgaactg gctaagaacg tgtccctgga caacgtgctg
     5461 agcaccttca tctccgccgc taggcagggt ttcgtggact ctgacgtgga aaccaaagac
     5521 gtggtggagt gcctgaagct gtctcaccag agcgacatcg aagtgaccgg cgactcctgc
     5581 aacaactaca tgctgaccta caacaaggtg gagaacatga ccccaagaga cctgggagct
     5641 tgcatcgact gctccgcccg tcacatcaac gctcaggtgg ccaagtctca caacatcgcc
     5701 ctgatctgga acgtgaagga cttcatgagc ctgtccgagc agctgcgcaa gcagatcagg
     5761 agcgccgcta agaagaacaa cctgcccttc aagctgacct gcgctaccac cagacaggtg
     5821 gtgaacgtgg tgaccaccaa gatcgccctg aagggcggat aa
//
