LOCUS       sequence-298950-        2100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses avitag-apex2-v5-fkbp8/biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Kwak C, Shin S, Park JS, Jung M, Nhung TTM, Kang MG, Lee C, Kwon TH,
            Park SK, Mun JY, Kim JS, Rhee HW
  TITLE     Contact-ID, a tool for profiling organelle contact sites, reveals 
            regulatory proteins of mitochondrial-associated membrane formation.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 
            10.1073/pnas.1916584117.
  PUBMED    32414919
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 
            10.1073/pnas.1916584117."
COMMENT     Sequence Label: pCDNA5/avitag-apex2-v5-fkbp8
FEATURES             Location/Qualifiers
     source          1..2100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..48
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             55..801
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             808..849
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgggcctga acgacatctt cgaggcccag aagatcgagt ggcacgaggc tagcggaaag
       61 tcttacccaa ctgtgagtgc tgattaccag gacgccgttg agaaggcgaa gaagaagctc
      121 agaggcttca tcgctgagaa gagatgcgct cctctaatgc tccgtttggc attccactct
      181 gctggaacct ttgacaaggg cacgaagacc ggtggaccct tcggaaccat caagcaccct
      241 gccgaactgg ctcacagcgc taacaacggt cttgacatcg ctgttaggct tttggagcca
      301 ctcaaggcgg agttccctat tttgagctac gccgatttct accagttggc tggcgttgtt
      361 gccgttgagg tcacgggtgg acctaaggtt ccattccacc ctggaagaga ggacaagcct
      421 gagccaccac cagagggtcg cttgcccgat cccactaagg gttctgacca tttgagagat
      481 gtgtttggca aagctatggg gcttactgac caagatatcg ttgctctatc tgggggtcac
      541 actattggag ctgcacacaa ggagcgttct ggatttgagg gtccctggac ctctaatcct
      601 cttattttcg acaactcata cttcacggag ttgttgagtg gtgagaagga aggtctcctt
      661 cagctacctt ctgacaaggc tcttttgtct gaccctgtat tccgccctct cgttgataaa
      721 tatgcagcgg acgaagatgc cttctttgct gattacgctg aggctcacca aaagctttcc
      781 gagcttgggt ttgctgatgc cggtaccggc aagcccatcc ccaaccccct gctgggcctg
      841 gacagcaccg cggccgcaat ggcatcgtgt gctgaaccct ctgagccctc tgccccactg
      901 cccgccgggg tcccaccgct cgaggacttc gaggtactgg atggggttga ggatgcagag
      961 ggtgaggagg aagaggagga ggaagaggag gaagaggatg acctgagtga gctgccaccg
     1021 ctggaggaca tgggacaacc cccggcggag gaggctgagc agcctggggc cctggcccga
     1081 gagttccttg ctgccatgga gcccgagccc gccccagccc cggccccaga agagtggctg
     1141 gacattctgg ggaacgggct gttgaggaag aagacgctgg tcccagggcc gccaggttcg
     1201 agccgcccgg tcaagggcca ggtggtcacc gtacatctgc agacgtcgct ggagaatggc
     1261 acacgggtgc aggaggagcc ggagctggtg ttcactctgg gtgactgtga cgtcatccag
     1321 gccctggatc tcagtgtccc actcatggac gtgggggaga cggccatggt cactgctgac
     1381 tccaagtact gctacggccc ccaaggcagc aggagcccat acatcccccc gcacgcggcc
     1441 ctgtgcctgg aggtgaccct gaagacggct gtggacgggc ctgacctgga gatgctcacg
     1501 gggcaggagc gcgtggccct ggccaaccgg aagcgggagt gcggcaacgc ccactaccag
     1561 cgggcggact tcgtcctggc cgccaactcc tacgacctcg ccatcaaggc tatcacctcc
     1621 agcgccaaag tggacatgac gttcgaggag gaggcacagc tcctgcagtt gaaggtgaag
     1681 tgtctgaaca acctggcggc ctcgcagctg aagctcgacc actaccgcgc agccctgcgc
     1741 tcctgcagcc ttgtgctgga gcaccagcca gacaacatca aggctctctt ccgcaagggc
     1801 aaggtgctgg cccagcaggg ggagtacagt gaggccatcc ccatcctgag ggcagccctg
     1861 aagctggaac cttccaacaa gacgatccac gcagagctct caaagctggt gaagaagcat
     1921 gcggcgcagc ggagcacgga gaccgccttg taccggaaaa tgctgggcaa ccccagccgg
     1981 ctgcctgcta agtgccctgg caagggtgcc tggtccatcc catggaagtg gctgtttggg
     2041 gcgactgctg ttgccttggg gggtgtggca ctctctgtgg tcatcgctgc caggaactag
//
