LOCUS       sequence-298973-        3627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1310.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1310
FEATURES             Location/Qualifiers
     source          1..3627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        238..256
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     238..255
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      962..971
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      966..975
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1005..1024)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1006..1024)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1031..1048)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1031..1047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1040..1062)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1188..1643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1275..1294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1485..1506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1721..1825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1826..1894,1895..2686)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2044..2063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2857..3445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3346..3365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3599..3616
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctatt
      241 taggtgacac tatagaatac tcaagctatg catccaacgc gttgggagct ctcccatatg
      301 gtcgacctgc aggcggccgc gaattcacta gtgatttgtt cctccacaac accaaagatg
      361 atttatgacc ttttcagaag gcacaaaacc atctcttttg ggggttgcat cactcagatc
      421 ttttttatcc atgcagtagg tggcaccgag atggtgctgc tcatagccat ggcgtttgac
      481 cgatatgttg ccatatgtaa gcctctgcac tacctgacca tcatgagccc acaaaagtgt
      541 attttgattt tggttgcttc ttggattatt ggcttcatcc actcagtgac tcagctgagt
      601 tttgtggtag acttgccctt ctgtggccct aatgaactag acagcttttt ctgtgacctt
      661 cctaggttta ttaaacttgc ttgtgtggac acatacacac tggggtttat ggttactgct
      721 aacagtgggt tcatttctgt ggcctccttt ttaattctga tcatctctta catatttatt
      781 ttggtgactg ttcaaaagaa atctttgggt agtttaggta aggccctctc cactttgtca
      841 gctcatgtca ttgtggtagt tttgttcttt ggacccttaa tcttcttcta tacatggcca
      901 tttccaacat cacatctgga taaatttctt gccatctttg atgcagaatc gaattcccgc
      961 ggccgccatg gcggccggga gcatgcgacg tcgggcccaa ttcgccctat agtgagtcgt
     1021 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1081 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1141 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
     1201 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1261 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1321 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1381 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1501 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1561 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1621 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     1681 atttcacacc gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1741 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1801 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1861 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1921 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1981 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2041 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2101 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2161 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2221 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2281 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2341 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2401 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2461 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2521 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2581 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2641 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2701 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2761 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2821 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2881 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2941 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3001 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3061 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3121 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3181 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3241 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3301 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3361 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3421 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3481 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3541 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3601 cgagtcagtg agcgaggaag cggaaga
//
