LOCUS       sequence-298934-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses pBirA(1-G78)-FRB/biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Kwak C, Shin S, Park JS, Jung M, Nhung TTM, Kang MG, Lee C, Kwon TH,
            Park SK, Mun JY, Kim JS, Rhee HW
  TITLE     Contact-ID, a tool for profiling organelle contact sites, reveals 
            regulatory proteins of mitochondrial-associated membrane formation.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 
            10.1073/pnas.1916584117.
  PUBMED    32414919
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 
            10.1073/pnas.1916584117."
COMMENT     Sequence Label: pCDNA3/flag-pBirA(1-G78)-FRB
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             280..558
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 atggactaca aggatgacga cgataagatg aaggacaaca ccgtgcccct gaagctgatc
       61 gccctgctgg ccaacggcga gttccactct ggcgagcagc tgggagagac cctgggaatg
      121 agcagagccg ccatcaacaa gcacatccag acactgagag actggggagt ggacgtgttc
      181 accgtgcctg gcaagggcta cagcctgcct gagcctatcc agctgctgaa cgccaagcag
      241 atcctgggac agctggatgg cggatccatg gcttctagaa tcctctggca tgagatgtgg
      301 catgaaggcc tggaagaggc atctcgtttg tactttgggg aaaggaacgt gaaaggcatg
      361 tttgaggtgc tggagccctt gcatgctatg atggaacggg gcccccagac tctgaaggaa
      421 acatccttta atcaggccta tggtcgagat ttaatggagg cccaagagtg gtgcaggaag
      481 tacatgaaat cagggaatgt caaggacctc ctccaagcct gggacctcta ttatcatgtg
      541 ttccgacgaa tctcaaagac tagttaatga
//
