LOCUS       sequence-298938-        1185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses flag-pBirA(1-K283)-FRB/biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Kwak C, Shin S, Park JS, Jung M, Nhung TTM, Kang MG, Lee C, Kwon TH,
            Park SK, Mun JY, Kim JS, Rhee HW
  TITLE     Contact-ID, a tool for profiling organelle contact sites, reveals 
            regulatory proteins of mitochondrial-associated membrane formation.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 
            10.1073/pnas.1916584117.
  PUBMED    32414919
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 
            10.1073/pnas.1916584117."
COMMENT     Sequence Label: pCDNA3/flag-pBirA(1-K283)-FRB
FEATURES             Location/Qualifiers
     source          1..1185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             895..1173
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 atggactaca aggatgacga cgataagatg aaggacaaca ccgtgcccct gaagctgatc
       61 gccctgctgg ccaacggcga gttccactct ggcgagcagc tgggagagac cctgggaatg
      121 agcagagccg ccatcaacaa gcacatccag acactgagag actggggagt ggacgtgttc
      181 accgtgcctg gcaagggcta cagcctgcct gagcctatcc agctgctgaa cgccaagcag
      241 atcctgggac agctggatgg cggaagcgtg gccgtgctgc ctgtgatcga ctccaccaat
      301 cagtacctgc tggacagaat cggagagctg aagtccggcg acgcctgcat cgccgagtac
      361 cagcaggctg gcagaggagg cagaggacgg aagtggttca gcccattcgg agccaacctg
      421 tacctgtcca tgttctggag actggagcag ggacctgctg ctgccatcgg actgagtctg
      481 gtgatcggaa tcgtgatggc cgaggtgctg agaaagctgg gagccgacaa ggtgagagtg
      541 aagtggccta atgacctgta cctccaggac cgcaagctgg ctggcatcct ggtggagctg
      601 acaggcaaga caggcgatgc cgctcagatc gtgatcggag ccggaatcaa catggccatg
      661 agaagagtgg aggagagcgt ggtgaaccag ggctggatca ccctgcagga ggctggcatc
      721 aacctggacc ggaacaccct ggccgccatg ctgatcagag agctgagagc cgctctggag
      781 ctgttcgagc aggagggact ggctccttac ctgagcagat gggagaagct ggacaacttc
      841 atcaacagac ctgtgaagct gatcatcggc gacaagggat ccatggcttc tagaatcctc
      901 tggcatgaga tgtggcatga aggcctggaa gaggcatctc gtttgtactt tggggaaagg
      961 aacgtgaaag gcatgtttga ggtgctggag cccttgcatg ctatgatgga acggggcccc
     1021 cagactctga aggaaacatc ctttaatcag gcctatggtc gagatttaat ggaggcccaa
     1081 gagtggtgca ggaagtacat gaaatcaggg aatgtcaagg acctcctcca agcctgggac
     1141 ctctattatc atgtgttccg acgaatctca aagactagtt aatga
//
