LOCUS       sequence-298895-        7841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggybacTransposon-based tunable and temporal expression control of 
            SARS-CoV2 N and nuclear eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7841)
  TITLE     Inducible COVID19 SARS-CoV2 Nucleocapsid Expressing Plasmid in 
            Mammalian Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: XLone-Puro SARS-CoV2 N P2A eGFP-NLS
FEATURES             Location/Qualifiers
     source          1..7841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1679..1735
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1736..1738,1739..1741,1742..2452)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1736..1738,1739..1741,1742..2452)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1781..1802)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2042..2061)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2389..2410
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2453..2473
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2692..2826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3102..3136
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3147..3164)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3147..3163)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3156..3178)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3372..3391)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3491..3513
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3551..3569)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3637..3741
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3742..3810,3811..4602)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3960..3979)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4773..5361
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5262..5281
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5515..5532
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5649..5670
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5685..5690,5691..5708,5709..5715)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5723..5739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5728..5750
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5747..5763
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5747..5763
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5789..5851
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6027..6108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6129..6728)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6212..6232)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6709..6728
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(6738..6791)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6801..7544)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7545..7568)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7568..7577
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7597..7808)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccgccaccat gagcgataac ggcccccaaa accaacggaa
      421 tgcccctcgg ataacatttg gtggccctag tgattccaca ggaagtaacc aaaacggaga
      481 acggtctgga gcccgctcta aacagaggcg accacaggga ttgccaaata acacagcgtc
      541 atggtttaca gccctgactc aacatggaaa ggaggacttg aaatttccac gaggacaggg
      601 tgtaccaata aacacaaaca gtagtccaga cgatcagatc gggtattata gaagggccac
      661 gagacgcatc agaggtggag atggtaaaat gaaagatctc agtccgcgct ggtactttta
      721 ttatttggga accggcccag aagcgggcct cccatatgga gccaataagg acgggattat
      781 atgggtggct acagagggag ccctcaatac gccaaaagat cacatcggaa caagaaaccc
      841 tgctaataat gccgcaatcg tgctgcagtt gcctcagggg acgacgcttc ctaaaggctt
      901 ttacgcagaa ggatcacgcg gcggcagcca agcatcctca aggtctagtt ccagaagtcg
      961 aaacagctcc agaaactcca caccagggtc cagtaggggc acaagtccgg cgcggatggc
     1021 gggcaacggc ggagacgccg cactcgctct gttgctcctg gaccggctca accaacttga
     1081 atccaagatg agtggtaagg gacagcagca acaaggtcaa accgtaacca agaaaagcgc
     1141 tgcagaagct tctaaaaaac ctcgacaaaa acggaccgct acgaaggcat ataacgttac
     1201 tcaagccttc gggagacgag ggccggagca aacccagggg aattttggag atcaggagct
     1261 catccggcaa gggacagatt acaaacattg gcctcaaatc gctcagttcg cgccgtccgc
     1321 aagtgctttt ttcggaatgt ctcgcatcgg tatggaagta acaccatccg ggacatggct
     1381 cacttatacc ggcgccatca agctggacga caaagatcca aatttcaaag atcaggtgat
     1441 tctcttgaat aaacacatag acgcgtataa gacgtttcca cccaccgaac ccaagaagga
     1501 caaaaagaaa aaagctgatg aaacacaagc gttgccccag cgacagaaga agcagcaaac
     1561 cgtcaccctc ctgccagccg ctgatctcga tgacttctcc aagcaacttc aacagtccat
     1621 gagctcagcc gattctactc aggcgctcga aggcggcggg ggagctagcg gcagcggcgc
     1681 cactaacttc tccctgttga aacaagcagg ggatgtcgaa gagaatcccg ggccaatggt
     1741 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1801 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1861 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1921 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1981 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2041 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2101 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     2161 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     2221 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     2281 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2341 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2401 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agccaaagaa
     2461 gaagcgtaag gtataagtac aagtaagaat tccgatcata ttcaataacc cactagtaga
     2521 ccacctcccc tgcgagctaa gctggacagc caatgacggg taagagagtg acatttttca
     2581 ctaacctaag acaggagggc cgtcagagct actgcctaat ccaaagacgg gtaaaagtga
     2641 taaaaatgta tcactccaac ctaagacagg cgcagcttcc gagggatttg agatccagac
     2701 atgataagat acattgatga gtttggacaa accaaaacta gaatgcagtg aaaaaaatgc
     2761 cttatttgtg aaatttgtga tgctattgcc ttatttgtaa ccattataag ctgcaataaa
     2821 caagtttgat atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca
     2881 tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca
     2941 tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga
     3001 caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg
     3061 attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca
     3121 gactatcttt ctagggttaa gaattcactg gccgtcgttt tacaacgtcg tgactgggaa
     3181 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3241 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3301 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     3361 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     3421 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3481 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3541 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     3601 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3661 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3721 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3781 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3841 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3901 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3961 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4021 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4081 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4141 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4201 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4261 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4321 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4381 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4441 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4501 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4561 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4621 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4681 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4741 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4801 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4861 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4921 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4981 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5041 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5101 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5161 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5221 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5281 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5341 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5401 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5461 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5521 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     5581 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     5641 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     5701 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     5761 gaccatgatt acgccaaggt cgacttaacc ctagaaagat aatcatattg tgacgtacgt
     5821 taaagataat catgcgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta
     5881 tttattaatt tgaatagata ttaagtttta ttatatttac acttacatac taataataaa
     5941 ttcaacaaac aatttattta tgtttattta tttattaaaa aaaaacaaaa actcaaaatt
     6001 tcttctataa agtaacaaaa cttttagcag tgaaaaaaat gctttatttg tgaaatttgt
     6061 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa ctaaacgggc
     6121 cctctagatc aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc
     6181 tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg
     6241 gaggtctcca ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg
     6301 acggcgctgc ccagaccctt gccctggtgg tcgggcgaga cgccgacggt ggccaggaac
     6361 cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc
     6421 agccgggaac cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct
     6481 tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc
     6541 ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg
     6601 atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg
     6661 agggtgcgta cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac
     6721 tcggtcatct cgagcctagg gccgggattc tcctccacgt caccgcatgt tagaagactt
     6781 cctctgccct ctccgctgcc cccggggagc atgtcaaggt caaaatcgtc aagagcgtca
     6841 gcaggcagca tatcaaggtc aaagtcgtca agggcatcgg ctgggagcat gtctaagtca
     6901 aaatcgtcaa gggcgtcggt cggcccgccg ctttcgcact ttagctgttt ctccaggcca
     6961 catatgatta gttccaggcc gaaaaggaag gcaggttcgg ctccctgccg gtcgaacagc
     7021 tcaattgctt gtttcagaag tgggggcata gaatcggtgg taggtgtctc tctttcctct
     7081 tttgctactt gatgctcctg ttcctccaat acgcagccca gtgtaaagtg gcccacggcg
     7141 gacagagcgt acagtgcgtt ctccagggag aagccttgct gacacaggaa cgcgagctga
     7201 ttttccaggg tttcgtactg tttctctgtt gggcgggtgc cgagatgcac tttagccccg
     7261 tcgcgatgtg agaggagagc acagcggtat gacttggcgt tgttccgcag aaagtcttgc
     7321 catgactcgc cttccagggg gcaggagtgg gtatgatgcc tgtccagcat ctcgattggc
     7381 agggcatcga gcagggcccg cttgttcttc acgtgccagt acagggtagg ctgctcaact
     7441 cccagctttt gagcgagttt ccttgtcgtc aggccttcga taccgactcc attgagtaat
     7501 tccagagcag agtttatgac tttgctcttg tccagtctag acatcttatc gtcatcgtct
     7561 ttgtaatcca tggtggcgga tcccgcgtca cgacacctgt gttctggcgg caaacccgtt
     7621 gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg
     7681 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
     7741 ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
     7801 ctctgcccac tgacgggcac cggagccact cgagtggaat t
//
