LOCUS       sequence-29899-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  TITLE     Howley Lab Plasmids
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHAGE-P CMVt N-HA hNedd4 (C867A) DN
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     39..63
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             163..189
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    205..229
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
       61 atcgcctgga gacgccatcc acgctgnttt tgacctccat agaagacacc gggaccgatc
      121 cagcctccgg actctagcgt ttaaacgcta gccaccatgg cgtacccata cgatgttcca
      181 gattacgctg ctcgaggtta tcaaacaagt ttgtacaaaa aagcaggctc cgcggccgcc
      241 cccttcacca tggcaacttg cgcggtggag gtgttcgggc tcctggagga cgaggaaaat
      301 tcacgaattg tgagagtaag agttatagcc ggaataggcc ttgccaagaa ggatatattg
      361 ggagctagtg atccttacgt gagagtgacg ttatatgacc caatgaatgg agttcttaca
      421 agtgtgcaaa caaaaaccat taaaaagagt ttgaatccaa agtggaatga agaaatatta
      481 ttcagagttc atcctcagca gcaccggctt ctttttgaag tgtttgacga aaaccgattg
      541 acaagagatg atttcctagg tcaagtggat gttccacttt atccattacc gacagaaaat
      601 ccaagattgg agagaccata tacatttaag gattttgttc ttcatccaag aagtcacaaa
      661 tcaagagtta aaggttatct gagactaaaa atgacttatt tacctaaaac cagtggctca
      721 gagatgatat gcagaacagg ctgaggatta gagcggtggt gt
//
