LOCUS       sequence-29897-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  TITLE     Howley Lab Plasmids
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHAGE-P-CMVt-N-HA Nedd4 wt
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..31
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    34..52
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             186..212
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    228..252
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttagcagagc tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac
       61 gagctcgctt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      121 catagaagac accgggaccg atccagcctc cggactctag cgtttaaacg ctagccacca
      181 tggcgtaccc atacgatgtt ccagattacg ctgctcgagg ttatcaaaca agtttgtaca
      241 aaaaagcagg ctccgcggcc gcccccttca ccatggcaac ttgcgcggtg gaggtgttcg
      301 ggctcctgga ggacgaggaa aattcacgaa ttgtgagagt aagagttata gccggaatag
      361 gccttgccaa gaaggatata ttgggagcta gtgatcctta cgtgagagtg acgttatatg
      421 acccaatgaa tggagttctt acaagtgtgc aaacaaaaac cattaaaaag agtttgaatc
      481 caaagtggaa tgaagaaata ttattcagag ttcatcctca gcagcaccgg cttctttttg
      541 aagtgtttga cgaaaaccga ttgacaagag atgatttcct aggtcaagtg gatgttccac
      601 tttatccatt accgacagaa aatccaagat tggagagacc atatacattt aaggattttg
      661 ttcttcatcc aagaagtcac aaatcaagag ttaaaggtta tctgagacta aaaatgactt
      721 atttacctaa aaccagtggc tcagaagatg ataatgcaga acaggctgag gattg
//
