LOCUS       sequence-298971-        5912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bait vector ERECTA C6_pECIA2 should be used with prey vector ERECTA 
            C6_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: ERECTA C6_pECIA2
FEATURES             Location/Qualifiers
     source          1..5912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             2165..2188
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2894..2935
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2945..2962
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2985..3002)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3035..3169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3059..3078)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3113..3132
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3313..3329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3313..3329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3326..3348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3337..3353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3361..3367,3368..3385,3386..3391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3406..3427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3544..3561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3715..4303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3795..3814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4474..5265,5266..5334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5097..5116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5335..5439)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5507..5525
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5563..5585)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5685..5704
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(5898..5912,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg tcagaggagg gagcaacgtt
      541 gctggagatt aagaagtcat tcaaagatgt gaacaatgtt ctttatgact ggacaacttc
      601 accttcttcg gattattgtg tctggagagg tgtgtcttgt gaaaatgtca ccttcaatgt
      661 tgttgctctt aatttgtcag atttgaatct tgatggagaa atctcacctg ctattggaga
      721 tctcaagagt ctcttgtcaa ttgatctgcg aggtaatcgc ttgtctggac aaatccctga
      781 tgagattggt gactgttctt ctttgcaaaa cttagactta tccttcaatg aattaagtgg
      841 tgacataccg ttttcgattt cgaagttgaa gcaacttgag cagctgattc tgaagaataa
      901 ccaattgata ggaccgatcc cttcaacact ttcacagatt ccaaacctga aaattctgga
      961 cttggcacag aataaactca gtggtgagat accaagactt atttactgga atgaagttct
     1021 tcagtatctt gggttgcgag gaaacaactt agtcggtaac atttctccag atttgtgtca
     1081 actgactggt ctttggtatt ttgacgtaag aaacaacagt ttgactggta gtatacctga
     1141 gacgatagga aattgcactg ccttccaggt tttggacttg tcctacaatc agctaactgg
     1201 tgagatccct tttgacatcg gcttcctgca agttgcaaca ttatcattgc aaggcaatca
     1261 actctctggg aagattccat cagtgattgg tctcatgcaa gcccttgcag tcttagatct
     1321 aagtggcaac ttgttgagtg gatctattcc tccgattctc ggaaatctta ctttcaccga
     1381 gaaattgtat ttgcacagta acaagctgac tggttcaatt ccacctgagc ttggaaacat
     1441 gtcaaaactc cattacctgg aactcaatga taatcatctc acgggtcata taccaccaga
     1501 gcttgggaag cttactgact tgtttgatct gaatgtggcc aacaatgatc tggaaggacc
     1561 tatacctgat catctgagct cttgcacaaa tctaaacagc ttaaatgttc atgggaacaa
     1621 gtttagtggc actatacccc gagcatttca aaagctagaa agtatgactt accttaatct
     1681 gtccagcaac aatatcaaag gtccaatccc ggttgagcta tctcgtatcg gtaacttaga
     1741 tacattggat ctttccaaca acaagataaa tggaatcatt ccttcttccc ttggtgattt
     1801 ggagcatctt ctcaagatga acttgagtag aaatcatata actggtgtag ttccaggcga
     1861 ctttggaaat ctaagaagca tcatggaaat agatctttca aataatgata tctctggccc
     1921 aattccagaa gagcttaacc aattacagaa cataattttg ctgagactgg aaaataataa
     1981 cctgactggt aatgttggtt cattagccaa ctgtctcagt ctcactgtat tgaatgtatc
     2041 tcataacaac ctcgtaggtg atatccctaa gaacaataac ttctcaagat tttcaccaga
     2101 cagcttcatt ggcaatcctg gtctttgcgg tagttggcta aactcaccgt gtcatgattc
     2161 tcgtctggag gtgctgttcc agggaccagg gagcgacaaa actcacacat gcccaccgtg
     2221 cccagcacct gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga
     2281 caccctcatg atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga
     2341 agaccctgag gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac
     2401 aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct
     2461 gcaccaggac tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc
     2521 agcccccatc gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta
     2581 caccctgccc ccatcccggg aggagatgac caagaaccag gtcagcctga cctgcctggt
     2641 caaaggcttc tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa
     2701 caactacaag accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa
     2761 gctcaccgtg gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca
     2821 tgagggtctg cacaaccact acacgcagaa gagcctctcc ctgtctccgg gtaaatctag
     2881 agggcccttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     2941 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
     3001 taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa gatacattga
     3061 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3121 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3181 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     3241 aaacctctac aaatgtggta tggctgatta tgatcagtcg acctgcaggc atgcaagctt
     3301 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3361 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     3421 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3481 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3541 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3601 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3661 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3721 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3781 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3841 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3901 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3961 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4021 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4081 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4141 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4201 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4261 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4321 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4381 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4441 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4501 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4561 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4621 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4681 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4741 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4801 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4861 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4921 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4981 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5041 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5101 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5161 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5221 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5281 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5341 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5401 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5461 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     5521 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     5581 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     5641 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     5701 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     5761 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     5821 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     5881 ggtaacgcca gggttttccc agtcacgacg tt
//
