LOCUS       sequence-298942-         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses flag-pbira(1-g78)-sec61b/biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Kwak C, Shin S, Park JS, Jung M, Nhung TTM, Kang MG, Lee C, Kwon TH,
            Park SK, Mun JY, Kim JS, Rhee HW
  TITLE     Contact-ID, a tool for profiling organelle contact sites, reveals 
            regulatory proteins of mitochondrial-associated membrane formation.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 
            10.1073/pnas.1916584117.
  PUBMED    32414919
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 
            10.1073/pnas.1916584117."
COMMENT     Sequence Label: pCDNA3/flag-pbira(1-g78)-sec61b
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             307..597
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
ORIGIN
        1 atggactaca aggatgacga cgataagaag gacaacaccg tgcccctgaa gctgatcgcc
       61 ctgctggcca acggcgagtt ccactctggc gagcagctgg gagagaccct gggaatgagc
      121 agagccgcca tcaacaagca catccagaca ctgagagact ggggagtgga cgtgttcacc
      181 gtgcctggca agggctacag cctgcctgag cctatccagc tgctgaacgc caagcagatc
      241 ctgggacagc tggatggcgg gggtgcgtca ggcggcagcg gatctggacc ggtcgccacc
      301 gaattcatgc ctggtccgac ccccagtggc actaacgtgg gatcctcagg gcgctctccc
      361 agcaaagcag tggccgcccg ggcggcggga tccactgtcc ggcagaggaa aaatgccagc
      421 tgtgggacaa ggagtgcagg ccgcacaacc tcggcaggca ccggggggat gtggcgattc
      481 tacacagaag attcacctgg gctcaaagtt ggccctgttc cagtattggt tatgagtctt
      541 ctgttcatcg cttctgtatt tatgttgcac atttggggca agtacactcg ttcgtga
//
